Top 10
The top ten charts the articles that have been downloaded most often, in PDF format, from the Nature Methods website in recent weeks. The chart does not rank the quality, scientific significance or citation impact of the content. However, it may bring articles to your attention that you might not have otherwise noticed. We hope you enjoy them.
Top of pageTechnology Feature
Mass spectrometry and proteomics: hitting the mark
Nathan Blow
doi:10.1038/nmeth0808-741
Abstract - | Full Text - Mass spectrometry and proteomics: hitting the mark | PDF (483 KB) - Mass spectrometry and proteomics: hitting the mark
Article
Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data
Anton Valouev, David S Johnson, Andreas Sundquist, Catherine Medina, Elizabeth Anton, Serafim Batzoglou, Richard M Myers & Arend Sidow
doi:10.1038/nmeth.1246
Abstract - Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data | Full Text - Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data | PDF (349 KB) - Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data | Supplementary information - Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data
Article
Mapping and quantifying mammalian transcriptomes by RNA-Seq
Ali Mortazavi, Brian A Williams, Kenneth McCue, Lorian Schaeffer & Barbara Wold
doi:10.1038/nmeth.1226
Abstract - Mapping and quantifying mammalian transcriptomes by RNA-Seq | Full Text - Mapping and quantifying mammalian transcriptomes by RNA-Seq | PDF (648 KB) - Mapping and quantifying mammalian transcriptomes by RNA-Seq | Supplementary information - Mapping and quantifying mammalian transcriptomes by RNA-Seq
See also: News and Views by Shendure
Review
Protein production and purification
Structural Genomics Consortium, Architecture et Fonction des Macromolécules Biologiques, Berkeley Structural Genomics Center, China Structural Genomics Consortium, Integrated Center for Structure and Function Innovation, Israel Structural Proteomics Center, Joint Center for Structural Genomics, Midwest Center for Structural Genomics, New York Structural GenomiX Research Center for Structural Genomics, Northeast Structural Genomics Consortium, Oxford Protein Production Facility, Protein Sample Production Facility, Max Delbrück Center for Molecular Medicine, RIKEN Structural Genomics/Proteomics Initiative & SPINE2-Complexes
doi:10.1038/nmeth.f.202
Abstract - Protein production and purification | Full Text - Protein production and purification | PDF (311 KB) - Protein production and purification | Supplementary information - Protein production and purification
Research Highlights
A time stamp for proteins
Daniel Evanko
doi:10.1038/nmeth0808-662a
Abstract - | Full Text - A time stamp for proteins | PDF (202 KB) - A time stamp for proteins
News and Views
Single-particle tracking: connecting the dots
Michael J Saxton
doi:10.1038/nmeth0808-671
Abstract - | Full Text - Single-particle tracking: connecting the dots | PDF (128 KB) - Single-particle tracking: connecting the dots
See also: [could not find see also article id nmeth.1233! Check Log.] | [could not find see also article id nmeth.1237! Check Log.]
Article
Stem cell transcriptome profiling via massive-scale mRNA sequencing
Nicole Cloonan, Alistair R R Forrest, Gabriel Kolle, Brooke B A Gardiner, Geoffrey J Faulkner, Mellissa K Brown, Darrin F Taylor, Anita L Steptoe, Shivangi Wani, Graeme Bethel, Alan J Robertson, Andrew C Perkins, Stephen J Bruce, Clarence C Lee, Swati S Ranade, Heather E Peckham, Jonathan M Manning, Kevin J McKernan & Sean M Grimmond
doi:10.1038/nmeth.1223
Abstract - Stem cell transcriptome profiling via massive-scale mRNA sequencing | Full Text - Stem cell transcriptome profiling via massive-scale mRNA sequencing | PDF (489 KB) - Stem cell transcriptome profiling via massive-scale mRNA sequencing | Supplementary information - Stem cell transcriptome profiling via massive-scale mRNA sequencing
See also: News and Views by Shendure
Brief Communication
Caenorhabditis elegans mutant allele identification by whole-genome sequencing
Sumeet Sarin, Snehit Prabhu, M Maggie O'Meara, Itsik Pe'er & Oliver Hobert
doi:10.1038/nmeth.1249
Abstract - Caenorhabditis elegans mutant allele identification by whole-genome sequencing | Full Text - Caenorhabditis elegans mutant allele identification by whole-genome sequencing | PDF (225 KB) - Caenorhabditis elegans mutant allele identification by whole-genome sequencing | Supplementary information - Caenorhabditis elegans mutant allele identification by whole-genome sequencing
See also: News and Views by Fay
Article
A genetically encoded calcium indicator for chronic in vivo two-photon imaging
Marco Mank, Alexandre Ferrão Santos, Stephan Direnberger, Thomas D Mrsic-Flogel, Sonja B Hofer, Valentin Stein, Thomas Hendel, Dierk F Reiff, Christiaan Levelt, Alexander Borst, Tobias Bonhoeffer, Mark Hübener & Oliver Griesbeck
doi:10.1038/nmeth.1243
Abstract - A genetically encoded calcium indicator for chronic in vivo two-photon imaging | Full Text - A genetically encoded calcium indicator for chronic in vivo two-photon imaging | PDF (516 KB) - A genetically encoded calcium indicator for chronic in vivo two-photon imaging | Supplementary information - A genetically encoded calcium indicator for chronic in vivo two-photon imaging