Q2 finance fall-off - p850

Stacy Lawrence

doi:10.1038/nbt0808-850

Full Text - Q2 finance fall-off | PDF (291 KB) - Q2 finance fall-off

Do biomaterials really mean business? - pp851 - 853

Emily Waltz

doi:10.1038/nbt0808-851

Receiving only a fraction of the investment set aside for biofuels, biomaterials will likely have to piggyback on technological advances in energy production to compete with petroleum-based products in the marketplace. Emily Waltz reports.

Abstract - | Full Text - Do biomaterials really mean business? | PDF (153 KB) - Do biomaterials really mean business?

The economics of licensing contracts - pp855 - 857

Richard Mason, Nicos Savva & Stefan Scholtes

doi:10.1038/bioe.2008.7

PDF (120 KB) - The economics of licensing contracts

GMO testing—trade, labeling or safety first? - pp858 - 859

Arne Holst-Jensen

doi:10.1038/nbt0808-858

Full Text - GMO testing—trade, labeling or safety first? | PDF (191 KB) - GMO testing—trade, labeling or safety first?

First evaluation of the European hESCreg - pp859 - 860

Joeri Borstlap, Glyn Stacey, Andreas Kurtz, Anja Elstner, Alexander Damaschun, Begoña Arán & Anna Veiga

doi:10.1038/nbt0808-859

Full Text - First evaluation of the European hESCreg | PDF (197 KB) - First evaluation of the European hESCreg

Guidelines for reporting the use of mass spectrometry in proteomics - pp860 - 861

Chris F Taylor, Pierre-Alain Binz, Ruedi Aebersold, Michel Affolter, Robert Barkovich, Eric W Deutsch, David M Horn, Andreas Hühmer, Martin Kussmann, Kathryn Lilley, Marcus Macht, Matthias Mann, Dieter Müller, Thomas A Neubert, Janice Nickson, Scott D Patterson, Roberto Raso, Kathryn Resing, Sean L Seymour, Akira Tsugita, Ioannis Xenarios, Rong Zeng & Randall K Julian, Jr

doi:10.1038/nbt0808-860

Full Text - Guidelines for reporting the use of mass spectrometry in proteomics | PDF (154 KB) - Guidelines for reporting the use of mass spectrometry in proteomics | Supplementary information

Guidelines for reporting the use of mass spectrometry informatics in proteomics - p862

Pierre-Alain Binz, Robert Barkovich, Ronald C Beavis, David Creasy, David M Horn, Randall K Julian, Jr, Sean L Seymour, Chris F Taylor & Yves Vandenbrouck

doi:10.1038/nbt0808-862

Full Text - Guidelines for reporting the use of mass spectrometry informatics in proteomics | PDF (77 KB) - Guidelines for reporting the use of mass spectrometry informatics in proteomics | Supplementary information

Guidelines for reporting the use of gel electrophoresis in proteomics - pp863 - 864

Frank Gibson, Leigh Anderson, Gyorgy Babnigg, Mark Baker, Matthias Berth, Pierre-Alain Binz, Andy Borthwick, Phil Cash, Billy W Day, David B Friedman, Donita Garland, Howard B Gutstein, Christine Hoogland, Neil A Jones, Alamgir Khan, Joachim Klose, Angus I Lamond, Peter F Lemkin, Kathryn S Lilley, Jonathan Minden, Nicholas J Morris, Norman W Paton, Michael R Pisano, John E Prime, Thierry Rabilloud, David A Stead, Chris F Taylor, Hans Voshol, Anil Wipat & Andrew R Jones

doi:10.1038/nbt0808-863

Full Text - Guidelines for reporting the use of gel electrophoresis in proteomics | PDF (157 KB) - Guidelines for reporting the use of gel electrophoresis in proteomics | Supplementary information

The PSI-MOD community standard for representation of protein modification data - pp864 - 866

Luisa Montecchi-Palazzi, Ron Beavis, Pierre-Alain Binz, Robert J Chalkley, John Cottrell, David Creasy, Jim Shofstahl, Sean L Seymour & John S Garavelli

doi:10.1038/nbt0808-864

Full Text - The PSI-MOD community standard for representation of protein modification data | PDF (101 KB) - The PSI-MOD community standard for representation of protein modification data

The London Agreement and the future of parallel imports - pp877 - 879

Justine Muir

doi:10.1038/nbt0808-877

Are European patents really getting cheaper, and if so, what effect will this have on parallel or so-called gray imports?

Abstract - | Full Text - The London Agreement and the future of parallel imports | PDF (250 KB) - The London Agreement and the future of parallel imports

Recent patent applications in DNA synthesis - p880

doi:10.1038/nbt0808-880

Full Text - Recent patent applications in DNA synthesis | PDF (59 KB) - Recent patent applications in DNA synthesis

Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project - pp889 - 896

Chris F Taylor, Dawn Field, Susanna-Assunta Sansone, Jan Aerts, Rolf Apweiler, Michael Ashburner, Catherine A Ball, Pierre-Alain Binz, Molly Bogue, Tim Booth, Alvis Brazma, Ryan R Brinkman, Adam Michael Clark, Eric W Deutsch, Oliver Fiehn, Jennifer Fostel, Peter Ghazal, Frank Gibson, Tanya Gray, Graeme Grimes, John M Hancock, Nigel W Hardy, Henning Hermjakob, Randall K Julian, Jr, Matthew Kane, Carsten Kettner, Christopher Kinsinger, Eugene Kolker, Martin Kuiper, Nicolas Le Novère, Jim Leebens-Mack, Suzanna E Lewis, Phillip Lord, Ann-Marie Mallon, Nishanth Marthandan, Hiroshi Masuya, Ruth McNally, Alexander Mehrle, Norman Morrison, Sandra Orchard, John Quackenbush, James M Reecy, Donald G Robertson, Philippe Rocca-Serra, Henry Rodriguez, Heiko Rosenfelder, Javier Santoyo-Lopez, Richard H Scheuermann, Daniel Schober, Barry Smith, Jason Snape, Christian J Stoeckert, Jr, Keith Tipton, Peter Sterk, Andreas Untergasser, Jo Vandesompele & Stefan Wiemann

doi:10.1038/nbt.1411

The Minimum Information for Biological and Biomedical Investigations (MIBBI) project aims to foster the coordinated development of minimum-information checklists and provide a resource for those exploring the range of extant checklists.

Full Text - Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project | PDF (233 KB) - Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project | Supplementary information

What is the expectation maximization algorithm? - pp897 - 899

Chuong B Do & Serafim Batzoglou

doi:10.1038/nbt1406

The expectation maximization algorithm arises in many computational biology applications that involve probabilistic models. What is it good for, and how does it work?

Abstract - | Full Text - What is the expectation maximization algorithm? | PDF (856 KB) - What is the expectation maximization algorithm? | Supplementary information

Neutralizing antibodies to therapeutic enzymes: considerations for testing, prevention and treatment - pp901 - 908

Jinhai Wang, Jay Lozier, Gibbes Johnson, Susan Kirshner, Daniela Verthelyi, Anne Pariser, Elizabeth Shores & Amy Rosenberg

doi:10.1038/nbt.1484

Abstract - | Full Text - Neutralizing antibodies to therapeutic enzymes: considerations for testing, prevention and treatment | PDF (1,124 KB) - Neutralizing antibodies to therapeutic enzymes: considerations for testing, prevention and treatment

Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita - pp909 - 915

Pierre Abad, Jérôme Gouzy, Jean-Marc Aury, Philippe Castagnone-Sereno, Etienne G J Danchin, Emeline Deleury, Laetitia Perfus-Barbeoch, Véronique Anthouard, François Artiguenave, Vivian C Blok, Marie-Cécile Caillaud, Pedro M Coutinho, Corinne Dasilva, Francesca De Luca, Florence Deau, Magali Esquibet, Timothé Flutre, Jared V Goldstone, Noureddine Hamamouch, Tarek Hewezi, Olivier Jaillon, Claire Jubin, Paola Leonetti, Marc Magliano, Tom R Maier, Gabriel V Markov, Paul McVeigh, Graziano Pesole, Julie Poulain, Marc Robinson-Rechavi, Erika Sallet, Béatrice Ségurens, Delphine Steinbach, Tom Tytgat, Edgardo Ugarte, Cyril van Ghelder, Pasqua Veronico, Thomas J Baum, Mark Blaxter, Teresa Bleve-Zacheo, Eric L Davis, Jonathan J Ewbank, Bruno Favery, Eric Grenier, Bernard Henrissat, John T Jones, Vincent Laudet, Aaron G Maule, Hadi Quesneville, Marie-Noëlle Rosso, Thomas Schiex, Geert Smant, Jean Weissenbach & Patrick Wincker

doi:10.1038/nbt.1482

Biological control of the root-knot nematode Meloidogyne incognita, one of the world's most destructive crop pathogens, presents a major opportunity for safely improving global agricultural yields. Its 86-Mb genome—the first to be sequenced for a strictly parthenogenetic species—provides a blueprint to design new strategies for plant protection.

Abstract - | Full Text - Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita | PDF (580 KB) - Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita | Supplementary information

See also: News and Views by McCarter

A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types - pp916 - 924

Marius Wernig, Christopher J Lengner, Jacob Hanna, Michael A Lodato, Eveline Steine, Ruth Foreman, Judith Staerk, Styliani Markoulaki & Rudolf Jaenisch

doi:10.1038/nbt1483

Generating induced pluripotent stem (iPS) cells is still an inefficient process, in part because the delivery of reprogramming factors by retroviral vectors yields cell populations that are genetically heterogeneous. Wernig et al. increase efficiency by producing iPS-cell chimeric mice from which they isolate cells bearing identical proviral insertions that support drug-inducible reprogramming.

Abstract - | Full Text - A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types | PDF (1,515 KB) - A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types | Supplementary information

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index - pp925 - 932

Jagath R Junutula, Helga Raab, Suzanna Clark, Sunil Bhakta, Douglas D Leipold, Sylvia Weir, Yvonne Chen, Michelle Simpson, Siao Ping Tsai, Mark S Dennis, Yanmei Lu, Y Gloria Meng, Carl Ng, Jihong Yang, Chien C Lee, Eileen Duenas, Jeffrey Gorrell, Viswanatham Katta, Amy Kim, Kevin McDorman, Kelly Flagella, Rayna Venook, Sarajane Ross, Susan D Spencer, Wai Lee Wong, Henry B Lowman, Richard Vandlen, Mark X Sliwkowski, Richard H Scheller, Paul Polakis & William Mallet

doi:10.1038/nbt.1480

Systemic toxicity associated with heterogeneity in the stoichiometries and sites of drug attachment is a major hurdle to developing antibody-drug conjugates (ADCs) for cancer therapy. Junutula et al. engineer cysteine residues in constant domains to produce near-homogenous ADCs that are better tolerated than conventional ADCs, without any loss of antitumor activity.

Abstract - | Full Text - Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index | PDF (357 KB) - Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index | Supplementary information

See also: News and Views by Damle

A small molecule enhances RNA interference and promotes microRNA processing - pp933 - 940

Ge Shan, Yujing Li, Junliang Zhang, Wendi Li, Keith E Szulwach, Ranhui Duan, Mohammad A Faghihi, Ahmad M Khalil, Lianghua Lu, Zain Paroo, Anthony W S Chan, Zhangjie Shi, Qinghua Liu, Claes Wahlestedt, Chuan He & Peng Jin

doi:10.1038/nbt.1481

Little is known about the regulation of RNA interference (RNAi). Shan et al. constructed a reporter system to monitor RNAi activity and identified a small molecule that increases RNAi by facilitating the interaction between small RNAs and a protein involved in small RNA loading and processing.

Abstract - | Full Text - A small molecule enhances RNA interference and promotes microRNA processing | PDF (345 KB) - A small molecule enhances RNA interference and promotes microRNA processing | Supplementary information

Global identification of microRNA–target RNA pairs by parallel analysis of RNA ends - pp941 - 946

Marcelo A German, Manoj Pillay, Dong-Hoon Jeong, Amit Hetawal, Shujun Luo, Prakash Janardhanan, Vimal Kannan, Linda A Rymarquis, Kan Nobuta, Rana German, Emanuele De Paoli, Cheng Lu, Gary Schroth, Blake C Meyers & Pamela J Green

doi:10.1038/nbt1417

The targets of a microRNA (miRNA) are usually identified by computational analysis of sequences complementary to the miRNA. Working with inflorescence tissue of Arabidopsis, German et al. devise an experimental approach in which the products of miRNA-mediated cleavage are sequenced and used to identify miRNA–target RNA pairs.

First Paragraph - | Full Text - Global identification of microRNA–target RNA pairs by parallel analysis of RNA ends | PDF (643 KB) - Global identification of microRNA–target RNA pairs by parallel analysis of RNA ends | Supplementary information

See also: News and Views by Henderson & Jacobsen

Westernizing Eastern-bloc science - pp949 - 950

Quirin Schiermeier

doi:10.1038/nbt0808-949

Scientists in newer member countries are learning how to use what the European Union offers.

Abstract - | Full Text - Westernizing Eastern-bloc science | PDF (182 KB) - Westernizing Eastern-bloc science

People - p952

doi:10.1038/nbt0808-952

Full Text - People | PDF (107 KB) - People