Brief Communication abstract
Nature Methods 5, 865 - 867 (2008)
Published online: 1 August 2008 | doi:10.1038/nmeth.1249
Caenorhabditis elegans mutant allele identification by whole-genome sequencing
Sumeet Sarin1, Snehit Prabhu2, M Maggie O'Meara1, Itsik Pe'er2 & Oliver Hobert1
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
- Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, 701 W. 168th Street, New York, New York 10032, USA.
- Department of Computer Science, Columbia University, 1214 Amsterdeam Avenue, New York, New York 10027, USA.
Correspondence to: Itsik Pe'er2 e-mail: ip2169@columbia.edu
Correspondence to: Oliver Hobert1 e-mail: or38@columbia.edu
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