“Borrelia spirochetes activate the innate immune response through a TLR2–MYD88-dependent caspase-1 activation that results in IL-1β-driven joint inflammation”, say Marije Oosting and Leo Joosten, on the basis of their latest research published in Arthritis Research & Therapy.
The investigators generated a new mouse model of Lyme disease, which involved direct injection of Borrelia burgdorferi spirochetes into the knee joints of C56Bl/6 mice; this model was then applied in mice lacking expression of certain pattern recognition receptors and components of the inflammasome (also on a C56Bl/6 background) to enable key molecules involved in the immune response to B. burgdorferi to be identified.
The central role of IL-1 in the pathogenesis of Lyme disease in mice—as shown previously in human disease—was confirmed: IL-1 receptor knock-out mice were protected against development of Lyme disease, showing considerably reduced joint swelling and reduced influx of inflammatory cells compared with wild-type mice (see figure).
Next, Oosting et al. studied the response to B. burgdorferi in vitro (cytokine production and inflammasome activation by bone marrow-derived macrophages) and in vivo in a range of genetically modified mice. In their model, development of Lyme arthritis was shown to require signalling via the TLR2–MYD88 pathway, whereas the NOD1–NOD2–RICK pathways have a minimal role.
Finally the authors report that B. burgdorferi-induced production of IL-1, and the development of Lyme disease, require activation of the inflammasome components ASC and caspase-1.
ORIGINAL RESEARCH PAPER
Oosting, M. et al. Murine Borrelia arthritis is highly dependent on ASC and caspase-1, but independent of NLRP3. Arthritis Res. Ther. 14, R247 (2012)
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Buckland, J. Insight into the innate immune response in Lyme disease. Nat Rev Rheumatol 9, 3 (2013). https://doi.org/10.1038/nrrheum.2012.210
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DOI: https://doi.org/10.1038/nrrheum.2012.210