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| Open AccessFibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice
Beiging and thermogenesis in white adipose tissue (WAT) is an important adaptive response to cold exposure, but how the brain senses cold and subsequently induces beiging remains unclear. Here, the authors show that sympathetic nerves stimulate lymph nodes to release IL-33, thereby mediating cold-induced beiging of WAT.
- Lai Yee Cheong
- , Baile Wang
- & Aimin Xu
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Article
| Open AccessInfluence of circadian clocks on adaptive immunity and vaccination responses
Circadian rhythms have been shown to influence immune responses, but it is unclear whether this influences responses to vaccines. Here the authors show that dendritic cells migrate in a circadian rhythm meaning that interactions with T cells are altered leading to differential vaccine responses.
- Louise Madeleine Ince
- , Coline Barnoud
- & Christoph Scheiermann
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| Open AccessPostnatal expansion of mesenteric lymph node stromal cells towards reticular and CD34+ stromal cell subsets
Lymph nodes in various locations of the body differ in their cell composition and gene expression signatures. Here authors show that the rapid postnatal expansion of lymph nodes is governed by CD34 + stromal cells and fibroblastic reticular stromal cell progenitors, distinguished by intrinsic, microbiome-independent core epigenetic blueprints.
- Joern Pezoldt
- , Carolin Wiechers
- & Jochen Huehn
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Article
| Open AccessCD8 lymphocytes mitigate HIV-1 persistence in lymph node follicular helper T cells during hyperacute-treated infection
Despite antiretroviral therapy, HIV often persists in tissue sanctuary sites. In this work, authors investigate HIV persistence and T cell responses in lymph node biopsies obtained from individuals who initiated antiretroviral therapy in Fiebig stage I and beyond.
- Omolara O. Baiyegunhi
- , Jaclyn Mann
- & Zaza M. Ndhlovu
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| Open AccessRecycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift
Activated B cell enter germinal centers (GC) to become plasma cells and memory B cells. Here the authors show that some memory B cells recycle to GC via CCL-21 mediated chemotaxis to deliver antigens from the lymph node subcapsular sinus (SCS) to potentially contribute to affinity maturation and antigenic drift.
- Yang Zhang
- , Laura Garcia-Ibanez
- & Kai-Michael Toellner
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| Open AccessSIV-induced terminally differentiated adaptive NK cells in lymph nodes associated with enhanced MHC-E restricted activity
NK cells control SIV infection in secondary lymphoid tissues in the natural host that typically doesn’t progress toward disease. Here the authors show that this control is associated with terminal NK cell differentiation and improved MHC-E-dependent activity lacking in pathogenic SIV infection.
- Nicolas Huot
- , Philippe Rascle
- & Michaela Müller-Trutwin
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Article
| Open AccessCosmc controls B cell homing
Migration and homing of B cells to lymph nodes are important for B cell functions, but their regulation is poorly understood. Here, the authors show that B cell-specific deletion of Cosmc results in decreased protein O-glycosylation, loss of B cell homing to both lymphoid and nonlymphoid organs, and altered transendothelial migration implicated in this loss.
- Junwei Zeng
- , Mahmoud Eljalby
- & Richard D. Cummings
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| Open AccessA molecular map of murine lymph node blood vascular endothelium at single cell resolution
The origin and diversity of blood vascular endothelial cells (BEC) in lymphoid tissues is unclear. Here, the authors profile murine BECs from peripheral lymph nodes by single cell analysis and identify subsets of cells specialised for immune cell recruitment and vascular homeostasis.
- Kevin Brulois
- , Anusha Rajaraman
- & Eugene C. Butcher
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Article
| Open AccessB cell zone reticular cell microenvironments shape CXCL13 gradient formation
Morphogens such as chemokines form gradients to direct graded responses and modulate cell behaviors. Here the authors show, using imaging and computer simulation, that the chemokine CXCL13 originated from follicular reticular cells in the lymph nodes forms both soluble and immobilized gradients to regulate B cell recruitment and migration.
- Jason Cosgrove
- , Mario Novkovic
- & Mark C. Coles
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| Open AccessEfficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance
Immune cells mostly enter lymph nodes (LN) from blood circulation, but whether afferent lymphatics contributes to LN entry is unclear. Here, the authors show, using a photo-convertible reporter, that T cells in afferent lymphatics frequently enter LN and become arrested in the subcapsular sinus, with chemokines and integrins further guiding their migration in the LN.
- Rieke Martens
- , Marc Permanyer
- & Reinhold Förster
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| Open AccessLymphatic endothelial cells prime naïve CD8+ T cells into memory cells under steady-state conditions
Lymphatic endothelial cells (LECs) can cross-present antigen to naïve CD8+ T cells, but the significance of this interaction was unclear. Here the authors show that LECs directly induce CD8+ T cell differentiation with memory-like phenotypes, migration patterns and transcriptome, which can later be recalled to promote effector immunity and protection from Listeria infection.
- Efthymia Vokali
- , Shann S. Yu
- & Melody A. Swartz
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| Open AccessYAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells
Fibroblastic reticular cells (FRC) are important for lymph node (LN) structure and function. Here the authors show that the YAP/TAZ complex downstream of Hippo signalling regulates FRC commitment and maturation, with YAP/TAZ deficiency impairing FRC differentiation, while hyperactivation of YAZ/TAZ inducing myofibroblastic FRCs and LN fibrosis.
- Sung Yong Choi
- , Hosung Bae
- & Gou Young Koh
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| Open AccessNeonatally imprinted stromal cell subsets induce tolerogenic dendritic cells in mesenteric lymph nodes
Induction of tolerance in the gut relies on immunomodulatory functions of mesenteric lymph nodes (mLN). Here the authors show that mLN stromal cells receive early microbiota imprinting in the neonatal phase to exhibit long-term, location-specific transcriptional programs for the induction of regulatory T cells and peripheral tolerance.
- Joern Pezoldt
- , Maria Pasztoi
- & Jochen Huehn
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| Open AccessMemory B cells are reactivated in subcapsular proliferative foci of lymph nodes
Memory B cells need to be reactivated to produce high affinity antibody responses on subsequent antigen encounters. Here the authors show that memory B cells localise to lymph node subcapsular proliferative foci (SPF), which have distinct properties from the germinal centre, for rapid expansion and the induction of B memory responses.
- Imogen Moran
- , Akira Nguyen
- & Tri Giang Phan
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| Open AccessInduction of anergic or regulatory tumor-specific CD4+ T cells in the tumor-draining lymph node
Tumor neoantigens can be drained to the lymph nodes, but the nature and the significance of the induced immune responses are still unclear. Here the authors use a mouse genetic tumor model to show that tumor-specific CD4 T cells can become anergic or suppressive in the draining lymph node to modulate tumor immunity.
- Ruby Alonso
- , Héloïse Flament
- & Olivier Lantz
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| Open AccessMigratory dendritic cells acquire and present lymphatic endothelial cell-archived antigens during lymph node contraction
Viral infection and vaccination both induce lasting persistence of antigens for protective responses. Here the authors show that migratory dendritic cells, independent of the transcription factor BatF3 for their development, contribute to “archived antigen” exchange with lymphatic endothelial cells.
- Ross M. Kedl
- , Robin S. Lindsay
- & Beth A. Jirón Tamburini
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| Open AccessInteractions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis
The growth of lymph nodes in response to infection requires lymphangiogenesis. Dubey et al. show that the mesenteric lymph node lymphangiogenesis upon helminth infection depends on the signaling loop between the B and fibroblastic reticular cells (FRCs), whereby the FRCs respond to lymphotoxin secreted by B cells by releasing B cell activating factor.
- Lalit Kumar Dubey
- , Praneeth Karempudi
- & Nicola L. Harris
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Infection-induced type I interferons activate CD11b on B-1 cells for subsequent lymph node accumulation
Tissue-resident B-1 cells express CD11b, unlike their lymphoid organ-residing counterparts. Here the authors show that influenza-induced type I interferons activate CD11b on B-1 cells, facilitating entry to mediastinal lymph nodes, where they provide the first line of antibody-mediated host defense.
- Elizabeth E. Waffarn
- , Christine J. Hastey
- & Nicole Baumgarth
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| Open AccessCCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes
Innate lymphoid cells have an important role in mucosal immunity and present peptide:MHCII to CD4 T cells. Here the authors show that innate lymphoid cell subsets migrate from the gut mucosa to the draining lymph nodes via different mechanisms, where they form distinct microenvironments.
- Emma C. Mackley
- , Stephanie Houston
- & David R. Withers
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Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade
Thymic stromal lymphopoietin is an important initiator of Th2 responses, but, to date, little is known about how it drives the immune cascade in the tissue microenvironment in vivo. Here, the authors show that skin thymic stromal lymphopoietin orchestrate interactions between immune cells, leading to Th2 priming.
- Juan Manuel Leyva-Castillo
- , Pierre Hener
- & Mei Li