Supplementary information
From the following article
Adenosine A2A receptor mediates microglial process retraction
Anna G Orr, Adam L Orr, Xiao-Jiang Li, Robert E Gross & Stephen F Traynelis
Nature Neuroscience 12, 872 - 878 (2009) Published online: 14 June 2009
doi:10.1038/nn.2341
Supplementary Text and Figures
Supplementary Figures 1–6
Supplementary Video 1
ATP repels LPS-treated microglia. Time-lapse three-dimensional reconstruction of a cultured LPS-activated mouse microglia migrating away from an ATP-filled micropipette positioned just beyond right edge of screen (pipette not shown).
Supplementary Video 2
ATP induces process extension in untreated microglia. Time-lapse three-dimensional reconstruction of a mouse microglia exhibiting process extension toward the periphery of field during bath application of ATP.
Supplementary Video 3
ATP induces process retraction in LPS-treated microglia. Time-lapse three-dimensional reconstruction of an LPS-activated mouse microglia exhibiting process retraction during bath application of ATP.
Supplementary Video 4
ATP inhibits microglial process motion. Three-dimensional tracking (red traces) of cell processes of an LPS-activated mouse microglia. Bath application of ATP inhibited process motility.
Supplementary Video 5
ATP attracts untreated human microglia. Cultured human microglia migrated toward and engulfed an ATP-filled micropipette at right.
Supplementary Video 6
ATP repels LPS-treated human microglia. Cultured LPS-activated human microglia migrated away from an ATP-filled micropipette at right (pipette chases cell across field).
Supplementary Video 7
Acute injury attracts untreated microglia in vitro. Untreated GFP-positive mouse microglia extended their processes toward acutely injured astrocytes. Mechanical injury (right edge) was induced with a micropipette at approximately 9 min (n = 3).
Supplementary Video 8
Acute injury repels LPS-treated microglia in vitro. LPS-activated GFP-positive mouse microglia retracted its processes following astrocyte injury. Mechanical injury (right edge) was induced with a micropipette at approximately 8 min (n = 6).
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