Nature Methods
- 4, 822 - 827 (2007)
Published online: 27 September 2007; | doi:10.1038/nmeth1092
Activity-based protein profiling for the functional annotation of enzymesKatherine T Barglow & Benjamin F Cravatt
The authors are at The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA. cravatt@scripps.edu
Activity-based protein profiling (ABPP), the use of active site-directed chemical probes to monitor enzyme function in complex biological systems, is emerging as a powerful post-genomic technology. ABPP probes have been developed for several enzyme classes and have been used to inventory enzyme activities en masse for a range of (patho) physiological processes. By presenting specific examples, we show here that ABPP provides researchers with a distinctive set of chemical tools to embark on the assignment of functions to many of the uncharacterized enzymes that populate eukaryotic and prokaryotic proteomes.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
