Stem-cell biotechnology articles within Nature Communications

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  • Article
    | Open Access

    CRISPRa is an attractive tool for cellular reprogramming due to its multiplexing capacity and direct targeting of genomic loci. Here the authors demonstrate the reprogramming of human fibroblasts into iPSCs, which is enhanced by targeting a conserved Alu-motif.

    • Jere Weltner
    • , Diego Balboa
    •  & Timo Otonkoski

  • Article
    | Open Access

    Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.

    • Shin-Il Kim
    • , Tomoko Matsumoto
    •  & Knut Woltjen

  • Article
    | Open Access

    Induced pluripotent stem cells (iPSCs) have potential for regenerative medicine applications, but are generated with very low efficiency. Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA mimics, and optimized culture methods.

    • Igor Kogut
    • , Sandra M. McCarthy
    •  & Ganna Bilousova

  • Article
    | Open Access

    Torsade de Pointes (TdP) is a life-threatening ventricular arrhythmia often caused by drugs. In response to an urgent need for human tissue TdP models, here the authors describe a 3D human iPS cell-engineered heart tissue that generates TdP in response to drugs, providing a suitable model for studies of TdP mechanism and drug toxicity.

    • Masahide Kawatou
    • , Hidetoshi Masumoto
    •  & Jun K. Yamashita

  • Article
    | Open Access

    The derivation of blood progenitor cells from human pluripotent stem cells is of interest for cell therapy but remains an inefficient process. Here the authors micropattern hPSC-derived haemogenic endothelial (HE) cells into spatially-organized, size-controlled colonies and identify a geometry that achieves increased efficiency in deriving blood cells.

    • Nafees Rahman
    • , Patrick M. Brauer
    •  & Peter W. Zandstra

  • Article
    | Open Access

    The generation of a sustainable supply of erythroid progenitors is essential for the reliable production of anin vitroderived red blood cell clinical product. Here the authors immortalize early human erythroblasts to generate the first cell line capable of differentiation into functional adult reticulocytes.

    • Kongtana Trakarnsanga
    • , Rebecca E. Griffiths
    •  & Jan Frayne

  • Article
    | Open Access

    Platelets are blood circulating corpuscles generated from megakaryocytes that initiate wound healing. Here, Moreau et al. describe a way of producing large quantities of megakaryocytes from human pluripotent stem cells in the laboratory, moving us a step closer to manufacturing transfusion products.

    • Thomas Moreau
    • , Amanda L. Evans
    •  & Cedric Ghevaert

  • Article |

    When cultured as single cells, embryonic stem cells have low viability. Here, blebbistatin, a non-muscle myosin II inhibitor, is shown to enhance the cloning efficiency, viability and adhesion of both human embryonic stem cells and human induced pluripotent stem cellsin vitro.

    • Andrea Walker
    • , Hua Su
    •  & Noboru Sato

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