Brief Communication abstract
Nature Methods 5, 869 - 872 (2008)
Published online: 31 August 2008 | doi:10.1038/nmeth.1250
Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans
Maria Doitsidou1, Nuria Flames1, Albert C Lee1, Alexander Boyanov1 & Oliver Hobert1
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately.
- Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, 701 W. 168th Street, New York, New York 10032, USA.
Correspondence to: Maria Doitsidou1 e-mail: md2398@columbia.edu
Correspondence to: Oliver Hobert1 e-mail: or38@columbia.edu
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