Synthesis of N-succinimidyl 4-[¹⁸F]fluorobenzoate, an agent for labeling proteins and peptides with ¹⁸F - pp1655 - 1661

Ganesan Vaidyanathan & Michael R Zalutsky

Published online: 09 November 2006 | doi:10.1038/nprot.2006.264

Abstract - | Full Text - Synthesis of N-succinimidyl 4-[18F]fluorobenzoate, an agent for labeling proteins and peptides with 18F | PDF (189 KB) - Synthesis of N-succinimidyl 4-[18F]fluorobenzoate, an agent for labeling proteins and peptides with 18F

Drug-induced conditioned place preference and aversion in mice - pp1662 - 1670

Christopher L Cunningham, Christina M Gremel & Peter A Groblewski

Published online: 16 November 2006 | doi:10.1038/nprot.2006.279

Abstract - | Full Text - Drug-induced conditioned place preference and aversion in mice | PDF (282 KB) - Drug-induced conditioned place preference and aversion in mice

See also: Protocol by Alamed et al. | Protocol by Stromnes & Goverman | Protocol by Stromnes & Goverman

Two-day radial-arm water maze learning and memory task; robust resolution of amyloid-related memory deficits in transgenic mice - pp1671 - 1679

Jennifer Alamed, Donna M Wilcock, David M Diamond, Marcia N Gordon & Dave Morgan

Published online: 09 November 2006 | doi:10.1038/nprot.2006.275

Abstract - | Full Text - Two-day radial-arm water maze learning and memory task; robust resolution of amyloid-related memory deficits in transgenic mice | PDF (310 KB) - Two-day radial-arm water maze learning and memory task; robust resolution of amyloid-related memory deficits in transgenic mice | Supplementary information

See also: Protocol by Cunningham et al. | Protocol by Stromnes & Goverman | Protocol by Stromnes & Goverman

Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores - pp1680 - 1697

Hugh Dove & Robert W Mayes

Published online: 22 November 2006 | doi:10.1038/nprot.2006.225

Abstract - | Full Text - Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores | PDF (414 KB) - Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores | Supplementary information

Synthesis of cationic conjugated polymers for use in label-free DNA microarrays - pp1698 - 1702

Bin Liu & Guillermo C Bazan

Published online: 16 November 2006 | doi:10.1038/nprot.2006.272

Abstract - | Full Text - Synthesis of cationic conjugated polymers for use in label-free DNA microarrays | PDF (174 KB) - Synthesis of cationic conjugated polymers for use in label-free DNA microarrays

High-throughput transgenesis in Xenopus using I-SceI meganuclease - pp1703 - 1710

Hajime Ogino, William B McConnell & Robert M Grainger

Published online: 16 November 2006 | doi:10.1038/nprot.2006.208

Abstract - | Full Text - High-throughput transgenesis in Xenopus using I-SceI meganuclease | PDF (256 KB) - High-throughput transgenesis in Xenopus using I-SceI meganuclease

Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species - pp1711 - 1724

Fernando Patolsky, Gengfeng Zheng & Charles M Lieber

Published online: 16 November 2006 | doi:10.1038/nprot.2006.227

Abstract - | Full Text - Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species | PDF (1,261 KB) - Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species

Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and acridine orange in Drosophila embryos and adult male gonads - pp1725 - 1731

Eli Arama & Hermann Steller

Published online: 16 November 2006 | doi:10.1038/nprot.2006.235

Abstract - | Full Text - Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and acridine orange in Drosophila embryos and adult male gonads | PDF (279 KB) - Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and acridine orange in Drosophila embryos and adult male gonads

Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling - pp1732 - 1742

Nilesh S Tannu & Scott E Hemby

Published online: 16 November 2006 | doi:10.1038/nprot.2006.256

Abstract - | Full Text - Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling | PDF (575 KB) - Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling

See also: Protocol by Sadowski et al.

Serial analysis of gene expression - pp1743 - 1760

Min Hu & Kornelia Polyak

Published online: 16 November 2006 | doi:10.1038/nprot.2006.269

Abstract - | Full Text - Serial analysis of gene expression | PDF (425 KB) - Serial analysis of gene expression

Single-nucleotide polymorphisms: analysis by mass spectrometry - pp1761 - 1771

Sascha Sauer, Richard Reinhardt, Hans Lehrach & Ivo G Gut

Published online: 22 November 2006 | doi:10.1038/nprot.2006.257

Abstract - | Full Text - Single-nucleotide polymorphisms: analysis by mass spectrometry | PDF (335 KB) - Single-nucleotide polymorphisms: analysis by mass spectrometry

Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assay - pp1772 - 1777

Timothy R Mahoney, Shuo Luo & Michael L Nonet

Published online: 16 November 2006 | doi:10.1038/nprot.2006.281

Abstract - | Full Text - Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assay | PDF (242 KB) - Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assay | Supplementary information

Quantitative proteomic approach to study subcellular localization of membrane proteins - pp1778 - 1789

Pawel G Sadowski, Tom PJ Dunkley, Ian P Shadforth, Paul Dupree, Conrad Bessant, Julian L Griffin & Kathryn S Lilley

Published online: 16 November 2006 | doi:10.1038/nprot.2006.254

Abstract - | Full Text - Quantitative proteomic approach to study subcellular localization of membrane proteins | PDF (382 KB) - Quantitative proteomic approach to study subcellular localization of membrane proteins | Supplementary information

See also: Protocol by Tannu & Hemby

Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization - pp1790 - 1798

Lucy McDonald & Robert J Beynon

Published online: 16 November 2006 | doi:10.1038/nprot.2006.317

Abstract - | Full Text - Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization | PDF (495 KB) - Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization

See also: Protocol by Cox & Emili

Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions - pp1799 - 1809

Cristiane del Corsso, Miduturu Srinivas, Marcia Urban-Maldonado, Alonso P Moreno, Alfredo G Fort, Glenn I Fishman & David C Spray

Published online: 16 November 2006 | doi:10.1038/nprot.2006.266

Abstract - | Full Text - Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions | PDF (664 KB) - Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions

Active induction of experimental allergic encephalomyelitis - pp1810 - 1819

Ingunn M Stromnes & Joan M Goverman

Published online: 16 November 2006 | doi:10.1038/nprot.2006.285

Abstract - | Full Text - Active induction of experimental allergic encephalomyelitis | PDF (273 KB) - Active induction of experimental allergic encephalomyelitis

See also: Protocol by Cunningham et al. | Protocol by Stromnes & Goverman | Protocol by Alamed et al.

pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli - pp1820 - 1827

Prabhat Kumar Purbey, P Cyril Jayakumar, Milind S Patole & Sanjeev Galande

Published online: 16 November 2006 | doi:10.1038/nprot.2006.310

Abstract - | Full Text - pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli | PDF (282 KB) - pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli

Repeated measures of shoaling tendency in zebrafish (Danio rerio) and other small teleost fishes - pp1828 - 1831

Dominic Wright & Jens Krause

Published online: 16 November 2006 | doi:10.1038/nprot.2006.287

Abstract - | Full Text - Repeated measures of shoaling tendency in zebrafish (Danio rerio) and other small teleost fishes | PDF (248 KB) - Repeated measures of shoaling tendency in zebrafish (Danio rerio) and other small teleost fishes

Selecting effective siRNAs based on guide RNA structure - pp1832 - 1839

Christian Köberle, Stefan H E Kaufmann & Volker Patzel

Published online: 16 November 2006 | doi:10.1038/nprot.2006.206

Abstract - | Full Text - Selecting effective siRNAs based on guide RNA structure | PDF (438 KB) - Selecting effective siRNAs based on guide RNA structure

Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative estimation by flow cytometry - pp1840 - 1851

Srinivasa-Gopalan Sampathkumar, Mark B Jones & Kevin J Yarema

Published online: 16 November 2006 | doi:10.1038/nprot.2006.252

Abstract - | Full Text - Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative estimation by flow cytometry | PDF (539 KB) - Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative estimation by flow cytometry

Silver staining of proteins in polyacrylamide gels - pp1852 - 1858

Mireille Chevallet, Sylvie Luche & Thierry Rabilloud

Published online: 22 November 2006 | doi:10.1038/nprot.2006.288

Abstract - | Full Text - Silver staining of proteins in polyacrylamide gels | PDF (287 KB) - Silver staining of proteins in polyacrylamide gels

Simultaneous imaging of morphological plasticity and calcium dynamics in dendrites - pp1859 - 1864

Susanne B Lang, Tobias Bonhoeffer & Christian Lohmann

Published online: 22 November 2006 | doi:10.1038/nprot.2006.267

Abstract - | Full Text - Simultaneous imaging of morphological plasticity and calcium dynamics in dendrites | PDF (301 KB) - Simultaneous imaging of morphological plasticity and calcium dynamics in dendrites

In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP) - pp1865 - 1871

Huimin Zhao & Wenjuan Zha

Published online: 22 November 2006 | doi:10.1038/nprot.2006.309

Abstract - | Full Text - In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP) | PDF (209 KB) - In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP)

Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics - pp1872 - 1878

Brian Cox & Andrew Emili

Published online: 22 November 2006 | doi:10.1038/nprot.2006.273

Abstract - | Full Text - Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics | PDF (229 KB) - Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics

See also: Protocol by McDonald & Beynon

Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases - pp1879 - 1886

Christian Dalhoff, Gravydas Lukinaviius, Saulius Klimaauskas & Elmar Weinhold

Published online: 22 November 2006 | doi:10.1038/nprot.2006.253

Abstract - | Full Text - Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases | PDF (291 KB) - Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases

Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons - pp1887 - 1897

Edith M Schneider Gasser, Carolin J Straub, Patrizia Panzanelli, Oliver Weinmann, Marco Sassoè-Pognetto & Jean-Marc Fritschy

Published online: 22 November 2006 | doi:10.1038/nprot.2006.265

Abstract - | Full Text - Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons | PDF (607 KB) - Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons

Behavioral testing and preliminary analysis of the hamster visual system - pp1898 - 1905

G E Schneider, R G Ellis-Behnke, Y X Liang, P W F Kau, D K C Tay & K F So

Published online: 22 November 2006 | doi:10.1038/nprot.2006.240

Abstract - | Full Text - Behavioral testing and preliminary analysis of the hamster visual system | PDF (436 KB) - Behavioral testing and preliminary analysis of the hamster visual system | Supplementary information

High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans - pp1906 - 1914

Andrew R Burns, Trevor C Y Kwok, Al Howard, Ed Houston, Karl Johanson, Anthony Chan, Sean R Cutler, Peter McCourt & Peter J Roy

Published online: 22 November 2006 | doi:10.1038/nprot.2006.283

Abstract - | Full Text - High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans | PDF (363 KB) - High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans

An ELISA method to measure inhibition of the COX enzymes - pp1915 - 1921

Muriel Cuendet, Andrew D Mesecar, David L DeWitt & John M Pezzuto

Published online: 22 November 2006 | doi:10.1038/nprot.2006.308

Abstract - | Full Text - An ELISA method to measure inhibition of the COX enzymes | PDF (300 KB) - An ELISA method to measure inhibition of the COX enzymes

High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents - pp1922 - 1928

Martin Toussaint & Antonio Conconi

Published online: 22 November 2006 | doi:10.1038/nprot.2006.304

Abstract - | Full Text - High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents | PDF (288 KB) - High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents

Highly selective enrichment of phosphorylated peptides using titanium dioxide - pp1929 - 1935

Tine E Thingholm, Thomas J D Jørgensen, Ole N Jensen & Martin R Larsen

Published online: 22 November 2006 | doi:10.1038/nprot.2006.185

Abstract - | Full Text - Highly selective enrichment of phosphorylated peptides using titanium dioxide | PDF (630 KB) - Highly selective enrichment of phosphorylated peptides using titanium dioxide

The multiple-choice formats (forms F and G) of the Benton Visual Retention Test as a tool to detect age-related memory changes in population-based studies and clinical settings - pp1936 - 1938

Hélène Amieva, Yann Gaestel & Jean-François Dartigues

Published online: 22 November 2006 | doi:10.1038/nprot.2006.302

Abstract - | Full Text - The multiple-choice formats (forms F and G) of the Benton Visual Retention Test as a tool to detect age-related memory changes in population-based studies and clinical settings | PDF (109 KB) - The multiple-choice formats (forms F and G) of the Benton Visual Retention Test as a tool to detect age-related memory changes in population-based studies and clinical settings

In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples - pp1939 - 1946

Jan Hejátko, Ikram Blilou, Philip B Brewer, Jií Friml, Ben Scheres & Eva Benková

Published online: 22 November 2006 | doi:10.1038/nprot.2006.333

Abstract - | Full Text - In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples | PDF (249 KB) - In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples

Isolation of murine microglial cells for RNA analysis or flow cytometry - pp1947 - 1951

Astrid E Cardona, DeRen Huang, Margaret E Sasse & Richard M Ransohoff

Published online: 22 November 2006 | doi:10.1038/nprot.2006.327

Abstract - | Full Text - Isolation of murine microglial cells for RNA analysis or flow cytometry | PDF (264 KB) - Isolation of murine microglial cells for RNA analysis or flow cytometry

Passive induction of experimental allergic encephalomyelitis - pp1952 - 1960

Ingunn M Stromnes & Joan M Goverman

Published online: 22 November 2006 | doi:10.1038/nprot.2006.284

Abstract - | Full Text - Passive induction of experimental allergic encephalomyelitis | PDF (295 KB) - Passive induction of experimental allergic encephalomyelitis

See also: Protocol by Cunningham et al. | Protocol by Alamed et al. | Protocol by Stromnes & Goverman

A high-throughput fluorimetric assay for angiotensin I-converting enzyme - pp1961 - 1964

Sylva L Schwager, Adriana K Carmona & Edward D Sturrock

Published online: 30 November 2006 | doi:10.1038/nprot.2006.305

Abstract - | Full Text - A high-throughput fluorimetric assay for angiotensin I-converting enzyme | PDF (142 KB) - A high-throughput fluorimetric assay for angiotensin I-converting enzyme

See also: Protocol by Carmona et al.

Whole-genome multiple displacement amplification from single cells - pp1965 - 1970

Claudia Spits, Cédric Le Caignec, Martine De Rycke, Lindsey Van Haute, André Van Steirteghem, Inge Liebaers & Karen Sermon

Published online: 30 November 2006 | doi:10.1038/nprot.2006.326

Abstract - | Full Text - Whole-genome multiple displacement amplification from single cells | PDF (182 KB) - Whole-genome multiple displacement amplification from single cells

A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay - pp1971 - 1976

Adriana K Carmona, Sylva L Schwager, Maria A Juliano, Luiz Juliano & Edward D Sturrock

Published online: 30 November 2006 | doi:10.1038/nprot.2006.306

Abstract - | Full Text - A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay | PDF (225 KB) - A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay

See also: Protocol by Schwager et al.

Combining patch-clamping of cells in brain slices with immunocytochemical labeling to define cell type and developmental stage - pp1977 - 1986

Ragnhildur Káradóttir & David Attwell

Published online: 30 November 2006 | doi:10.1038/nprot.2006.261

Abstract - | Full Text - Combining patch-clamping of cells in brain slices with immunocytochemical labeling to define cell type and developmental stage | PDF (544 KB) - Combining patch-clamping of cells in brain slices with immunocytochemical labeling to define cell type and developmental stage

An efficient Mitsunobu protocol for the one-pot synthesis of S-glycosyl amino-acid building blocks and their use in combinatorial spot synthesis of glycopeptide libraries - pp1987 - 1994

Thomas Ziegler & Carsten Schips

Published online: 30 November 2006 | doi:10.1038/nprot.2006.307

Abstract - | Full Text - An efficient Mitsunobu protocol for the one-pot synthesis of S-glycosyl amino-acid building blocks and their use in combinatorial spot synthesis of glycopeptide libraries | PDF (346 KB) - An efficient Mitsunobu protocol for the one-pot synthesis of S-glycosyl amino-acid building blocks and their use in combinatorial spot synthesis of glycopeptide libraries

Quantitative gas chromatography mass spectrometric analysis of 2'-deoxyinosine in tissue DNA - pp1995 - 2002

Kok Seong Lim, Andrew Jenner & Barry Halliwell

Published online: 30 November 2006 | doi:10.1038/nprot.2006.301

Abstract - | Full Text - Quantitative gas chromatography mass spectrometric analysis of 2'-deoxyinosine in tissue DNA | PDF (311 KB) - Quantitative gas chromatography mass spectrometric analysis of 2'-deoxyinosine in tissue DNA

Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A - pp2003 - 2011

Jeffrey T Mason, Lixin Xu, Zong-mei Sheng, Junkun He & Timothy J O'Leary

Published online: 30 November 2006 | doi:10.1038/nprot.2006.331

Abstract - | Full Text - Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A | PDF (276 KB) - Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A

Development of a highly reproducible three-dimensional organotypic model of the oral mucosa - pp2012 - 2018

Anna Dongari-Bagtzoglou & Helena Kashleva

Published online: 30 November 2006 | doi:10.1038/nprot.2006.323

Abstract - | Full Text - Development of a highly reproducible three-dimensional organotypic model of the oral mucosa | PDF (1,939 KB) - Development of a highly reproducible three-dimensional organotypic model of the oral mucosa

Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants - pp2019 - 2025

Imogen A Sparkes, John Runions, Anne Kearns & Chris Hawes

Published online: 30 November 2006 | doi:10.1038/nprot.2006.286

Abstract - | Full Text - Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants | PDF (401 KB) - Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants

In vitro generation of germ cells from murine embryonic stem cells - pp2026 - 2036

Jason A West, In-Hyun Park, George Q Daley & Niels Geijsen

Published online: 30 November 2006 | doi:10.1038/nprot.2006.303

Abstract - | Full Text - In vitro generation of germ cells from murine embryonic stem cells | PDF (339 KB) - In vitro generation of germ cells from murine embryonic stem cells

Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch-specific Surveyor Nuclease - pp2037 - 2047

Sylvie Bannwarth, Vincent Procaccio & Veronique Paquis-Flucklinger

Published online: 30 November 2006 | doi:10.1038/nprot.2006.318

Abstract - | Full Text - Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch-specific Surveyor Nuclease | PDF (380 KB) - Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch-specific Surveyor Nuclease

Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment - pp2048 - 2060

Tove Olafsen, Vania E Kenanova & Anna M Wu

Published online: 30 November 2006 | doi:10.1038/nprot.2006.322

Abstract - | Full Text - Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment | PDF (392 KB) - Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment

Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase - pp2061 - 2067

Eduardo Busto, Vicente Gotor-Fernández & Vicente Gotor

Published online: 30 November 2006 | doi:10.1038/nprot.2006.335

Abstract - | Full Text - Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase | PDF (218 KB) - Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase

Peterson olefination from -silyl aldehydes - pp2068 - 2074

Francisco J Pulido & Asunción Barbero

Published online: 30 November 2006 | doi:10.1038/nprot.2006.321

Abstract - | Full Text - Peterson olefination from -silyl aldehydes | PDF (171 KB) - Peterson olefination from -silyl aldehydes

Patch-clamp recording from mossy fiber terminals in hippocampal slices - pp2075 - 2081

Josef Bischofberger, Dominique Engel, Liyi Li, Jörg RP Geiger & Peter Jonas

Published online: 30 November 2006 | doi:10.1038/nprot.2006.312

Abstract - | Full Text - Patch-clamp recording from mossy fiber terminals in hippocampal slices | PDF (538 KB) - Patch-clamp recording from mossy fiber terminals in hippocampal slices | Supplementary information

Derivation of mouse embryonic stem cells - pp2082 - 2087

Vítzslav Bryja, Sonia Bonilla & Ernest Arenas

Published online: 30 November 2006 | doi:10.1038/nprot.2006.355

Abstract - | Full Text - Derivation of mouse embryonic stem cells | PDF (323 KB) - Derivation of mouse embryonic stem cells

Inexpensive low-oxygen incubators - pp2088 - 2090

Woodring E Wright & Jerry W Shay

Published online: 07 December 2006 | doi:10.1038/nprot.2006.374

Abstract - | Full Text - Inexpensive low-oxygen incubators | PDF (210 KB) - Inexpensive low-oxygen incubators

Probing 2-dimensional protein–protein interactions on model membranes - pp2091 - 2103

Martynas Gavutis, Suman Lata & Jacob Piehler

Published online: 07 December 2006 | doi:10.1038/nprot.2006.270

Abstract - | Full Text - Probing 2-dimensional protein–protein interactions on model membranes | PDF (888 KB) - Probing 2-dimensional protein–protein interactions on model membranes

See also: Protocol by Lata & Piehler

Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes - pp2104 - 2109

Suman Lata & Jacob Piehler

Published online: 07 December 2006 | doi:10.1038/nprot.2006.271

Abstract - | Full Text - Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes | PDF (145 KB) - Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes

See also: Protocol by Gavutis et al.

A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining - pp2110 - 2115

Joy S Wu & Liqun Luo

Published online: 07 December 2006 | doi:10.1038/nprot.2006.336

Abstract - | Full Text - A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining | PDF (241 KB) - A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining

Real-time assessment of three-dimensional cell aggregation in rotating wall vessel bioreactors in vitro - pp2116 - 2127

Gregory P Botta, Prakash Manley, Steven Miller & Peter I Lelkes

Published online: 14 December 2006 | doi:10.1038/nprot.2006.311

Abstract - | Full Text - Real-time assessment of three-dimensional cell aggregation in rotating wall vessel bioreactors in vitro | PDF (518 KB) - Real-time assessment of three-dimensional cell aggregation in rotating wall vessel bioreactors in vitro

Microfluidic culture platform for neuroscience research - pp2128 - 2136

Jeong Won Park, Behrad Vahidi, Anne M Taylor, Seog Woo Rhee & Noo Li Jeon

Published online: 07 December 2006 | doi:10.1038/nprot.2006.316

Abstract - | Full Text - Microfluidic culture platform for neuroscience research | PDF (476 KB) - Microfluidic culture platform for neuroscience research

Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro - pp2137 - 2142

Shahriar S Yaghoubi & Sanjiv S Gambhir

Published online: 07 December 2006 | doi:10.1038/nprot.2006.334

Abstract - | Full Text - Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro | PDF (215 KB) - Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro

Split single-cell RT-PCR analysis of Purkinje cells - pp2143 - 2151

Shigeyuki Esumi, Ryosuke Kaneko, Yoshimi Kawamura & Takeshi Yagi

Published online: 07 December 2006 | doi:10.1038/nprot.2006.343

Abstract - | Full Text - Split single-cell RT-PCR analysis of Purkinje cells | PDF (407 KB) - Split single-cell RT-PCR analysis of Purkinje cells

Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images - pp2152 - 2161

Alfredo Rodriguez, Douglas B Ehlenberger, Patrick R Hof & Susan L Wearne

Published online: 07 December 2006 | doi:10.1038/nprot.2006.313

Abstract - | Full Text - Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images | PDF (504 KB) - Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images

Fluorescent microsphere technique to measure cerebral blood flow in the rat - pp2162 - 2170

G De Visscher, M Haseldonckx & W Flameng

Published online: 07 December 2006 | doi:10.1038/nprot.2006.332

Abstract - | Full Text - Fluorescent microsphere technique to measure cerebral blood flow in the rat | PDF (287 KB) - Fluorescent microsphere technique to measure cerebral blood flow in the rat

Profiling of DNA replication timing in unsynchronized cell populations - pp2171 - 2177

Véronique Azuara

Published online: 07 December 2006 | doi:10.1038/nprot.2006.353

Abstract - | Full Text - Profiling of DNA replication timing in unsynchronized cell populations | PDF (198 KB) - Profiling of DNA replication timing in unsynchronized cell populations

Culture of human mast cells from peripheral blood progenitors - pp2178 - 2183

Hirohisa Saito, Atsushi Kato, Kenji Matsumoto & Yoshimichi Okayama

Published online: 07 December 2006 | doi:10.1038/nprot.2006.344

Abstract - | Full Text - Culture of human mast cells from peripheral blood progenitors | PDF (349 KB) - Culture of human mast cells from peripheral blood progenitors | Supplementary information