Abstract
Changes in epigenetic states can allow individuals to cope with environmental changes. If such changes are heritable, this may lead to epigenetic adaptation. Thus, it is likely that in sessile organisms such as plants, part of the spatial epigenetic variation found across individuals will reflect the environmental heterogeneity within populations. The departure of the spatial epigenetic structure from the baseline genetic variation can help in understanding the value of epigenetic regulation in species with different breadth of optimal environmental requirements. Here, we hypothesise that in plants with narrow environmental requirements, epigenetic variability should be less structured in space given the lower variability in suitable environmental conditions. We performed a multispecies study that considered seven pairs of congeneric plant species, each encompassing a narrow endemic with habitat specialisation and a widespread species. In three populations per species we used AFLP and methylation-sensitive AFLP markers to characterise the spatial genetic and epigenetic structures. Narrow endemics showed a significantly lower epigenetic than genetic differentiation between populations. Within populations, epigenetic variation was less spatially structured than genetic variation, mainly in narrow endemics. In these species, structural equation models revealed that such pattern was associated to a lack of correlation between epigenetic and genetic information. Altogether, these results show a greater decoupling of the spatial epigenetic variation from the baseline spatial genetic pattern in endemic species. These findings highlight the value of studying genetic and epigenetic spatial variation to better understand habitat specialisation in plants.
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All data are available in Figshare. https://doi.org/10.6084/m9.figshare.24898461.
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Acknowledgements
We are very grateful to Pilar Bazaga and Esmeralda López-Perea for laboratory assistance. Noelia Zara for her help in sampling Erodium individuals. We thank the Consejería de Medio Ambiente, Junta de Andalucía, for authorising this research. This work was supported by the Consejería de Innovación Ciencia y Empresa, Junta de Andalucía [P18-FR-4413] and the Ministerio de Ciencia e Innovación, Spanish Government [PID2019-104365GB-I00/AEI/10.13039/501100011033 and SUMHAL-LIFEWATCH-2019-09-CSIC-4, through European Regional Development Funds POPE 2014-2020].
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CA, CMH and MM designed the methodology and collected the data; CA, CMH, MM and JV elaborated the hypotheses; JV analysed the data and led the writing of the manuscript. All authors contributed to the revision of the manuscript and gave their final approval for publication.
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Valverde, J., Medrano, M., Herrera, C.M. et al. Comparative epigenetic and genetic spatial structure in Mediterranean mountain plants: a multispecies study. Heredity 132, 106–116 (2024). https://doi.org/10.1038/s41437-024-00668-3
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DOI: https://doi.org/10.1038/s41437-024-00668-3