RNA transport articles within Nature Communications

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  • Article
    | Open Access

    SNURPORTIN-1, encoded by the SNUPN gene, plays a key role in the nuclear import of spliceosomal small nuclear ribonucleoproteins, however its physiological function remains unclear. Here the authors report that recessive SNUPN mutations cause a distinct subtype of childhood muscular dystrophy and reveal SNURPORTIN-1’s role in muscle homeostasis, offering insights for new therapeutic strategies.

    • Marwan Nashabat
    • , Nasrinsadat Nabavizadeh
    •  & Nathalie Escande-Beillard

  • Article
    | Open Access

    UAP56 is an important factor in the TREX complex, which is responsible for mRNA export. Here the authors show that the closely related RNA helicases, UAP56 and URH49, exhibit different three-dimensional structures due to one amino acid change. Accordingly, they form distinct apo-complexes and function in the nuclear export of specific target mRNAs.

    • Ken-ichi Fujita
    • , Misa Ito
    •  & Seiji Masuda

  • Article
    | Open Access

    Here, Bhat et al. show that Influenza A virus mRNAs are exported from the nucleus via the nucleoporin Tpr and the mRNA export complex TREX-2. These mRNAs have low exon number, high mean exon length, and low GC content. A 45-nucleotide RNA signal can mediate export via TREX-2.

    • Prasanna Bhat
    • , Vasilisa Aksenova
    •  & Beatriz M. A. Fontoura

  • Article
    | Open Access

    The UCLA Ribonomics group reports that the nuclear export efficiency of innate immune mRNAs varies over a hundred-fold range such that for many genes only a small fraction of the newly synthesized premRNA reaches the cytoplasm. They show that nuclear export and cytoplasmic decay rates are correlated thereby ensuring similar expression levels of short-lived and long-lived mRNAs.

    • Diane Lefaudeux
    • , Supriya Sen
    •  & Sri Kosuri

  • Article
    | Open Access

    There is little information on export directionality of RNA passing through the nuclear pore complex in human cells. Here, the authors examine single RNA molecules in transit, to demonstrate 5’-first directionality for the export of mRNA and lncRNA.

    • Asaf Ashkenazy-Titelman
    • , Mohammad Khaled Atrash
    •  & Yaron Shav-Tal

  • Article
    | Open Access

    This study identifies the evolutionarily conserved Exportin 4 as an essential regulator in the nuclear export of circRNAs. Defective circRNA export results in R-loop formation and DNA damage in cells, as well as testis and neurological defects in mice.

    • Liang Chen
    • , Yucong Wang
    •  & Ge Shan

  • Article
    | Open Access

    Aberrant RNA condensates are a hallmark of age-related neurodegenerative diseases. Here, the authors show that RNA condensation increases in aging Drosophila brains, triggering translation repression.

    • Kavya Vinayan Pushpalatha
    • , Mathilde Solyga
    •  & Florence Besse

  • Article
    | Open Access

    RNA granules are important regulators of RNA metabolism. Here the authors report that RNA granules containing RNA helicase DDX6 disassemble during neuronal maturation.

    • Karl E. Bauer
    • , Niklas Bargenda
    •  & Michael A. Kiebler

  • Article
    | Open Access

    Gene-gating of a MYC oncogenic super-enhancer (OSE) increases its expression in colon cancer cells in a poorly understood process. Here the authors show that MYC gating requires a CTCF binding site (CTCFBS) within the OSE that directs the stepwise trafficking of the OSE to the nuclear pore to facilitate increased nuclear export of MYC mRNA, which results in a growth advantage.

    • Ilyas Chachoua
    • , Ilias Tzelepis
    •  & Anita Göndör

  • Article
    | Open Access

    It is increasingly recognised that the spatial localisation of RNA is important for proper cellular function. Here, the authors investigate RNA localisation in skeletal muscle and develop methods to show that global active transport of RNA is required to maintain dispersion of gene products in the large muscle syncytium.

    • Lance T. Denes
    • , Chase P. Kelley
    •  & Eric T. Wang

  • Article
    | Open Access

    New contractile units are required during cardiac hypertrophy, though it remains unclear precisely where and how these new sarcomeres are added. Here the authors reveal that in the heart, microtubules spatiotemporally regulate mRNAs and ribosomes to build new sarcomeres, a role which is essential for growth.

    • Emily A. Scarborough
    • , Keita Uchida
    •  & Benjamin L. Prosser

  • Article
    | Open Access

    mRNAs export from the nucleus is thought to be regulated in part by three nucleoporins that comprise the nuclear basket, but whether and how distinct basket nucleoporins interact with the RNA export machinery is unclear. Here, the authors use rapid auxin-mediated degradation of basket nucleoporins Nup153, Nup50, and Tpr, and see that Tpr interacts with the TREX-2 mRNA export complex.

    • Vasilisa Aksenova
    • , Alexandra Smith
    •  & Mary Dasso

  • Article
    | Open Access

    Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications. Here the authors present an array of stably folding Mango II aptamers for imaging of coding and non-coding RNAs at single-molecule resolution, in both live and fixed cells.

    • Adam D. Cawte
    • , Peter J. Unrau
    •  & David S. Rueda

  • Article
    | Open Access

    Asymmetric subcellular mRNA distribution is important for local translation of neuronal mRNAs. Here the authors employed MS2 live-cell imaging and showed that the reporter mRNA containing the 3’ UTR of Rgs4 shows an anterograde transport bias, dependent on neuronal activity and the protein Staufen2, and mediates sustained mRNA recruitment to synapses.

    • Karl E. Bauer
    • , Inmaculada Segura
    •  & Michael A. Kiebler

  • Article
    | Open Access

    Large protein complexes and ribonucleoprotein particles (RNPs) such as pre-ribosomes are transported from the nucleus to the cytoplasm through the nuclear pore complex (NPC). Here the authors use ultrafast freezing and electron tomography to catch snapshots of native RNPs crossing the NPC and estimate their transit time using a probabilistic model.

    • Franck Delavoie
    • , Vanessa Soldan
    •  & Pierre-Emmanuel Gleizes

  • Article
    | Open Access

    The export of mRNA to the cytosol depends on the nuclear pore complex (NPC) and the activation of the helicase DDX19, but their interplay in humans remains poorly understood. Here, the authors present a structural and functional analysis of DDX19 activation, revealing how the human NPC regulates mRNA export.

    • Daniel H. Lin
    • , Ana R. Correia
    •  & André Hoelz

  • Article
    | Open Access

    Adenomatous polyposis coli (APC) regulates the localization of some mRNAs at cellular protrusions but the underlying mechanisms and functional roles are not known. Here the authors show that APC-dependent RNAs are enriched in contractile protrusions, via detyrosinated microtubules, and enhance cell migration.

    • Tianhong Wang
    • , Susan Hamilla
    •  & Stavroula Mili

  • Article
    | Open Access

    Subcellular localization of RNAs and proteins is important for polarized cells such as neurons. Here the authors differentiate mouse embryonic stem cells into neurons, and analyze the local transcriptome, proteome, and translated transcriptome in their cell bodies and neurites, providing a unique resource for future studies on neuronal polarity.

    • Alessandra Zappulo
    • , David van den Bruck
    •  & Marina Chekulaeva

  • Article
    | Open Access

    The processing of RNAs transcribed by RNA polymerase II requires a cap-binding complex (CBC), consisting of NCBP1 and NCBP2. Here, the authors report an alternative CBC formed by NCBP1 and a previously uncharacterized protein, NCBP3 that is critical for RNA processing under cellular stress conditions.

    • Anna Gebhardt
    • , Matthias Habjan
    •  & Andreas Pichlmair

  • Article
    | Open Access

    Nuclear import of transcriptional regulators and export of mRNA are essential steps for the manufacture of proteins in the cytoplasm. Here, Rajakylä et al.link these two activities by showing that the mRNA export factor Ddx19 promotes the nuclear import of the transcriptional coactivator MKL1.

    • Eeva Kaisa Rajakylä
    • , Tiina Viita
    •  & Maria K. Vartiainen

  • Article |

    Messenger RNAs and their associated proteins are transported from the nucleus through highly selective nuclear pore complexes. Using ultrahigh resolution single-molecule imaging, the authors visualise the path taken by each messenger RNA as it negotiates the pore’s selectivity filter.

    • Jiong Ma
    • , Zhen Liu
    •  & Weidong Yang

  • Article |

    The TREX complex and Nxf1 are involved in the export of mRNA from the nucleus but the precise molecular function of TREX is unclear. Here, the TREX components Aly and Thoc5 are shown to bind to Nxf1 resulting in a change in Nxf1 conformation that permits binding to mRNA and nuclear export.

    • Nicolas Viphakone
    • , Guillaume M. Hautbergue
    •  & Stuart A. Wilson

  • Article |

    Zonal occludens and claudin form tight junctions near the apical surface of cells and are important in polarized epithelia. In this study, the translational regulatory sequence-specific RNA binding protein CPEB is shown to be required for the correct localization of zona occluden 1 mRNA in mammary epithelial cells.

    • Kentaro Nagaoka
    • , Tsuyoshi Udagawa
    •  & Joel D. Richter

  • Article |

    The pre-mRNA splicing and TREX mRNA export machineries are found in nuclear speckle domains. Diaset al. microinject CMV-DNA constructs into cells and find that transcripts containing functional splice sites accumulate in nuclear speckles and that the TREX complex is required to release the mRNA once processed.

    • Anusha P. Dias
    • , Kobina Dufu
    •  & Robin Reed

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