Figure 3 - Foxo3a-A3 promotes differentiation of pre-B cells.

(a,b) Differentiation of pre-B cells expressing a constitutively active form of Foxo3a. SLP-65–deficient pre-B cells were transduced with a vector encoding a constitutively active form of Foxo3a (Foxo3a-A3–IRES–GFP) or the empty vector as a control and cultured either with (a) or without (b) IL-7 for 48 h. Differentiation of pre-B cells, indicated by cell size (left) and surface light chain expression (right) was measured by flow cytometry in GFP^- and the GFP⁺ population within each sample. The numbers indicate the percentages of cells in the respective regions. Data are representative of four independent experiments (a,b).