Advance online publication
The latest research papers, published online ahead of print. These online versions are definitive and may be cited using the digital object identifier (DOI).
About advance online publicationArticle
High-resolution mapping of copy-number alterations with massively parallel sequencing
Derek Y Chiang, Gad Getz, David B Jaffe, Michael J T O'Kelly, Xiaojun Zhao, Scott L Carter, Carsten Russ, Chad Nusbaum, Matthew Meyerson & Eric S Lander
Published online: 30 November 2008; | doi:10.1038/nmeth.1276
Massively parallel sequencing is a precise way to analyze copy-number variations given the right computational tools. An algorithm now facilitates the detection and fine mapping of copy-number gains and losses from millions of short sequence reads.
Abstract - | Full Text - High-resolution mapping of copy-number alterations with massively parallel sequencing | PDF (1,441 KB) - High-resolution mapping of copy-number alterations with massively parallel sequencing | Supplementary information
Brief Communication
Stable knockdown of microRNA in vivo by lentiviral vectors
Bernhard Gentner, Giulia Schira, Alice Giustacchini, Mario Amendola, Brian D Brown, Maurilio Ponzoni & Luigi Naldini
Published online: 30 November 2008; | doi:10.1038/nmeth.1277
To study microRNA function in vivo, the authors optimize lentiviral-driven expression of microRNA target sequences in mice and show dose-dependent inhibition of microRNA-mediated regulation of reporter constructs as well as of natural microRNA targets. With the inhibition of a miR-223, they can phenocopy the knockout of this microRNA.
Abstract - | Full Text - Stable knockdown of microRNA in vivo by lentiviral vectors | PDF (356 KB) - Stable knockdown of microRNA in vivo by lentiviral vectors | Supplementary information
Until print versions of AOP papers are published, they should be cited in the style "Author(s) Nature Methods advance online publication, day month year (doi:10.1038/nmethXXXXX)". Once the print version (identical to the AOP) is published, it should be cited as follows: "Author(s) Nature Methods volume, page (year); advance online publication, (doi:10.1038/nmethXXXXX)".
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