Brief Communication abstract
Nature Methods 5, 1019 - 1021 (2008)
Published online: 9 November 2008 | doi:10.1038/nmeth.1269
Intravital imaging of metastatic behavior through a mammary imaging window
Dmitriy Kedrin1,4, Bojana Gligorijevic1,2,4, Jeffrey Wyckoff1,2, Vladislav V Verkhusha1,2, John Condeelis1,2, Jeffrey E Segall1 & Jacco van Rheenen1,2,3
We report a technique to evaluate the same tumor microenvironment over multiple intravital imaging sessions in living mice. We optically marked individual tumor cells expressing photoswitchable proteins in an orthotopic mammary carcinoma and followed them for extended periods through a mammary imaging window. We found that two distinct microenvironments in the same orthotopic mammary tumor affected differently the invasion and intravasation of tumor cells.
- Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
- Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
- Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.
- These authors contributed equally to this work.
Correspondence to: Jacco van Rheenen1,2,3 e-mail: j.vanrheenen@niob.knaw.nl
Correspondence to: John Condeelis1,2 e-mail: condeeli@aecom.yu.edu
Correspondence to: Jeffrey E Segall1 e-mail: segall@aecom.yu.edu
