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A TAL effector repeat architecture for frameshift binding
Transcription activator-like effectors (TALEs) of pathogenic bacteria activate target genes in host plants to support infection. Here, the authors show that TALEs with single natural repeats of aberrant length tolerate one base pair deletions and may enable the bacteria to overcome natural plant resistance.
- Annekatrin Richter
- , Jana Streubel
- & Jens Boch
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Efficient genome engineering by targeted homologous recombination in mouse embryos using transcription activator-like effector nucleases
Genetically engineered mice are an important aspect of human disease research. Here, the authors use artificial transcription activator-like effector-nucleases to generate a mouse line with a conditionally targeted allele and suggest that this method can be easily adapted to any gene in the mouse genome.
- Daniel Sommer
- , Annika E. Peters
- & Marc Beyer
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Article
| Open Access
Compact designer TALENs for efficient genome engineering
Transcription activator-like effector nucleases (TALENs) are dimeric 'molecular scissors' that can be readily engineered for gene-targeting applications. Beurdeley et al. develop a single-chain TALEN architecture having significant in vivoactivity in yeast, plant and mammalian systems.
- Marine Beurdeley
- , Fabian Bietz
- & George H. Silva
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Article
| Open Access
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases
Hemimetabolous insects comprise many pests but introducing targeted mutations into these species has been difficult. This paper reports efficient targeted mutagenesis, and the generation of homozygous knockouts, in crickets based on zinc finger nucleases or transcription activator-like effector nucleases.
- Takahito Watanabe
- , Hiroshi Ochiai
- & Taro Mito
Developing mitochondrial base editors with diverse context compatibility and high fidelity via saturated spacer library