Article abstract
Nature Methods 5, 881 - 886 (2008)
Published online: 21 September 2008 | Corrected online: 29 September 2008 | doi:10.1038/nmeth.1255
There is a Erratum (November 2008) associated with this Article.
Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering
Marco Cammarata1,2, Matteo Levantino3, Friedrich Schotte4, Philip A Anfinrud4, Friederike Ewald1, Jungkweon Choi5, Antonio Cupane3, Michael Wulff1 & Hyotcherl Ihee5
Abstract
We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights into the structural dynamics of proteins functioning in their natural environment, TR-WAXS complements and extends results obtained with time-resolved optical spectroscopy and X-ray crystallography.
- European Synchrotron Radiation Facility, Grenoble Cedex 38043, BP 220, France.
- Centre for Molecular Movies, Niels Bohr Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark.
- Department of Physical and Astronomical Sciences, University of Palermo, via Archirafi 36, I-90123 Palermo, Italy.
- Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 31 Center Drive, Bethesda, Maryland 20892, USA.
- Center for Time-Resolved Diffraction, Department of Chemistry (BK21), Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Correspondence to: Marco Cammarata1,2 e-mail: marco.cammarata@esrf.eu
Correspondence to: Hyotcherl Ihee5 e-mail: hyotcherl.ihee@kaist.ac.kr
s. Additionally, the time delay of 320 ms reported in Figure 5b is incorrect. The correct time delay is 200 ms. These errors have been corrected in the HTML and PDF versions of the article.MORE ARTICLES LIKE THIS
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