Immobilization of enzymes on heterofunctional epoxy supports - pp1022 - 1033

Cesar Mateo, Valeria Grazu, Jose M Palomo, Fernando Lopez-Gallego, Roberto Fernandez-Lafuente & Jose M Guisan

Published online: 26 April 2007 | doi:10.1038/nprot.2007.133

Abstract - Immobilization of enzymes on heterofunctional epoxy supports | Full Text - Immobilization of enzymes on heterofunctional epoxy supports | PDF (718 KB) - Immobilization of enzymes on heterofunctional epoxy supports

Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells - pp1034 - 1043

Miriam Bibel, Jens Richter, Emmanuel Lacroix & Yves-Alain Barde

Published online: 26 April 2007 | doi:10.1038/nprot.2007.147

Abstract - Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells | Full Text - Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells | PDF (525 KB) - Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells

Isolation and culture of rat and mouse oligodendrocyte precursor cells - pp1044 - 1051

Ying Chen, Veerakumar Balasubramaniyan, Jie Peng, Edward C Hurlock, Michelle Tallquist, Jianrong Li & Q Richard Lu

Published online: 26 April 2007 | doi:10.1038/nprot.2007.149

Abstract - Isolation and culture of rat and mouse oligodendrocyte precursor cells | Full Text - Isolation and culture of rat and mouse oligodendrocyte precursor cells | PDF (364 KB) - Isolation and culture of rat and mouse oligodendrocyte precursor cells

Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls - pp1052 - 1058

Steven G Chaulk & Andrew M MacMillan

Published online: 26 April 2007 | doi:10.1038/nprot.2007.154

Abstract - Synthesis of oligo-RNAs with photocaged adenosine 2[prime]-hydroxyls | Full Text - Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls | PDF (212 KB) - Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls

Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis - pp1059 - 1069

Michael Mazanek, Goran Mituloviæ, Franz Herzog, Christoph Stingl, James RA Hutchins, Jan-Michael Peters & Karl Mechtler

Published online: 14 December 2006 | doi:10.1038/nprot.2006.280

Abstract - Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis | Full Text - Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis | PDF (354 KB) - Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis

Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach - pp1070 - 1083

Mire Zloh, Sunil Shaunak, Sibu Balan & Steve Brocchini

Published online: 26 April 2007 | doi:10.1038/nprot.2007.119

Abstract - Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach | Full Text - Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach | PDF (579 KB) - Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach

Cytokinesis-block micronucleus cytome assay - pp1084 - 1104

Michael Fenech

Published online: 03 May 2007 | doi:10.1038/nprot.2007.77

Abstract - Cytokinesis-block micronucleus cytome assay | Full Text - Cytokinesis-block micronucleus cytome assay | PDF (1,190 KB) - Cytokinesis-block micronucleus cytome assay

Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution - pp1105 - 1116

Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando & Yasuhiro Aoyama

Published online: 03 May 2007 | doi:10.1038/nprot.2007.140

Abstract - Cis: -regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution | Full Text - Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution | PDF (428 KB) - Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution

In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction - pp1117 - 1125

Edwin S Levitan, Frederick Lanni & Dinara Shakiryanova

Published online: 03 May 2007 | doi:10.1038/nprot.2007.142

Abstract - In vivo: imaging of vesicle motion and release at the : Drosophila: neuromuscular junction | Full Text - In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction | PDF (454 KB) - In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction | Supplementary information

Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli - pp1126 - 1133

Ali Tavassoli & Stephen J Benkovic

Published online: 03 May 2007 | doi:10.1038/nprot.2007.152

Abstract - Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in : E. coli | Full Text - Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli | PDF (212 KB) - Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli

Development and optimization of high-throughput in vitro protein phosphatase screening assays - pp1134 - 1144

Marni Brisson Tierno, Paul A Johnston, Caleb Foster, John J Skoko, Sunita N Shinde, Tong Ying Shun & John S Lazo

Published online: 03 May 2007 | doi:10.1038/nprot.2007.155

Abstract - Development and optimization of high-throughput : in vitro: protein phosphatase screening assays | Full Text - Development and optimization of high-throughput in vitro protein phosphatase screening assays | PDF (261 KB) - Development and optimization of high-throughput in vitro protein phosphatase screening assays

Tandem affinity purification of functional TAP-tagged proteins from human cells - pp1145 - 1151

Juraj Gregan, Christian G Riedel, Mark Petronczki, Lubos Cipak, Cornelia Rumpf, Ina Poser, Frank Buchholz, Karl Mechtler & Kim Nasmyth

Published online: 03 May 2007 | doi:10.1038/nprot.2007.172

Abstract - Tandem affinity purification of functional TAP-tagged proteins from human cells | Full Text - Tandem affinity purification of functional TAP-tagged proteins from human cells | PDF (169 KB) - Tandem affinity purification of functional TAP-tagged proteins from human cells

Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry - pp1152 - 1165

Yun Xing, Qaiser Chaudry, Christopher Shen, Koon Yin Kong, Haiyen E Zhau, Leland W Chung, John A Petros, Ruth M O'Regan, Maksym V Yezhelyev, Jonathan W Simons, May D Wang & Shuming Nie

Published online: 03 May 2007 | doi:10.1038/nprot.2007.107

Abstract - Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry | Full Text - Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry | PDF (703 KB) - Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry

A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) - pp1166 - 1172

T D Allen, S A Rutherford, S Murray, H S Sanderson, F Gardiner, E Kiseleva, M W Goldberg & S P Drummond

Published online: 10 May 2007 | doi:10.1038/nprot.2007.137

Abstract - A protocol for isolating : Xenopus: oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) | Full Text - A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) | PDF (412 KB) - A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) | Supplementary information

See also: Protocol by Allen et al. | Protocol by Allen et al.

Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM) - pp1173 - 1179

T D Allen, S A Rutherford, S Murray, H S Sanderson, F Gardiner, E Kiseleva, M W Goldberg & S P Drummond

Published online: 10 May 2007 | doi:10.1038/nprot.2007.138

Abstract - Generation of cell-free extracts of : Xenopus: eggs and demembranated sperm chromatin for the assembly and isolation of : in vitro: -formed nuclei for Western blotting and scanning electron microscopy (SEM) | Full Text - Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM) | PDF (345 KB) - Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)

See also: Protocol by Allen et al. | Protocol by Allen et al.

Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells - pp1180 - 1184

T D Allen, S A Rutherford, S Murray, F Gardiner, E Kiseleva, M W Goldberg & S P Drummond

Published online: 10 May 2007 | doi:10.1038/nprot.2007.139

Abstract - Visualization of the nucleus and nuclear envelope : in situ: by SEM in tissue culture cells | Full Text - Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells | PDF (2,151 KB) - Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells | Supplementary information

See also: Protocol by Allen et al. | Protocol by Allen et al.

Imaging mass spectrometry at cellular length scales - pp1185 - 1196

A F Maarten Altelaar, Stefan L Luxembourg, Liam A McDonnell, Sander R Piersma & Ron M A Heeren

Published online: 10 May 2007 | doi:10.1038/nprot.2007.117

Abstract - Imaging mass spectrometry at cellular length scales | Full Text - Imaging mass spectrometry at cellular length scales | PDF (701 KB) - Imaging mass spectrometry at cellular length scales

Selective proliferation of rat hepatocyte progenitor cells in serum-free culture - pp1197 - 1205

Qijie Chen, Junko Kon, Hidekazu Ooe, Kazunori Sasaki & Toshihiro Mitaka

Published online: 10 May 2007 | doi:10.1038/nprot.2007.118

Abstract - Selective proliferation of rat hepatocyte progenitor cells in serum-free culture | Full Text - Selective proliferation of rat hepatocyte progenitor cells in serum-free culture | PDF (349 KB) - Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

Pronuclear injection for the production of transgenic mice - pp1206 - 1215

Lars M Ittner & Jürgen Götz

Published online: 10 May 2007 | doi:10.1038/nprot.2007.145

Abstract - Pronuclear injection for the production of transgenic mice | Full Text - Pronuclear injection for the production of transgenic mice | PDF (521 KB) - Pronuclear injection for the production of transgenic mice

Stripe assay to examine axonal guidance and cell migration - pp1216 - 1224

Bernd Knöll, Christine Weinl, Alfred Nordheim & Friedrich Bonhoeffer

Published online: 10 May 2007 | doi:10.1038/nprot.2007.157

Abstract - Stripe assay to examine axonal guidance and cell migration | Full Text - Stripe assay to examine axonal guidance and cell migration | PDF (445 KB) - Stripe assay to examine axonal guidance and cell migration

Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation - pp1225 - 1235

Mohammad R Seyedsayamdost, Cyril S Yee & JoAnne Stubbe

Published online: 10 May 2007 | doi:10.1038/nprot.2007.159

Abstract - Site-specific incorporation of fluorotyrosines into the R2 subunit of : E. coli: ribonucleotide reductase by expressed protein ligation | Full Text - Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation | PDF (375 KB) - Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation

A protocol for rapid generation of recombinant adenoviruses using the AdEasy system - pp1236 - 1247

Jinyong Luo, Zhong-Liang Deng, Xiaoji Luo, Ni Tang, Wen-Xin Song, Jin Chen, Katie A Sharff, Hue H Luu, Rex C Haydon, Kenneth W Kinzler, Bert Vogelstein & Tong-Chuan He

Published online: 17 May 2007 | doi:10.1038/nprot.2007.135

Abstract - A protocol for rapid generation of recombinant adenoviruses using the AdEasy system | Full Text - A protocol for rapid generation of recombinant adenoviruses using the AdEasy system | PDF (408 KB) - A protocol for rapid generation of recombinant adenoviruses using the AdEasy system

Primary culture of bovine chromaffin cells - pp1248 - 1253

Daniel T O'Connor, Sushil K Mahata, Manjula Mahata, Qijiao Jiang, Vivian Y Hook & Laurent Taupenot

Published online: 17 May 2007 | doi:10.1038/nprot.2007.136

Abstract - Primary culture of bovine chromaffin cells | Full Text - Primary culture of bovine chromaffin cells | PDF (246 KB) - Primary culture of bovine chromaffin cells

Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules - pp1254 - 1262

Matthew P Fletcher, Stephen P Diggle, Miguel Cámara & Paul Williams

Published online: 17 May 2007 | doi:10.1038/nprot.2007.158

Abstract - Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules | Full Text - Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules | PDF (286 KB) - Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules

Affinity purification of plasmid DNA by temperature-triggered precipitation - pp1263 - 1268

U Loi Lao, Jan Kostal, Ashok Mulchandani & Wilfred Chen

Published online: 17 May 2007 | doi:10.1038/nprot.2007.171

Abstract - Affinity purification of plasmid DNA by temperature-triggered precipitation | Full Text - Affinity purification of plasmid DNA by temperature-triggered precipitation | PDF (201 KB) - Affinity purification of plasmid DNA by temperature-triggered precipitation

Collagen-induced arthritis - pp1269 - 1275

David D Brand, Kary A Latham & Edward F Rosloniec

Published online: 17 May 2007 | doi:10.1038/nprot.2007.173

Abstract - Collagen-induced arthritis | Full Text - Collagen-induced arthritis | PDF (262 KB) - Collagen-induced arthritis

Mos1-mediated insertional mutagenesis in Caenorhabditis elegans - pp1276 - 1287

Thomas Boulin & Jean-Louis Bessereau

Published online: 17 May 2007 | doi:10.1038/nprot.2007.192

Abstract - Mos1: -mediated insertional mutagenesis in : Caenorhabditis elegans | Full Text - Mos1-mediated insertional mutagenesis in Caenorhabditis elegans | PDF (909 KB) - Mos1-mediated insertional mutagenesis in Caenorhabditis elegans

A simple one-pot multicomponent synthesis of an octahedral nanocontainer molecule - pp1288 - 1296

Xuejun Liu & Ralf Warmuth

Published online: 17 May 2007 | doi:10.1038/nprot.2007.193

Abstract - A simple one-pot multicomponent synthesis of an octahedral nanocontainer molecule | Full Text - A simple one-pot multicomponent synthesis of an octahedral nanocontainer molecule | PDF (323 KB) - A simple one-pot multicomponent synthesis of an octahedral nanocontainer molecule

Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules - pp1297 - 1305

Sean F Brady

Published online: 17 May 2007 | doi:10.1038/nprot.2007.195

Abstract - Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules | Full Text - Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules | PDF (302 KB) - Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules