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| Open AccessSpatial control of avidity regulates initiation and progression of selective autophagy
The molecular principles governing the initiation of autophagosome formation are not clearly understood. Here we show that the vacuolar protein Vac8 coordinates this process by promoting an avidity-driven assembly of several autophagy factors.
- David M. Hollenstein
- , Mariya Licheva
- & Claudine Kraft
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Article
| Open AccessDstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway
Congenital scoliosis is a complex genetic disorder characterized by vertebral malformation. Here, the authors demonstrate that loss of dstyk leads to scoliosis in zebrafish due to dysregulated biogenesis of notochord vacuoles and that DSTYK is required for lysosome biogenesis through mTORC1 regulation.
- Xianding Sun
- , Yang Zhou
- & Lin Chen
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Article
| Open AccessNanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi
Vacuolar sorting receptors (VSRs) are suggested to efficiently transport hydrolases by continuous cycling. Here, the authors use a nanobody-epitope interaction-based labeling approach to trace VSR recycling from the TGN/EE to the cis-Golgi and reveal ligand reloading of recycled VSRs.
- Simone Früholz
- , Florian Fäßler
- & Peter Pimpl
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Article
| Open AccessA systematic exploration of the interactions between bacterial effector proteins and host cell membranes
Microbial pathogens secrete effector proteins into host cells to affect cellular functions. Here, the authors use a yeast-based screen to study around 200 effectors from six bacterial species, showing that over 30% of them interact with the eukaryotic plasma membrane or intracellular organelles.
- Bethany A. Weigele
- , Robert C. Orchard
- & Neal M. Alto
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Article
| Open AccessAtg4 proteolytic activity can be inhibited by Atg1 phosphorylation
The protease Atg4 mediates Atg8 lipidation, required for autophagosome biogenesis, but also triggers Atg8 release from the membranes, however is unclear how these steps are coordinated. Here the authors show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites.
- Jana Sánchez-Wandelmer
- , Franziska Kriegenburg
- & Fulvio Reggiori
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Article
| Open AccessA vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga
Coccolithophores are unicellular marine algae that produce calcitic particles inside their cells. Here the authors study cells of the dominant coccolithophore Emiliania huxleyiand identify an intracellular compartment that is filled with high concentrations of a disordered form of calcium.
- Sanja Sviben
- , Assaf Gal
- & André Scheffel