Nature Immunology 7, 338 - 343 (2006)
Published online: 20 March 2006; | doi:10.1038/ni1323
Thymic microenvironments for T cell differentiation and selectionEna Ladi, Xinye Yin, Tatyana Chtanova
& Ellen A Robey Supplementary Video 1 (avi 26.6M)
Slow, meandering migration of cortical thymocytes expressing diverse TCRs. Real-time imaging of GFP+ thymocytes in the cortex of intact thymic lobes. Green represents the fluorescent signal from GFP thymocytes and colored lines represent the position of individual cells over time. Data set (x, y, z, time) has the dimensions 164 microns  164 microns 40 microns 25 minutes. Corresponds to Fig. 2a. A typical slow meandering thymocyte (black track near center), and a rare rapid directional thymocyte (black track near bottom), are highlighted. Supplementary Video 2 (avi 26M)
Rapid, directional migration of thymocytes expressing a positively selected TCR. Real-time imaging of thymocytes expressing GFP and a positively selected TCR (P14) in the cortex of intact thymic lobes. Green represents the fluorescent signal from GFP+ thymocytes and colored lines represent the position of individual cells over time. Data set (x, y, z, time) has the dimensions 164 microns 164 microns 40 microns 25 minutes. Corresponds to Fig. 2b. Note the large proportion of rapid, directional thymocytes.
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