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| Open AccessSingle cell analyses identify a highly regenerative and homogenous human CD34+ hematopoietic stem cell population
The trajectories of hematopoietic stem cells (HSCs) during lineage commitment are complex. Here, the authors use single cell RNA-sequencing and xenotransplantation to uncover CD34 + EPCR + (CD38/CD45RA)- HSCs, which high repopulating and self-renewal properties.
- Fernando Anjos-Afonso
- , Florian Buettner
- & Dominique Bonnet
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Article
| Open AccessEngineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics
Here, the authors use single cell RNA-sequencing to generate an atlas of signaling interactions regulating embryonic hematopoietic stem cell (HSC) development and apply this knowledge to engineer a niche sufficient to support HSC maturation in vitro.
- Brandon Hadland
- , Barbara Varnum-Finney
- & Irwin D. Bernstein
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| Open AccessA specialized bone marrow microenvironment for fetal haematopoiesis
The colonization of bone marrow by haematopoietic stem and progenitor cells is critical for lifelong blood cell formation. Here the authors report distinct features of fetal bone marrow and show that artery-derived signals promote haematopoietic colonization.
- Yang Liu
- , Qi Chen
- & Ralf H. Adams
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| Open AccessMulti-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment
During human embryonic development, haematopoietic stem cells of the foetal liver undergo expansion, while retaining engraftment capacity. Here the authors apply CITE-seq to profile single cells from a human foetal liver, identifying a molecular signature of engraftment potential
- Kim Vanuytsel
- , Carlos Villacorta-Martin
- & George J. Murphy
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Article
| Open AccessDynamic Runx1 chromatin boundaries affect gene expression in hematopoietic development
RUNX1 is a large and complex gene with two alternative promoters and multiple hematopoietic enhancers. Here the authors show that unlike smaller genes Runx1 becomes sub-compartmentalized during differentiation and gene activation. This subcompartmentalization partially depends on CTCF binding at promoter-proximal CTCF sites and transcription.
- Dominic D. G. Owens
- , Giorgio Anselmi
- & Marella F. T. R. de Bruijn
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| Open AccessEndothelial cell-specific expression of serine/threonine kinase 11 modulates dendritic cell differentiation
In the bone marrow, dendritic cell development is governed by supporting cells, such as endothelial cells. Here authors show that expression of serine/threonine kinase 11 in endothelial cells regulates differentiation of dendritic cell precursors via modulating secretion of stem cell factor.
- Qiang Zhao
- , Young-Min Han
- & Ming-Hui Zou
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| Open AccessPre-configuring chromatin architecture with histone modifications guides hematopoietic stem cell formation in mouse embryos
Here, the authors employed Hi-C and low-input itChIP-seq in four rare populations of the hematopoietic stem cell (HSC) ontogeny trajectory across early arterial endothelial cells (eAECs), hemogenic endothelial cells (HECs), pre-HSCs, and long-term HSCs (LT-HSCs) from mouse embryos to show that active histone modifications are largely set up in eAECs and 3D genome is then subsequently configured.
- Chen C. Li
- , Guangyu Zhang
- & Aibin He
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Article
| Open AccessEzh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors
Yolk sac erythro-myeloid progenitors (EMPs) are critical for embryo viability and a major source of adult tissue-resident macrophages. Here, the authors show an essential stage-specific role for Ezh2 in modulating Wnt signaling during EMP generation.
- Wen Hao Neo
- , Yiran Meng
- & Georges Lacaud
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Article
| Open AccessSingle-cell analysis identifies dynamic gene expression networks that govern B cell development and transformation
The characterisation of B cell progenitors could benefit from single cell RNA analysis. Here the authors show distinct transcriptional profiles of B cell progenitors which are dependent upon pre-BCR and these profiles can be related to B cell transformation in lymphoblastic leukaemia.
- Robin D. Lee
- , Sarah A. Munro
- & Michael A. Farrar
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| Open AccessDiscrete regulatory modules instruct hematopoietic lineage commitment and differentiation
Lineage differentiation and commitment is driven by transcription regulators and chromatin changes. Here the authors report daily profiling of chromatin accessibility and transcriptome changes during human erythropoiesis, relating these changes to lineage potential between erythropoiesis and megakaryopoieis.
- Grigorios Georgolopoulos
- , Nikoletta Psatha
- & Jeff Vierstra
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Article
| Open AccessIntrathymic differentiation of natural antibody-producing plasma cells in human neonates
The thymus is known as the organ of T lymphocyte development. Here authors show that terminal B cell differentiation also takes place in the thymus of human neonates, leading to antibody production against commensal and pathogenic bacteria.
- Hector Cordero
- , Rodney G. King
- & Emmanuel Zorn
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Article
| Open AccessSequential actions of EOMES and T-BET promote stepwise maturation of natural killer cells
Natural Killer cell development is controlled by two related transcription factors, Eomes and T-bet. Authors show here that while the two factors share a large proportion of their target genes, they regulate distinct developmental processes by differing in their pattern of expression and in their associated co-factors.
- Jiang Zhang
- , Stéphanie Le Gras
- & Thierry Walzer
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| Open AccessA unified model of human hemoglobin switching through single-cell genome editing
Genetic mechanisms underlying fetal hemoglobin (HbF) regulation and switching are not fully understood. Here, the authors develop a single-cell genome editing functional assay to model how effects of mutation-harbouring functional elements contribute to HbF expression.
- Yong Shen
- , Jeffrey M. Verboon
- & Vijay G. Sankaran
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Article
| Open AccessVertically transferred maternal immune cells promote neonatal immunity against early life infections
Maternal immune cells seed into the foetus during mammalian pregnancy, yet the functional role of these cells is unclear. Here the authors show that maternal immune cells in foetal bone marrow stimulate immune development, subsequently reducing the risk or severity of infections in newborns.
- Ina Annelies Stelzer
- , Christopher Urbschat
- & Petra Clara Arck
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Article
| Open AccessA connexin/ifi30 pathway bridges HSCs with their niche to dampen oxidative stress
Reactive oxygen species (ROS) are metabolic by-products which in excess can be toxic for hematopoietic stem and progenitor cells (HSPCs). Here the authors show that toxic ROS are transferred by expanding HSPCs to the zebrafish developmental niche via connexin Cx41.8, where Ifi30 promotes their detoxification.
- Pietro Cacialli
- , Christopher B. Mahony
- & Julien Y. Bertrand
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Article
| Open AccessReactivation of a developmentally silenced embryonic globin gene
Globin loci harbor genes that are expressed embryonically and silenced postnatally. Here the authors show that zeta-globin silencing depends upon selective hypoacetylation of its TAD subdomain, which blocks its interaction with the alpha-globin super-enhancer, and zeta-globin can be reactivated by acetylation.
- Andrew J. King
- , Duantida Songdej
- & Christian Babbs
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Article
| Open AccessMonocyte progenitors give rise to multinucleated giant cells
Multinucleated giant cells characterize granuloma formation in mycobacterial infections. Here the authors identify monocyte precursors with distinct immunological and metabolic properties as a source of the granuloma multinucleated giant cell compartment.
- Anne Kathrin Lösslein
- , Florens Lohrmann
- & Philipp Henneke
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| Open AccessIdentification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
Leukaemic stem cells drive acute myeloid leukaemia (AML) progression and relapse but they are incompletely characterized. Here, the authors combine single-cell transcriptomics and clonal tracking using nuclear and mitochondrial somatic variants to distinguish healthy, pre-leukaemic and leukaemic stem cells in AML.
- Lars Velten
- , Benjamin A. Story
- & Lars M. Steinmetz
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Article
| Open AccessThe genome-wide impact of trisomy 21 on DNA methylation and its implications for hematopoiesis
Down syndrome has a high co-morbidity with immune and hematopoietic disorders. Here, the authors perform an epigenome-wide association study in newborns with and without Down syndrome to find differential methylation across the genome, including in hematopoietic regulators RUNX1 and FLI1.
- Ivo S. Muskens
- , Shaobo Li
- & Adam J. de Smith
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| Open AccessEnvironmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice
Type 2 conventional dendritic cells (cDC2) are important immune activators in adults, but their development and functions at the neonatal stage remain unclear. Here the authors show, using fate-mapping and single-cell RNA sequencing, that neonatal cDC2 come from multiple origins, but converge functionally as potent immune activators upon proper stimuli.
- Nikos E. Papaioannou
- , Natallia Salei
- & Barbara U. Schraml
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Article
| Open AccessA clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy
T-cell immunotherapies, such as CAR-T immunotherapy, are being developed against a wide variety of diseases. Here the authors report the feeder-free, scalable differentiation of human induced pluripotent cells (iPSCs) to T-cells with T-cell receptor dependent anti-tumour function in vitro and in vivo.
- Shoichi Iriguchi
- , Yutaka Yasui
- & Shin Kaneko
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| Open AccessThe order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus
Developing T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocytes to show that, in selection intermediates, TCR signaling strength informs coreceptor expression timing.
- Mohammad M. Karimi
- , Ya Guo
- & Matthias Merkenschlager
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Article
| Open AccessThymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells
Innate-like T cells such as invariant natural killer T (iNKT) and mucosal-associated invariant T (MAIT) cells both develop in the thymus. Here the authors use single-cell RNA sequencing to show that mouse iNKT and MAIT share components of developmental regulation, with a transcription factor, Hivep3, implicated for the maturation of both cell types.
- S. Harsha Krovi
- , Jingjing Zhang
- & Laurent Gapin
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| Open AccessPlatelet-derived microparticles enhance megakaryocyte differentiation and platelet generation via miR-1915-3p
Platelets derive from megakaryocytes, which differentiate from hematopoietic stem/progenitor cells (HSPCs). Here, Qu et al show that platelet-derived microparticles carrying miR-1915-3p target HSPCs and promote megakaryopoiesis by suppressing RHOB expression levels.
- Mingyi Qu
- , Xiaojing Zou
- & Xuetao Pei
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Article
| Open AccessOntogeny of arterial macrophages defines their functions in homeostasis and inflammation
Arterial macrophages develop from either yolk sac or bone marrow progenitors. Here, the author show that ageing-induced reduction of arterial macrophages is not replenished by bone marrow-derived cells, but under inflammatory conditions circulating monocytes are recruited to maintain homeostasis, while arterial macrophages of yolk sac origin carry out tissue repair.
- Tobias Weinberger
- , Dena Esfandyari
- & Christian Schulz
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Article
| Open AccessSingle-cell transcriptome maps of myeloid blood cell lineages in Drosophila
How the Drosophila lymph gland hemocytes develop and are regulated at a single-cell level is unclear. Here, the authors use single-cell RNA sequencing to show heterogeneity of developing hemocytes in the lymph gland and how they react to wasp infestation, and compare hemocytes from two independent origins.
- Bumsik Cho
- , Sang-Ho Yoon
- & Jiwon Shim
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Article
| Open AccessGeneration, localization and functions of macrophages during the development of testis
The developmental origins and functions of testis macrophages remain incompletely characterized. Here, the authors show, using histology, high-dimensional mass cytometry and cell fate-mapping data, that interstitial and peritubular macrophages originate from distinct precursors and contribute distinctly to spermatogenesis.
- Emmi Lokka
- , Laura Lintukorpi
- & Marko Salmi
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Article
| Open AccessMicro-environmental sensing by bone marrow stroma identifies IL-6 and TGFβ1 as regulators of hematopoietic ageing
Ageing of the haematopoietic system is accompanied by declining erythropoiesis and lymphopoiesis. Here the authors uncover upregulated IL-6 and TGFβ signalling in aged bone marrow stroma; inhibition of these signals reverses age-related haematopoietic defects, re-balancing haematopoietic stem cell lineage output.
- Simona Valletta
- , Alexander Thomas
- & Claus Nerlov
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Article
| Open AccessSpinal cord injury causes chronic bone marrow failure
Spinal cord injury (SCI) often leads to immune dysfunction, but mechanistic insights are still lacking. Here the authors show that SCI alters chemokine signaling and induces long, persisting defects in hematopoietic stem and progenitor cell migration, thereby entrapping them in the bone marrow and disrupting peripheral immune homeostasis.
- Randall S. Carpenter
- , Jessica M. Marbourg
- & Phillip G. Popovich
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| Open AccessDisruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
The molecular basis of the clinically important MAM blood group antigen present in most humans is unknown. We identify EMP3 as its encoding gene, establishing MAM as a new blood group system, and demonstrate the role of EMP3 in erythropoiesis through its interaction with the signalling molecule CD44.
- Nicole Thornton
- , Vanja Karamatic Crew
- & David J. Anstee
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Article
| Open AccessAn Erg-driven transcriptional program controls B cell lymphopoiesis
B cell development is tightly regulated in a stepwise manner to ensure proper generation of repertoire diversity via somatic gene rearrangements. Here, the authors show that a transcription factor, Erg, functions at the earliest stage to critically control two downstream factors, Ebf1 and Pax5, for modulating this gene rearrangement process.
- Ashley P. Ng
- , Hannah D. Coughlan
- & Warren S. Alexander
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Article
| Open AccessDistinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins
Renal macrophages (RMs) can be of bone marrow or embryonic origin, but their abundance, fate and metabolic profiles in physiological and pathogenic settings are still unclear. Here the authors show, by characterizing these two RMs in multiple transgenic mouse lines, that they exhibit distinct dynamics, homeostasis, immune activity, and metabolic properties.
- Fengming Liu
- , Shen Dai
- & Xuebin Qin
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| Open AccessInterrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing
Tissues-specific gene expression requires coordinated cis-regulatory elements. Here the authors use dCas9-based enhancer targeting to remodel local epigenetic landscapes and activate or inactive transcription.
- Kailong Li
- , Yuxuan Liu
- & Jian Xu
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Article
| Open AccessMechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1
PU.1 is a master TF of hematopoietic lineage differentiation. Here the authors analyse properties of PU.1 DNA-binding in vitro and genome-wide in vivo across different cell types with native or ectopic PU.1 expression, and uncover the mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1.
- Julia Minderjahn
- , Andreas Schmidt
- & Michael Rehli
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| Open AccessGM-CSF drives dysregulated hematopoietic stem cell activity and pathogenic extramedullary myelopoiesis in experimental spondyloarthritis
Spondyloarthritis pathology is manifested by increased myeloid infiltration of the joints. Here the authors show that in a mouse model of spondyloarthritis, a single dose of a microbial ligand curdlan induces persistent extramedullary myelopoiesis in the spleen and joints, which is driven by GM-CSF and can be amplified by exogenous IL-33.
- Daniel Regan-Komito
- , James W. Swann
- & Thibault Griseri
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Article
| Open AccessTGF-β induces ST2 and programs ILC2 development
TGF-β is thought to be important for group 2 innate lymphoid cell (ILC2) function. Here the authors show that TGF-β drives expression of ST2 specifically in ILC2 progenitors and thereby is also important for the development of ILC2s in the bone marrow.
- Li Wang
- , Jun Tang
- & WanJun Chen
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Article
| Open AccessMyc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth
Thymic epithelial cells (TEC) are essential for the maturation of functional T cells, while thymus size is proportional to the overall output efficiency. Here the authors show, using transcriptome analyses, that mouse fetal TEC maintain a Myc-dependent genetic program to ensure a rapid increase in thymus size, and thereby expedited T cell generation.
- Jennifer E. Cowan
- , Justin Malin
- & Avinash Bhandoola
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Article
| Open AccessA revised model for promoter competition based on multi-way chromatin interactions at the α-globin locus
The coordination of interactions between multiple regulatory elements and genes within a chromatin domain remains poorly understood. Here, the authors use a method to detect multi-way chromatin interactions in a mouse model in which the α-globin domain is extended to include several additional genes, finding that the promoters do not form mutually exclusive interactions with the enhancers, but all interact simultaneously in a single complex.
- A. Marieke Oudelaar
- , Caroline L. Harrold
- & Jim R. Hughes
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Article
| Open AccessToll-like receptor 2 expression on c-kit+ cells tracks the emergence of embryonic definitive hematopoietic progenitors
There is limited knowledge of markers to identify various waves of murine embryonic hematopoiesis. Here, the authors show that the expression of toll-like receptor 2 (TLR2) on E7.5 c-kit+ cells marks the emergence of erythro-myeloid progenitor precursors and that the Tlr2 locus is active in E8.5 precursors of adult HSCs.
- Jana Balounová
- , Iva Šplíchalová
- & Dominik Filipp
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Article
| Open AccessThe Firre locus produces a trans-acting RNA molecule that functions in hematopoiesis
LncRNA loci potentially contain multiple modes that can exert function, including DNA regulatory elements. Here, the authors generated genetic models in mice to dissect the role of the syntenically conserved lncRNA Firre in the context of hematopoiesis.
- Jordan P. Lewandowski
- , James C. Lee
- & John L. Rinn
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Article
| Open AccessFunctional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells
Previous gene editing in haematopoietic stem cells (HSCs) has focussed on a heterogeneous CD34+ population. Here, the authors demonstrate high efficiency CRISPR/Cas9-based editing of purified long-term HSCs using non-homologous end joining and homology-directed repair, by directing isoform-specific expression of GATA1.
- Elvin Wagenblast
- , Maria Azkanaz
- & Eric R. Lechman
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Article
| Open AccessGenetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
Here, the authors show that reticulocytes derived from immortalized erythroblasts support invasion and development of Plasmodium falciparum and use CRISPR-mediated gene knockout and complementation of an invasion receptor to demonstrate utility of this model system for research in malaria invasion.
- Timothy J. Satchwell
- , Katherine E. Wright
- & Jake Baum
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Article
| Open AccessBlood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium
HSCs emerge from haemogenic endothelium (HE) in the dorsal aorta but whether these tissues share a common lineage is unclear. Here, the authors use a zebrafish runx1 reporter to show that HE maintains an arterial gene expression profile in the absence of Runx1, suggesting the aortic endothelium as a precursor of HE.
- Florian Bonkhofer
- , Rossella Rispoli
- & Roger Patient
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Article
| Open AccessThe histone methyltransferase Setd2 is indispensable for V(D)J recombination
The repertoire of adaptive immune receptor is generated by V(D)J recombination, somatic rearrangements of V, D and J gene segments, in the respective loci. Here the authors show that the deficiency of Setd2, a histone methyl transfer, impairs V(D)J recombination and induces severe developmental blocks in both T and B lineages.
- Zhongzhong Ji
- , Yaru Sheng
- & Helen He Zhu
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Article
| Open AccessDeletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations
The lineage-specific receptor CSF1R controls macrophage development and homeostasis. Here the authors show that deletion of a conserved Csf1r enhancer (FIRE) selectively depletes brain microglia and resident macrophages in the epidermis, kidney, heart and peritoneum of otherwise healthy mice.
- Rocío Rojo
- , Anna Raper
- & Clare Pridans
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Article
| Open AccessPrimary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish
Haematopoietic stem and progenitor cells (HSPCs) produce all blood lineages and arise from the haemogenic endothelium (HE) during embryogenesis. Here the authors show that genes specific to cilia formation are required for HSPC development in the HE in zebrafish through transduction of Notch signal.
- Zhibin Liu
- , Haiqing Tu
- & Feng Liu
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Article
| Open AccessThe role of platelets in mediating a response to human influenza infection
Influenza viremia is rare in human blood and not well studied. Here, the authors show that influenza can be found in human platelets and that platelet engulfment of influenza A results in TLR7-dependent C3 release, which in turn promotes neutrophil-DNA release and formation of platelet-DNA aggregates.
- Milka Koupenova
- , Heather A. Corkrey
- & Jane E. Freedman
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Article
| Open AccessGenetic programming of macrophages generates an in vitro model for the human erythroid island niche
In vitro differentiation of red blood cells (RBCs) is a desirable therapy for various disorders. Here the authors develop a culture system using stem cell-derived macrophages to show that inducible expression of a transcription factor, KLF1, enhances RBC production, potentially through the induction of three soluble factors, ANGPTL7, IL33 and SERPINB2.
- Martha Lopez-Yrigoyen
- , Cheng-Tao Yang
- & Lesley M. Forrester
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| Open AccessLong non-coding RNAs discriminate the stages and gene regulatory states of human humoral immune response
Long non-coding RNAs (lncRNA) constitute a large fraction of human transcriptome, and are reported, individually, for context-specific regulatory functions. Here the authors expand our understanding by providing a systemic, unbiased annotation of lncRNA to establish an atlas of lncRNA landscape during the induction of human humoral immune responses.
- Xabier Agirre
- , Cem Meydan
- & Ari Melnick