Nature Protocols Nature Protocols is an interactive online resource for laboratory protocols for bench researchers. The core content is high quality, peer-reviewed protocols that are presented in a 'recipe' style, providing step-by-step descriptions of procedures that users can take to the lab bench and immediately apply in their own research. Nature Protocols aims to publish the protocols being used to answer outstanding biological and biomedical science research questions, including methods grounded in physics and chemistry that have a practical application to the study of biological problems. http://www.nature.com/nprot/current_issue/ Nature Publishing Group en © 2009 Nature Publishing Group Nature Protocols 1750-2799 1750-2799 © 2009 Nature Publishing Group permissions@nature.com Nature Protocols http://www.nature.com/includes/rj_globnavimages/nprot_logo.gif http://www.nature.com/nprot/ Voltage-clamp and current-clamp recordings from mammalian DRG neurons http://dx.doi.org/10.1038/nprot.2009.91 We provide here detailed electrophysiological protocols to study voltage-gated sodium channels and to investigate how wild-type and mutant channels influence firing properties of transfected mammalian dorsal root ganglion (DRG) neurons. Whole-cell voltage-clamp recordings permit us to analyze kinetic and voltage-dependence properties of ion channels and Voltage-clamp and current-clamp recordings from mammalian DRG neurons

Nature Protocols 4, 1103 (2009). doi:10.1038/nprot.2009.91

Authors: Theodore R Cummins, Anthony M Rush, Mark Estacion, Sulayman D Dib-Hajj & Stephen G Waxman

We provide here detailed electrophysiological protocols to study voltage-gated sodium channels and to investigate how wild-type and mutant channels influence firing properties of transfected mammalian dorsal root ganglion (DRG) neurons. Whole-cell voltage-clamp recordings permit us to analyze kinetic and voltage-dependence properties of ion channels and

]]> Voltage-clamp and current-clamp recordings from mammalian DRG neurons Theodore R Cummins Anthony M Rush Mark Estacion Sulayman D Dib-Hajj Stephen G Waxman doi:10.1038/nprot.2009.91 Nature Protocols 4, 1103 (2009) 2009-07-09 Nature Protocols 2009-07-09 10.1038/nprot.2009.91 http://dx.doi.org/10.1038/nprot.2009.91 4 8 Protocol 1103 1112 Synthesis of bicyclo[5.3.0]azulene derivatives http://dx.doi.org/10.1038/nprot.2009.99 Azulene has been recognized for its application in medicinal therapy against inflammation. Recently, azulene analogs have been used in optical technology. Nevertheless, synthesis of this family of compounds is always associated with multiple challenges. In this protocol, we describe a time-efficient and cost-effective procedure for Synthesis of bicyclo[5.3.0]azulene derivatives

Nature Protocols 4, 1113 (2009). doi:10.1038/nprot.2009.99

Authors: Donald D Nolting, Michael Nickels, Ronald Price, John C Gore & Wellington Pham

Azulene has been recognized for its application in medicinal therapy against inflammation. Recently, azulene analogs have been used in optical technology. Nevertheless, synthesis of this family of compounds is always associated with multiple challenges. In this protocol, we describe a time-efficient and cost-effective procedure for

]]> Synthesis of bicyclo[5.3.0]azulene derivatives Donald D Nolting Michael Nickels Ronald Price John C Gore Wellington Pham doi:10.1038/nprot.2009.99 Nature Protocols 4, 1113 (2009) 2009-07-09 Nature Protocols 2009-07-09 10.1038/nprot.2009.99 http://dx.doi.org/10.1038/nprot.2009.99 4 8 Protocol 1113 1117 Transfection of rat or mouse neurons by biolistics or electroporation http://dx.doi.org/10.1038/nprot.2009.90 Properties of ion channels are affected by the background of the cells in which they are expressed. Thus, it is important for investigators interested in neuronal function to study these proteins in post-mitotic neurons. However, post-mitotic neurons, and many cell lines, are difficult to transfect Transfection of rat or mouse neurons by biolistics or electroporation

Nature Protocols 4, 1118 (2009). doi:10.1038/nprot.2009.90

Authors: Sulayman D Dib-Hajj, Jin Sung Choi, Lawrence J Macala, Lynda Tyrrell, Joel A Black, Theodore R Cummins & Stephen G Waxman

Properties of ion channels are affected by the background of the cells in which they are expressed. Thus, it is important for investigators interested in neuronal function to study these proteins in post-mitotic neurons. However, post-mitotic neurons, and many cell lines, are difficult to transfect

]]> Transfection of rat or mouse neurons by biolistics or electroporation Sulayman D Dib-Hajj Jin Sung Choi Lawrence J Macala Lynda Tyrrell Joel A Black Theodore R Cummins Stephen G Waxman doi:10.1038/nprot.2009.90 Nature Protocols 4, 1118 (2009) 2009-07-09 Nature Protocols 2009-07-09 10.1038/nprot.2009.90 http://dx.doi.org/10.1038/nprot.2009.90 4 8 Protocol 1118 1126