Latest Protocols
Volume 4 No 6
- Archive
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Nature protocols
Modeling human listeriosis in natural and genetically engineered animals - pp799 - 810
Olivier Disson, Georgios Nikitas, Solène Grayo, Olivier Dussurget, Pascale Cossart & Marc Lecuit
Published online: 07 May 2009 | doi:10.1038/nprot.2009.66
Abstract - | Full Text - Modeling human listeriosis in natural and genetically engineered animals | PDF (1,040 KB) - Modeling human listeriosis in natural and genetically engineered animals | Supplementary information
Subject Categories: Microbiology and virology | Model organisms
Stepwise differentiation of pluripotent stem cells into retinal cells - pp811 - 824
Fumitaka Osakada, Hanako Ikeda, Yoshiki Sasai & Masayo Takahashi
Published online: 07 May 2009 | doi:10.1038/nprot.2009.51
Abstract - | Full Text - Stepwise differentiation of pluripotent stem cells into retinal cells | PDF (1,102 KB) - Stepwise differentiation of pluripotent stem cells into retinal cells
Subject Category: Cell and tissue culture
Buccal micronucleus cytome assay - pp825 - 837
Philip Thomas, Nina Holland, Claudia Bolognesi, Micheline Kirsch-Volders, Stefano Bonassi, Errol Zeiger, Siegfried Knasmueller & Michael Fenech
Published online: 07 May 2009 | doi:10.1038/nprot.2009.53
Abstract - | Full Text - Buccal micronucleus cytome assay | PDF (613 KB) - Buccal micronucleus cytome assay | Supplementary information
Subject Categories: Detection and probing | Imaging | Spectroscopy
On-wire lithography: synthesis, encoding and biological applications - pp838 - 848
Matthew J Banholzer, Lidong Qin, Jill E Millstone, Kyle D Osberg & Chad A Mirkin
Published online: 14 May 2009 | doi:10.1038/nprot.2009.52
Abstract - | Full Text - On-wire lithography: synthesis, encoding and biological applications | PDF (718 KB) - On-wire lithography: synthesis, encoding and biological applications
Subject Categories: Nanotechnology | Spectroscopy
Microfluidic-assisted analysis of replicating DNA molecules - pp849 - 861
Julia M Sidorova, Nianzhen Li, David C Schwartz, Albert Folch & Raymond J Monnat Jr
Published online: 14 May 2009 | doi:10.1038/nprot.2009.54
Abstract - | Full Text - Microfluidic-assisted analysis of replicating DNA molecules | PDF (572 KB) - Microfluidic-assisted analysis of replicating DNA molecules
Subject Categories: Cell and developmental biology | Genetic analysis | Imaging | Isolation, purification and separation | Nucleic acid based molecular biology
Targeted single-cell electroporation of mammalian neurons in vivo - pp862 - 869
Benjamin Judkewitz, Matteo Rizzi, Kazuo Kitamura & Michael Häusser
Published online: 14 May 2009 | doi:10.1038/nprot.2009.56
Abstract - | Full Text - Targeted single-cell electroporation of mammalian neurons in vivo | PDF (718 KB) - Targeted single-cell electroporation of mammalian neurons in vivo | Supplementary information
Subject Categories: Detection and probing | Neuroscience | Imaging | Model organisms
A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS) - pp870 - 877
Richard D Unwin, John R Griffiths & Anthony D Whetton
Published online: 14 May 2009 | doi:10.1038/nprot.2009.57
Abstract - | Full Text - A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS) | PDF (370 KB) - A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS)
Subject Categories: Biochemistry and protein analysis | Computational and theoretical biology | Genomics and proteomics | Spectroscopy and structural analysis
13C-based metabolic flux analysis - pp878 - 892
Nicola Zamboni, Sarah-Maria Fendt, Martin Rühl & Uwe Sauer
Published online: 21 May 2009 | doi:10.1038/nprot.2009.58
Abstract - | Full Text - 13C-based metabolic flux analysis | PDF (666 KB) - 13C-based metabolic flux analysis | Supplementary information
Subject Categories: In silico modeling | Functional analysis
Construction and flow cytometric screening of targeted enzyme libraries - pp893 - 901
Navin Varadarajan, Jason R Cantor, George Georgiou & Brent L Iverson
Published online: 21 May 2009 | doi:10.1038/nprot.2009.60
Abstract - | Full Text - Construction and flow cytometric screening of targeted enzyme libraries | PDF (393 KB) - Construction and flow cytometric screening of targeted enzyme libraries | Supplementary information
Subject Categories: Biochemistry and protein analysis | Genomics and proteomics | Nucleic acid based molecular biology
Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples - pp902 - 922
Heidi S Erickson, Paul S Albert, John W Gillespie, Jaime Rodriguez-Canales, W Marston Linehan, Peter A Pinto, Rodrigo F Chuaqui & Michael R Emmert-Buck
Published online: 21 May 2009 | doi:10.1038/nprot.2009.61
Abstract - | Full Text - Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples | PDF (1,021 KB) - Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples
Subject Category: Nucleic acid based molecular biology
A coculture assay to visualize and monitor interactions between migrating glioma cells and nerve fibers - pp923 - 927
Patrick Oellers, Maurice Schallenberg, Tobias Stupp, Petar Charalambous, Volker Senner, Werner Paulus & Solon Thanos
Published online: 21 May 2009 | doi:10.1038/nprot.2009.62
Abstract - | Full Text - A coculture assay to visualize and monitor interactions between migrating glioma cells and nerve fibers | PDF (518 KB) - A coculture assay to visualize and monitor interactions between migrating glioma cells and nerve fibers | Supplementary information
Subject Categories: Cell and developmental biology | Cell and tissue culture
Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures - pp928 - 938
Carole Bougault, Anne Paumier, Elisabeth Aubert-Foucher & Frédéric Mallein-Gerin
Published online: 28 May 2009 | doi:10.1038/nprot.2009.63
Abstract - | Full Text - Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures | PDF (445 KB) - Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures
Subject Categories: Biochemistry and protein analysis | Cell and tissue culture | Genetic analysis | Model organisms | Nucleic acid based molecular biology
Stretching polysaccharides on live cells using single molecule force spectroscopy - pp939 - 946
Grégory Francius, David Alsteens, Vincent Dupres, Sarah Lebeer, Sigrid De Keersmaecker, Jos Vanderleyden, Hermann J Gruber & Yves F Dufrêne
Published online: 28 May 2009 | doi:10.1038/nprot.2009.65
Abstract - | Full Text - Stretching polysaccharides on live cells using single molecule force spectroscopy | PDF (754 KB) - Stretching polysaccharides on live cells using single molecule force spectroscopy
Subject Categories: Biochemistry and protein analysis | Cell and developmental biology | Imaging | Microbiology and virology | Nanotechnology | Spectroscopy and structural analysis
A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold - pp947 - 959
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte & Frédéric Dardel
Published online: 28 May 2009 | doi:10.1038/nprot.2009.67
Abstract - | Full Text - A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold | PDF (402 KB) - A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold
Subject Categories: Isolation, purification and separation | Molecular interactions | Recombinant technology
Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing - pp960 - 974
Emily Hodges, Michelle Rooks, Zhenyu Xuan, Arindam Bhattacharjee, D Benjamin Gordon, Leonardo Brizuela, W Richard McCombie & Gregory J Hannon
Published online: 28 May 2009 | doi:10.1038/nprot.2009.68
Abstract - | Full Text - Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing | PDF (607 KB) - Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing
Subject Categories: Genetic analysis | Genomics and proteomics | Nucleic acid based molecular biology
Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos - pp975 - 983
Peter D Vize, Kyle E McCoy & Xiaolan Zhou
Published online: 04 June 2009 | doi:10.1038/nprot.2009.69
Abstract - | Full Text - Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos | PDF (438 KB) - Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos | Supplementary information
Subject Categories: Cell and developmental biology | Imaging | Immunological techniques | Model organisms
Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution - pp984 - 991
Xinrui Duan, Wei Yue, Libing Liu, Zhengping Li, Yuliang Li, Fuchu He, Daoben Zhu, Gangqiao Zhou & Shu Wang
Published online: 04 June 2009 | doi:10.1038/nprot.2009.70
Abstract - | Full Text - Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution | PDF (370 KB) - Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution | Supplementary information
Subject Categories: Genetic analysis | Nucleic acid based molecular biology
Integrated analysis and reconstruction of microbial transcriptional gene regulatory networks using CoryneRegNet - pp992 - 1005
Jan Baumbach, Tobias Wittkop, Christiane Katja Kleindt & Andreas Tauch
Published online: 04 June 2009 | doi:10.1038/nprot.2009.81
Abstract - | Full Text - Integrated analysis and reconstruction of microbial transcriptional gene regulatory networks using CoryneRegNet | PDF (842 KB) - Integrated analysis and reconstruction of microbial transcriptional gene regulatory networks using CoryneRegNet | Supplementary information
Subject Categories: Computational and theoretical biology | Microbiology and virology | Model organisms
