Nature 324, 34 - 38 (06 November 1986); doi:10.1038/324034a0

Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene

Kirk R. Thomas & Mario R. Capecchi

Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA

Injection of homologous DNA sequences into nuclei of cultured mammalian cells induces mutations in the cognate chromosomal gene. It appears that these mutations result from incorrect repair of a heteroduplex formed between the introduced and the chromosomal sequence. This phenomenon is termed 'heteroduplex induced mutagenesis' The high frequency of these events suggests that this method may prove useful for introducing mutations into specific mammalian genes.

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