Supplementary information
From the following article
Boyd Butler, Diana H Kastendieck & John A Cooper
Nature Immunology 9, 887 - 897 (2008) Published online: 29 June 2008
doi:10.1038/ni.1630
Supplementary Text and Figures
Supplementary Figures 1–2 and Methods
Supplementary Movie 1
Primary NK cells expressing GFP-HS1 in contact with K562 target cells. Frame interval 1 min.
Supplementary Movie 2
Primary NK cells expressing GFP-HS1 in contact with K562 target cells. Frame interval 1 min.
Supplementary Movie 3
Adhesion of primary control NK cells on ICAM-1. Brightfield, frame interval 5 sec.
Supplementary Movie 4
Adhesion of primary control NK cells on Fn. Brightfield, frame interval 5 sec.
Supplementary Movie 5
GFP-Actin dynamics in control NKL cells on ICAM-1. TIRF frame interval 3 sec.
Supplementary Movie 6
GFP-HS1 dynamics in control NKL cells on ICAM-1. TIRF, frame interval 3 sec.
Supplementary Movie 7
Adhesion of HS1 knockdown primary NK cells on ICAM-1
Adhesion of primary NK cells on Fn. Brightfield, frame interval 5 sec.
Supplementary Movie 8
Adhesion of HS1 knockdown primary NK cells on Fn. Brightfield, frame interval 5 sec.
Supplementary Movie 9
GFP-Actin dynamics in HS1 knockdown NKL cells on ICAM-1. TIRF, frame interval 3 sec.
Supplementary Movie 10
Adhesion of HS1 knockdown primary NK cells expressing Y378F HS1 on ICAM-1. Brightfield, frame interval 5 sec.
Supplementary Movie 11
GFP-Actin dynamics in HS1 knockdown NKL cells expressing Y378F HS1 on ICAM-1. Brightfield, frame interval 5 sec.
Supplementary Movie 12
Adhesion of HS1 knockdown primary NK cells expressing Y397F HS1 on ICAM-1. Brightfield, frame interval 5 sec.
Supplementary Movie 13
GFP-Actin dynamics in HS1 knockdown NKL cells expressing Y397F HS1 on ICAM-1. TIRF, frame interval 3 sec.
Supplementary Movie 14
Chemotaxis of control primary NK cells on Fn. Brightfield, frame interval 1 min.
Supplementary Movie 15
Chemotaxis of HS1 knockdown primary NK cells on Fn. Brightfield, frame interval 1 min.
Supplementary Movie 16
Chemotaxis of HS1 knockdown primary NK cells expressing Y397F HS1on Fn. Brightfield, frame interval 1 min.
Supplementary Movie 17
Chemotaxis of HS1 knockdown primary NK cells expressing Y378F HS1 on Fn. Brightfield, frame interval 1 min.
Supplementary Movie 18
GFP-Actin dynamics during chemotaxis in control NKL cells on Fn. TIRF, frame interval 15 sec.
Supplementary Movie 19
GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells on Fn. TIRF, frame interval 15 sec.
Supplementary Movie 20
GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells expressing Y397F HS1 on Fn. TIRF, frame interval 15 sec.
Supplementary Movie 21
GFP-Actin dynamics during chemotaxis in HS1 knockdown NKL cells expressing Y378F HS1 on Fn. TIRF, frame interval 15 sec.
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