Article
- The EMBO Journal (2005) 24, 2436 - 2446
- doi:10.1038/sj.emboj.7600705
Published online: 2 June 2005
Subject Category:
Etd1p is a novel protein that links the SIN cascade with cytokinesis
Rafael R Daga1,2,a, Aurelia Lahoz1, Manuel J Muñoz1, Sergio Moreno2 and Juan Jimenez1
- Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide, Carretera de Utrera Km1, Sevilla, Spain
- Centro de Investigación del Cáncer, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
Correspondence to:
Juan Jimenez, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide, Carretera de Utrera Km1, 41013 Sevilla, Spain. Tel./Fax: +34 954 349 376; E-mail: jjimmar@upo.es
aPresent address: Department of Microbiology, Columbia University, New York, NY 10032, USA
Received 20 January 2005; Accepted 10 May 2005
Abstract
In animal cells, cytokinesis occurs by constriction of an actomyosin ring. In fission yeast cells, ring constriction is triggered by the septum initiation network (SIN), an SPB-associated GTPase-regulated kinase cascade that coordinates exit from mitosis with cytokinesis. We have identified a novel protein, Etd1p, required to trigger actomyosin ring constriction in fission yeasts. This protein is localised at the cell tips during interphase. In mitosis, it relocates to the medial cortex region and, coincident with cytokinesis, it assembles into the actomyosin ring by association to Cdc15p. Relocation of Etd1p from the plasma membrane to the medial ring is triggered by SIN signalling and, reciprocally, relocation of the Sid2p–Mob1p kinase complex from the SPB to the division site, a late step in the execution of the SIN, requires Etd1p. These results suggest that Etd1p coordinates the mitotic activation of SIN with the initiation of actomyosin ring constriction. Etd1p peaks during cytokinesis and is degraded by the ubiquitin-dependent 26S-proteasome pathway at the end of septation, providing a mechanism to couple inactivation of SIN to completion of cytokinesis.
Keywords:
- actomyosin ring,
- cell division,
- cytokinesis,
- Etd1p,
- SIN
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