Nature Methods - CURRENT ISSUE : January 2008 - Vol 6 No 1
Latest highlights
ADVANCE ONLINE PUBLICATION
A sensor for ATP
Article by Berg et al.Yellen and colleagues present a ratiometric fluorescent sensor that reports the ATP:ADP concentration ratio in living cells. The sensor detected inhibition of cellular metabolism caused by transient removal of glucose from the cellular medium or by administration of a glycolytic inhibitor.
CURRENT ISSUE
Celebrate methods development with our Method of the Year 2008. Discover our winner, read about its development, understand its inner workings, and hear from experts about its impact on research. We have also included a selection of Methods to Watch for the years to come!
ADVANCE ONLINE PUBLICATION
Cell fusion on chip
Article by Skelley et al.A device that traps cells in micrometer-sized capture cups to efficiently achieve cell pairing and subsequent fusion is presented by Voldman and colleagues. The device is used to achieve reprogramming of mouse embryonic fibroblasts after fusion with embryonic stem cells.
ADVANCE ONLINE PUBLICATION
New transsynaptic viral tools
Brief Communication by Boldogkõi et al.Pseudorabies viruses encoding fluorescent proteins are powerful tools for mapping neuronal circuits. Roska and colleagues now present a series of pseudorabies virus strains encoding fluorescent sensors, for dissection of complex circuits with concurrent activity analysis while defining an analysis period during which the neurons are still healthy.
RESEARCH HIGHLIGHTS
Cell-specific mRNA profile
Targeted translational profilingFREE
To obtain the translational profile of particular cell types in the brain, researchers GFP-tagged a ribosomal protein under the control of a cell type–specific promoter. This allowed affinity purification of ribosomes with attached mRNAs from the targeted cells, without cell dissection.
Application Notes
Transferring DNA for Southern blotting in 7 minutes with the iBlot® Dry Blotting System
Go to the Application Notes database
See also this month:
Application Notes from Thermo Fisher Scientific, New Brunswick Scientific, BD Biosciences, PerkinElmer, Caprotec bioanalytics and Chroma.
