Nature Methods - CURRENT ISSUE : November 2009 - Vol 6 No 11
- 4C-ing rearrangements
- Improved reprogramming of human cells
- Visual proteomics of Leptospira interrogans
Latest highlights
FOCUS
NGS data analysis
Online FocusFREE
To extract a maximum of information from next-generation sequencing data, users have to navigate a maze of different software options. A series of Reviews guides readers through the analysis steps for different applications and explains how different algorithms handle these steps.
ANNOUNCEMENT
Method of the Year 2009
Visit our site to vote!FREE
Do you have an opinion on the methodology that drives biological research? We are soliciting votes and nominations from our readers for the Method of the Year 2009. Visit the link above and let us know what you think.
ADVANCE ONLINE PUBLICATION
A reporter of synaptic activity
Article by Dreosti et al.Localization of the genetically encoded calcium indicator GCaMP2 to presynaptic terminals conveys linear responsiveness over a wider range of spike frequencies. Lagnado and colleagues use the sensor to measure synaptic activity caused by spiking as well as graded voltage signals in vivo in zebrafish.
ADVANCE ONLINE PUBLICATION
An improved calcium indicator
Article by Tian et al.An improved version of the GCaMP genetically encoded calcium indicator, GCaMP3, is presented by Looger and colleagues. GCaMP3 performs better than existing genetically encoded calcium indicators in several assays and organisms, including in vivo imaging of neuronal signaling in worms, flies and mice.
ADVANCE ONLINE PUBLICATION
Optical circuit mapping
Article by Guo et al.There is much interest in mapping neural circuits in the context of living organisms. Ramanathan and colleagues combine neuronal stimulation with Channelrhodopsin-2 and calcium fluorescence imaging to study neural connections in C. elegans.
ADVANCE ONLINE PUBLICATION
Automated imaging in fish
Article by Gehrig et al.A set of methods for automated fluorescence imaging is used by Mueller and colleagues for high-throughput examination of reporter expression patterns in zebrafish embryos. They apply the approach to mapping promoter-enhancer interactions in this organism.
NEW ON METHAGORA
Consumer genetics
MethagoraFREE
The availability of direct-to-consumer genetic tests is expanding and generates much interest within the genetics and policy communities as well as among the public. Visit our blog to read our editorial on the topic.
As always, we welcome your comments on this and other posts!
Application Notes
SENSOLUX® stand-alone version: noninvasive determination of pH and DO in shake flasks
Go to the Application Notes database
See also this month:
Application Notes from BioTek, Epicentre Biotechnologies, Thermo Scientific, febit GmbH, Invitrogen, and Glen Mills.
