Article
- The EMBO Journal (2006) 25, 811 - 821
- doi:10.1038/sj.emboj.7600966
Published online: 26 January 2006
Subject Categories:
Transcription regulation from a TATA and INR-less promoter: spatial segregation of promoter function
Anuja A George, Manish Sharma, Badri N Singh, Naresh C Sahoo and Kanury VS Rao
- Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
Correspondence to:
Kanury VS Rao, Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India. Tel.: +91 11 2617 6680; Fax: +91 11 267 5114; E-mail: kanury.rao@gmail.com
Received 18 October 2005; Accepted 23 December 2005
Abstract
The mode of regulation of class II genes that lack the known core promoter elements is presently unclear. Here, we studied one such example, the murine CD80 gene. An unusual mechanism was revealed wherein the pre-initiation complex (PIC) first assembled on an upstream, NF-
B enhancer element. Notably, this assembly occurred independent of contributions from the core promoter domain, and resulted in a PIC that was competent for transcription initiation. Positioning was subsequently achieved by exploiting the intrinsic architecture of the promoter, by virtue of which the tethered PIC was spatially juxtaposed with the transcription initiation site. Bridging interactions then ensued, through protein–protein contacts, which then enabled the elongation phase of CD80 transcription.
Keywords:
- activation,
- CD80,
- transcription
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