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| Editorial | Top |
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Microcosmos p239
doi:10.1038/nmeth0405-239
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(32K)
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| Correspondence | Top |
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Different delivery methods—different expression profiles p241
Yuriy Fedorov, Alan King, Emily Anderson, Jon Karpilow, Diane Ilsley, William Marshall
& Anastasia Khvorova
doi:10.1038/nmeth0405-241
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(98K)
| Supplementary Information |
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| Research Highlights | Top |
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To affinity and beyond p243
Michael Eisenstein
doi:10.1038/nmeth0405-243
Abstract | Full text | PDF
(88K)
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Test tubes go microscopic pp244 - 245
Daniel Evanko
doi:10.1038/nmeth0405-244a
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(436K)
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The mighty micro pp244 - 245
Nicole Rusk
doi:10.1038/nmeth0405-244b
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(436K)
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News in Brief p245
doi:10.1038/nmeth0405-245
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(31K)
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Infiltrating the network p246
Michael Eisenstein
doi:10.1038/nmeth0405-246
Abstract | Full text | PDF
(27K)
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A need for speed p248
Allison Doerr
doi:10.1038/nmeth0405-248a
Abstract | Full text | PDF
(28K)
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Getting quantity and quality p248
Michael Eisenstein
doi:10.1038/nmeth0405-248b
Abstract | Full text | PDF
(28K)
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Bubbles without toil or trouble p249
Michael Eisenstein
doi:10.1038/nmeth0405-249
Abstract | Full text | PDF
(26K)
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| News and Views | Top |
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Measuring kinase activity: finding needles in a haystack pp251 - 252
Benjamin E Turk
doi:10.1038/nmeth0405-251
Abstract | Full text | PDF
(69K)
See also: Article by Shults et al. |
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Herpesviruses throw a curve ball: new insights into microRNA biogenesis and evolution pp252 - 254
Joel R Neilson
& Phillip A Sharp
doi:10.1038/nmeth0405-252
Abstract | Full text | PDF
(73K)
See also: Article by Pfeffer et al. |
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| Brief Communications | Top |
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In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag pp255 - 257
Lawrence W Miller, Yunfei Cai, Michael P Sheetz
& Virginia W Cornish
Published online: 23 March 2005 | doi:10.1038/nmeth749
Abstract | Full text | PDF
(265K)
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The hESC line Envy expresses high levels of GFP in all differentiated progeny pp259 - 260
Magdaline Costa, Mirella Dottori, Elizabeth Ng, Susan M Hawes, Koula Sourris, Pegah Jamshidi, Martin F Pera, Andrew G Elefanty
& Edouard G Stanley
Published online: 23 March 2005 | doi:10.1038/nmeth748
Abstract | Full text | PDF
(391K)
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| Articles | Top |
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Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells pp261 - 268
Monika Suchanek, Anna Radzikowska
& Christoph Thiele
Published online: 23 March 2005 | doi:10.1038/nmeth752
Abstract | Full text | PDF
(247K)
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Identification of microRNAs of the herpesvirus family pp269 - 276
Sébastien Pfeffer, Alain Sewer, Mariana Lagos-Quintana, Robert Sheridan, Chris Sander, Friedrich A Grässer, Linda F van Dyk, C Kiong Ho, Stewart Shuman, Minchen Chien, James J Russo, Jingyue Ju, Glenn Randall, Brett D Lindenbach, Charles M Rice, Viviana Simon, David D Ho, Mihaela Zavolan
& Thomas Tuschl
Published online: 16 February 2005 | doi:10.1038/nmeth746
Abstract | Full text | PDF
(376K)
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See also: News and Views by Neilson & Sharp |
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A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates pp277 - 284
Melissa D Shults, Kevin A Janes, Douglas A Lauffenburger
& Barbara Imperiali
Published online: 23 March 2005 | doi:10.1038/nmeth747
Abstract | Full text | PDF
(382K)
| Supplementary Information
See also: News and Views by Turk |
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Quantification of random genomic mutations pp285 - 290
Jason H Bielas
& Lawrence A Loeb
Published online: 23 March 2005 | doi:10.1038/nmeth751
Abstract | Full text | PDF
(296K)
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In vivo protein biotinylation for identification of organ-specific antigens accessible from the vasculature pp291 - 298
Jascha-N Rybak, Anna Ettorre, Brigitte Kaissling, Raffaella Giavazzi, Dario Neri
& Giuliano Elia
Published online: 23 March 2005 | doi:10.1038/nmeth745
Abstract | Full text | PDF
(654K)
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| Protocol | Top |
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Conjugation of fluorophores to tubulin pp299 - 303
John Peloquin, Yulia Komarova
& Gary Borisy
doi:10.1038/nmeth0405-299
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(120K)
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| Technology Feature | Top |
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Prime time for real-time PCR pp305 - 312
Laura Bonetta
doi:10.1038/nmeth0405-305
Abstract | Full text | PDF
(224K)
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| Application Notes | Top |
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ProbeLibrary: A new method for faster design and execution of quantitative real-time PCR pp313 - 316
Peter Mouritzen, Mikkel Noerholm, Peter S Nielsen, Nana Jacobsen, Christian Lomholt, Henrik M Pfundheller
& Niels Tolstrup
doi:10.1038/nmeth0405-313
Abstract | Full text | PDF
(312K)
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| Classic Protocols | Top |
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A look back: fast, cheap and under control p318
Michael Eisenstein
doi:10.1038/nmeth0405-318
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(24K)
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Calcium phosphate−mediated transfection of eukaryotic cells pp319 - 320
doi:10.1038/nmeth0405-319
Abstract | Full text | PDF
(30K)
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