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RBS-ID, a method to identify RNA–protein interactions by crosslinking, uses hydrofluoride to cleave RNA to simple mono-nucleoside adducts, which improves coverage and resolution of RNA binding site identification.
thCHART, a hybridization capture approach using biotinylated LNA-oligonucleotides with toehold architecture, improves the specificity of RNA enrichment and enables detection of the chromosomal spreading pattern of Drosophila roX2 lncRNA under different cellular conditions.