Nucleoskeleton articles within Nature Communications

Featured

• Article
  16 May 2024 | Open Access

  Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment

  This study combines lattice light sheet microscopy and single molecule imaging to study protein dynamics and chromatin structure in live cells. The authors describe how nucleosomes and proteins move and are organised in relation to chromatin density.

  • Timothy A. Daugird
  • , Yu Shi
  •  & Wesley R. Legant

• Article
  25 March 2021 | Open Access

  Apico-basal cell compression regulates Lamin A/C levels in epithelial tissues

  The nuclear lamina bridges mechanical forces from the cytoskeleton to the nucleus, and while Lamin A/C is known to be crucial for this process, its regulation remains unclear. Here the authors show that levels of Lamin A/C scale with apico-basal compression of cells independently of tissue stiffness using Drosophila epithelial tissues and mammalian cells.

  • K. Venkatesan Iyer
  • , Anna Taubenberger
  •  & Frank Jülicher

• Article
  04 February 2020 | Open Access

  DNA repair by Rad52 liquid droplets

  Genome dynamics allow cells to repair DNA double-strand breaks (DSBs), which are highly toxic DNA lesions. Here the authors reveal that in S. cerevisiae, Rad52 DNA repair proteins assemble in liquid droplets that work with dynamic nuclear microtubules to relocalize lesions to the nuclear periphery for repair.

  • Roxanne Oshidari
  • , Richard Huang
  •  & Karim Mekhail

• Article
  21 August 2019 | Open Access

  Structural basis for lamin assembly at the molecular level

  Lamins are intermediate filaments and the major component of the nuclear lamina. Here the authors determine the crystal structure of a construct comprising the N-terminal half of human lamin A/C and use their structure and cross-linking and biochemical experiments to discuss lamin assembly.

  • Jinsook Ahn
  • , Inseong Jo
  •  & Nam-Chul Ha

• Article
  11 July 2019 | Open Access

  Lamin A molecular compression and sliding as mechanisms behind nucleoskeleton elasticity

  Lamin A is critical for nuclear architecture but its structure and assembly are not fully understood. Here, the authors use quantitative cross-linking mass spectrometry to map intra- and intermolecular interactions within lamin homomers, providing insights into the molecular basis for lamin’s mechanical properties.

  • Alex A. Makarov
  • , Juan Zou
  •  & Eric C. Schirmer

• Article
  02 July 2018 | Open Access

  Nuclear microtubule filaments mediate non-linear directional motion of chromatin and promote DNA repair

  Following DNA damage, different processes come to action to aid repair. The authors here find that microtubule filaments within the cell nucleus capture and non-randomly mobilize damaged chromatin to mediate DNA repair.

  • Roxanne Oshidari
  • , Jonathan Strecker
  •  & Karim Mekhail

• Article
  14 December 2017 | Open Access

  Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology

  An actin cap protects the morphology of the nucleus during cellular mechanical stress. Here, the authors show that the nuclear lamina protein lamin A/C mediates the formation of the actin cap in response to stress, and model the distribution of forces in the presence and absence of the actin cap.

  • Jeong-Ki Kim
  • , Arghavan Louhghalam
  •  & Dong-Hwee Kim