TABLE 1
FROM:
Membrane identity and GTPase cascades regulated by toggle and cut-out switches
Perla Del Conte-Zerial, Lutz Brusch, Jochen C Rink, Claudio Collinet, Yannis Kalaidzidis, Marino Zerial & Andreas Deutsch
doi:10.1038/msb.2008.45
BACK TO ARTICLETable 1: Experimental tests (numbers, see text for details), models and model predictions
| Experimental observations | References | Predictions of model 1 (toggle switch) | Predictions of model 2 (cut-out switch) |
|---|---|---|---|
By comparing the model predictions with four independent experimental results, model 2, the cut-out switch, is corroborated by the present data. Figure 4C and D shows the numerical simulations for models 1 and 2, respectively. Figure 3 shows the statistical analysis of the time courses of 23 endosomes that undergo conversion: [Rab5](time) is increasing towards the conversion event. | |||
| (1) Inhibition of GTP hydrolysis by Rab5 yields persistence of Rab5 and induction of Rab7, hence higher colocalization | Rink et al (2005) | Lower colocalization | Higher colocalization |
| (2) Inhibition of GDP/GTP exchange on Rab5 yields less degradation | Papini et al (1997) and Vitelli et al (1997) | More degradation | Less degradation |
| (3) Density/radius diagram of Rab5 on endosomes shows increasing Rab5 density | Rink et al (2005) | Decrease | Increase |
| (4) [Rab5](time) is increasing towards the conversion event | This work, Figure 3 | Decrease | Increase |
