Nature Methods
- 3, 715 - 719 (2006)
Published online: 23 August 2006; | doi:10.1038/nmeth924
Genome-scale loss-of-function screening with a lentiviral RNAi libraryDavid E Root1, Nir Hacohen1, 2, 3, William C Hahn1, 3, 4, Eric S Lander1, 3, 5, 6 & David M Sabatini1, 5, 61
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. 2
Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114, USA. 3
Harvard Medical School, Boston, Massachusetts 02115, USA. 4
Department of Medical Oncology, Center for Cancer Systems Biology, Center for Cancer Genome Discovery, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. 5
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. 6
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Correspondence should be addressed to David E Root droot@broad.mit.edu The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.
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