Brief Communication abstract
Nature Methods 5, 1027 - 1030 (2008)
Published online: 16 November 2008 | doi:10.1038/nmeth.1271
Nanoscale imaging of molecular positions and anisotropies
Travis J Gould1,5, Mudalige S Gunewardene1,5, Manasa V Gudheti1, Vladislav V Verkhusha2, Shu-Rong Yin3, Julie A Gosse4 & Samuel T Hess1
Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
- Department of Physics and Astronomy and Institute for Molecular Biophysics, 5709 Bennett Hall, University of Maine, Orono, Maine 04469, USA.
- Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
- Laboratory for Cellular and Molecular Biophysics, Program in Physical Biology, National Institute of Child Health and Human Development, 10 Center Drive, Bethesda, Maryland 20892, USA.
- Department of Biochemistry, Microbiology, and Molecular Biology, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469, USA.
- These authors contributed equally to this work.
Correspondence to: Samuel T Hess1 e-mail: sam.hess@umit.maine.edu
