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EMBO reports 6, 12, 1188–1193 (2005)
doi:10.1038/sj.embor.7400571
Published online: 11 November 2005
RNA polyadenylation in Archaea: not observed in Haloferax while the exosome polynucleotidylates RNA in Sulfolobus
Victoria Portnoy1, Elena Evguenieva-Hackenberg2, Franziska Klein2, Pamela Walter2, Esben Lorentzen3, Gabriele Klug2 & Gadi Schuster1
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1 Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
2 Institut fur Mikrobiologie und Molekularbiologie, Justus-Liebig-Universitat Giessen, Heinrich-Buff-Ring 26, 35392 Giessen, Germany
3 EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
To whom correspondence should be addressed
Gadi Schuster Tel: +972 4 829 3171; Fax: +972 4 829 5587; E-mail: gadis@tx.technion.ac.il
Received 17 June 2005; Accepted 26 September 2005; Published online 11 November 2005.
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Abstract
The addition of poly(A) tails to RNA is a phenomenon common to all organisms examined so far. No homologues of the known polyadenylating enzymes are found in Archaea and little is known concerning the mechanisms of messenger RNA degradation in these organisms. Hyperthermophiles of the genus Sulfolobus contain a protein complex with high similarity to the exosome, which is known to degrade RNA in eukaryotes. Halophilic Archaea, however, do not encode homologues of these eukaryotic exosome components. In this work, we analysed RNA polyadenylation and degradation in the archaea Sulfolobus solfataricus and Haloferax volcanii. No RNA polyadenylation was detected in the halophilic archaeon H. volcanii. However, RNA polynucleotidylation occurred in hyperthermophiles of the genus Sulfolobus and was mediated by the archaea exosome complex. Together, our results identify the first organism without RNA polyadenylation and show a polyadenylation activity of the archaea exosome.
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