Nature Methods - CURRENT ISSUE : July 2009 - Vol 6 No 7
- Fast protein refolding with pressure jumps
- Transition metal ion FRET
- Embryonic stem cell lines for mouse resources
Latest highlights
CURRENT ISSUE
Detecting multiple T-cell populations
Article by Hadrup et al. Brief Communication by Newell et al. News & Views by Kedzierska et al.Using combinations of fluorescently labeled peptide–major histocompatability (pMHC) tetramers, two groups—Davis and colleagues, and Schumacher and colleagues—show that multiple T-cell populations with different antigen specificities can be monitored in parallel from small samples of human blood.
CURRENT ISSUE
Genome capture with PCR products
Brief Communication by Herman et al.Seidman and colleagues use concatenated PCR products as subgenomic traps in this targeted genome capture technique. Subsequent high-throughput sequencing allows the detection of nucleotide and structural variations in the captured genomic regions.
CURRENT ISSUE
Digital profiling of microRNA
Correspondence by Linsen et al.Next-generation sequencing is increasingly used for quantitative gene expression profiling. In this Correspondence, Cuppen and colleagues confirm its power for comparing relative expression differences, but state that absolute quantitation of small RNAs may be difficult because of library preparation biases.
CURRENT ISSUE
Finding relevant genes in RNAi screens
Correspondence by Kaplow et al.In this Correspondence, Perrimon and colleagues introduce RNAiCut, an automated program that overlaps RNA interference results with protein-protein interaction data to identify thresholds above which genes are enriched for functions relevant to the screen.
RESEARCH HIGHLIGHTS
Infrared fluorescent proteins
Fluorescent proteins: into the infraredFREE
Researchers developed an infrared-emitting fluorescent protein from a bacterial phytochrome scaffold. This first member of a new class of imaging probes has advantages over visible-wavelength fluorescent proteins for in vivo imaging.
RESEARCH HIGHLIGHTS
Gene transfer through grafting
Grafting as a potent molecular toolFREE
Grafted transgenic plants exchange large pieces of their DNA from both nuclear and chloroplast genomes at the immediate graft site. This intercellular gene transfer will yield clues about evolution and facilitate notoriously difficult chloroplast transformation.
NEW ON METHAGORA
Summer reading
MethagoraFREE
Our July issue is now online! Visit our blog to link to our editorial on science in fiction, and also to see rankings and commentary on the most downloaded Nature Methods papers in the past months.
As always, we welcome your comments on this and other posts!
Application Notes
Automated selection and collection of pluripotent stem cell colonies using the CellCelector™
Go to the Application Notes database
See also this month:
Application Notes from Cell Biosciences, Carestream Molecular Imaging, Sequenom, BMG Labtech, TTP Labtech, and Gatlik.
