Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA - pp1839 - 1848

Jonathan A Stead & Kenneth J McDowall

Published online: 26 July 2007 | doi:10.1038/nprot.2007.248

Abstract - Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA | Full Text - Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA | PDF (431 KB) - Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA

See also: Protocol by Hellman & Fried

Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions - pp1849 - 1861

Lance M Hellman & Michael G Fried

Published online: 26 July 2007 | doi:10.1038/nprot.2007.249

Abstract - Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions | Full Text - Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions | PDF (282 KB) - Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions

See also: Protocol by Stead & McDowall

A fluorescence-based double retrograde tracer strategy for charting central neuronal connections - pp1862 - 1868

Richard Apps & Tom J H Ruigrok

Published online: 26 July 2007 | doi:10.1038/nprot.2007.263

Abstract - A fluorescence-based double retrograde tracer strategy for charting central neuronal connections | Full Text - A fluorescence-based double retrograde tracer strategy for charting central neuronal connections | PDF (222 KB) - A fluorescence-based double retrograde tracer strategy for charting central neuronal connections

See also: Protocol by Ruigrok & Apps

A light microscope-based double retrograde tracer strategy to chart central neuronal connections - pp1869 - 1878

Tom J H Ruigrok & Richard Apps

Published online: 26 July 2007 | doi:10.1038/nprot.2007.264

Abstract - A light microscope-based double retrograde tracer strategy to chart central neuronal connections | Full Text - A light microscope-based double retrograde tracer strategy to chart central neuronal connections | PDF (751 KB) - A light microscope-based double retrograde tracer strategy to chart central neuronal connections

See also: Protocol by Apps & Ruigrok

Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer - pp1879 - 1883

A James Link, Mandy K S Vink & David A Tirrell

Published online: 26 July 2007 | doi:10.1038/nprot.2007.268

Abstract - Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer | Full Text - Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer | PDF (172 KB) - Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer

See also: Protocol by Link et al.

Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor - pp1884 - 1887

A James Link, Mandy K S Vink & David A Tirrell

Published online: 26 July 2007 | doi:10.1038/nprot.2007.269

Abstract - Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor | Full Text - Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor | PDF (140 KB) - Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor

See also: Protocol by Link et al.

The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH₃) and ammonia-free (Li/DBB) conditions - pp1888 - 1895

Timothy J Donohoe & Rhian E Thomas

Published online: 02 August 2007 | doi:10.1038/nprot.2007.245

Abstract - The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH: 3: ) and ammonia-free (Li/DBB) conditions | Full Text - The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions | PDF (371 KB) - The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions

Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips - pp1896 - 1906

Juri Rappsilber, Matthias Mann & Yasushi Ishihama

Published online: 02 August 2007 | doi:10.1038/nprot.2007.261

Abstract - Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips | Full Text - Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips | PDF (355 KB) - Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips

Frontal affinity chromatography—mass spectrometry - pp1907 - 1917

Ella S M Ng, Nora WC Chan, Darren F Lewis, Ole Hindsgaul & David C Schriemer

Published online: 02 August 2007 | doi:10.1038/nprot.2007.262

Abstract - Frontal affinity chromatography[mdash]mass spectrometry | Full Text - Frontal affinity chromatography—mass spectrometry | PDF (361 KB) - Frontal affinity chromatography—mass spectrometry

Detecting antigens by quantitative immuno-PCR - pp1918 - 1930

Christof M Niemeyer, Michael Adler & Ron Wacker

Published online: 02 August 2007 | doi:10.1038/nprot.2007.267

Abstract - Detecting antigens by quantitative immuno-PCR | Full Text - Detecting antigens by quantitative immuno-PCR | PDF (360 KB) - Detecting antigens by quantitative immuno-PCR

Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation - pp1931 - 1936

Mark L Gonzalgo & Gangning Liang

Published online: 02 August 2007 | doi:10.1038/nprot.2007.271

Abstract - Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation | Full Text - Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation | PDF (178 KB) - Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation

Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with N-Boc-imines - pp1937 - 1942

Jung Woon Yang, Michael Stadler & Benjamin List

Published online: 02 August 2007 | doi:10.1038/nprot.2007.272

Abstract - Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with : N: -Boc-imines | Full Text - Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with N-Boc-imines | PDF (225 KB) - Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with N-Boc-imines

A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM) - pp1943 - 1953

Elena Kiseleva, Terry D Allen, Sandra A Rutherford, Steve Murray, Ksenia Morozova, Fiona Gardiner, Martin W Goldberg & Sheona P Drummond

Published online: 09 August 2007 | doi:10.1038/nprot.2007.251

Abstract - A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM) | Full Text - A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM) | PDF (1,182 KB) - A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM)

Site-specific incorporation of nitroxide spin-labels into 2'-positions of nucleic acids - pp1954 - 1962

Thomas E Edwards & Snorri Th Sigurdsson

Published online: 09 August 2007 | doi:10.1038/nprot.2007.273

Abstract - Site-specific incorporation of nitroxide spin-labels into 2[prime]-positions of nucleic acids | Full Text - Site-specific incorporation of nitroxide spin-labels into 2'-positions of nucleic acids | PDF (321 KB) - Site-specific incorporation of nitroxide spin-labels into 2'-positions of nucleic acids

Derivation of human embryonic stem cells from single blastomeres - pp1963 - 1972

Irina Klimanskaya, Young Chung, Sandy Becker, Shi-Jiang Lu & Robert Lanza

Published online: 09 August 2007 | doi:10.1038/nprot.2007.274

Abstract - Derivation of human embryonic stem cells from single blastomeres | Full Text - Derivation of human embryonic stem cells from single blastomeres | PDF (472 KB) - Derivation of human embryonic stem cells from single blastomeres

Digital karyotyping - pp1973 - 1986

Rebecca J Leary, Jordan Cummins, Tian-Li Wang & Victor E Velculescu

Published online: 09 August 2007 | doi:10.1038/nprot.2007.276

Abstract - Digital karyotyping | Full Text - Digital karyotyping | PDF (357 KB) - Digital karyotyping

Characterizing peptides in individual mammalian cells using mass spectrometry - pp1987 - 1997

Stanislav S Rubakhin & Jonathan V Sweedler

Published online: 09 August 2007 | doi:10.1038/nprot.2007.277

Abstract - Characterizing peptides in individual mammalian cells using mass spectrometry | Full Text - Characterizing peptides in individual mammalian cells using mass spectrometry | PDF (551 KB) - Characterizing peptides in individual mammalian cells using mass spectrometry

An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression - pp1998 - 2004

Clemens Neufert, Christoph Becker & Markus F Neurath

Published online: 09 August 2007 | doi:10.1038/nprot.2007.279

Abstract - An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression | Full Text - An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression | PDF (347 KB) - An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression

The detection and quantification of growth cone collapsing activities - pp2005 - 2011

Josef P Kapfhammer, Hong Xu & Jonathan A Raper

Published online: 16 August 2007 | doi:10.1038/nprot.2007.295

Abstract - The detection and quantification of growth cone collapsing activities | Full Text - The detection and quantification of growth cone collapsing activities | PDF (378 KB) - The detection and quantification of growth cone collapsing activities

Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins - pp2012 - 2017

Lan Guan & H Ronald Kaback

Published online: 16 August 2007 | doi:10.1038/nprot.2007.275

Abstract - Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins | Full Text - Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins | PDF (234 KB) - Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins

Synthesis of pyrimidines by direct condensation of amides and nitriles - pp2018 - 2023

Mohammad Movassaghi & Matthew D Hill

Published online: 16 August 2007 | doi:10.1038/nprot.2007.280

Abstract - Synthesis of pyrimidines by direct condensation of amides and nitriles | Full Text - Synthesis of pyrimidines by direct condensation of amides and nitriles | PDF (171 KB) - Synthesis of pyrimidines by direct condensation of amides and nitriles

Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2 - pp2024 - 2032

Dmitriy M Chudakov, Sergey Lukyanov & Konstantin A Lukyanov

Published online: 16 August 2007 | doi:10.1038/nprot.2007.291

Abstract - Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2 | Full Text - Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2 | PDF (340 KB) - Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2

Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays - pp2033 - 2042

Mayka Sanchez, Bruno Galy, Matthias W Hentze & Martina U Muckenthaler

Published online: 16 August 2007 | doi:10.1038/nprot.2007.293

Abstract - Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays | Full Text - Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays | PDF (304 KB) - Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays

Assessing the delivery efficacy and internalization route of cell-penetrating peptides - pp2043 - 2047

Samir El Andaloussi, Peter Guterstam & Ülo Langel

Published online: 16 August 2007 | doi:10.1038/nprot.2007.302

Abstract - Assessing the delivery efficacy and internalization route of cell-penetrating peptides | Full Text - Assessing the delivery efficacy and internalization route of cell-penetrating peptides | PDF (152 KB) - Assessing the delivery efficacy and internalization route of cell-penetrating peptides