Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth

Dzamukova, Maria; Brunner, Tobias M.; Miotla-Zarebska, Jadwiga; Heinrich, Frederik; Brylka, Laura; Mashreghi, Mir-Farzin; Kusumbe, Anjali; Kühn, Ralf; Schinke, Thorsten; Vincent, Tonia L.; Löhning, Max

doi:10.1038/s41467-022-30618-8

Download PDF

Article
Open access
Published: 01 June 2022

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth

Nature Communications volume 13, Article number: 3059 (2022) Cite this article

7745 Accesses
20 Citations
10 Altmetric
Metrics details

Subjects

Abstract

Bone growth requires a specialised, highly angiogenic blood vessel subtype, so-called type H vessels, which pave the way for osteoblasts surrounding these vessels. At the end of adolescence, type H vessels differentiate into quiescent type L endothelium lacking the capacity to promote bone growth. Until now, the signals that switch off type H vessel identity and thus limit adolescent bone growth have remained ill defined. Here we show that mechanical forces, associated with increased body weight at the end of adolescence, trigger the mechanoreceptor PIEZO1 and thereby mediate enhanced production of the kinase FAM20C in osteoblasts. FAM20C, the major kinase of the secreted phosphoproteome, phosphorylates dentin matrix protein 1, previously identified as a key factor in bone mineralization. Thereupon, dentin matrix protein 1 is secreted from osteoblasts in a burst-like manner. Extracellular dentin matrix protein 1 inhibits vascular endothelial growth factor signalling by preventing phosphorylation of vascular endothelial growth factor receptor 2. Hence, secreted dentin matrix protein 1 transforms type H vessels into type L to limit bone growth activity and enhance bone mineralization. The discovered mechanism may suggest new options for the treatment of diseases characterised by aberrant activity of bone and vessels such as osteoarthritis, osteoporosis and osteosarcoma.

Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis

Article Open access 23 March 2021

Skeleton-vasculature chain reaction: a novel insight into the mystery of homeostasis

Article Open access 22 March 2021

Endothelial SMAD1/5 signaling couples angiogenesis to osteogenesis in juvenile bone

Article Open access 13 March 2024

Introduction

Proper skeleton development requires a fine-tuned crosstalk between different types of cells and tissues present in bone¹. Juvenile bones harbour highly angiogenic type H vessels, which are closely associated with and coordinate the differentiation of bone growth-mediating osteoblasts. In contrast, quiescent type L vessels are present in the adult bone^2,3,4. Type H vessels are located exclusively at the sites of active bone growth, namely the ossification front (OF) and periosteum, and they are organised in columnar structure². Notch signalling, Hypoxia-inducible factor 1-alpha (HIF1α) activity, blood flow speed and slit guidance ligand 3 (SLIT3) were shown to support type H vessel formation^3,5,6. However, the mechanism that directs the switch from type H to type L vessels resulting in bone growth cessation has remained unknown. A recent study reported that endothelial cells in bone require integrins for their proper functioning and maintenance, which highlights extracellular matrix (ECM) proteins as important factors for endothelium activity⁷. Yet, little is known about further ECM proteins controlling endothelial cells in bone. Therefore, we analysed changes in the composition of ECM proteins throughout bone development.

In this work we describe the molecular mechanism that links mechanical forces and angiogenesis in bone via mechanically triggered accumulation of the extracellular matrix protein dentin matrix protein 1 (DMP1) and its regulation of vascular subtypes.

Results

DMP1 is a potential candidate controlling endothelial cell fate in bone

To dissect age-related changes in vascular and perivascular microenvironments during and after active bone growth, we performed laser microdissection (LMD) of single capillary with associated surrounding cells in the OF of juvenile (4-week-old) and adult (12-week-old) mice. We developed a protocol to cut single capillary from undecalcified and unfixed bones of endothelial cell-specific GFP-reporter mice with a high RNA preservation rate to subsequently perform next-generation sequencing (NGS) (Supplementary Fig. 1a, b). Principal component analysis showed that the cells from the juvenile OF were very similar between different mice, whereas with ageing, greater heterogeneity was apparent (Supplementary Fig. 1c). To identify candidates from the list of differentially expressed genes with a potential of regulating type H vasculature, we focused on ECM proteins (Fig. 1a, b and Supplementary Fig. 1d). DMP1 appeared to be a promising candidate because of its reported angiogenesis-modulating activity in a retina tumour model⁸.

**Fig. 1: Burst in DMP1 secretion from osteoblasts into ECM at the OF-GP border during periadolescence.**

DMP1 is known as an essential player in matrix mineralisation^9,10. Our detailed analysis of DMP1 protein localisation throughout postnatal bone development showed that until 5 weeks of age, the protein was mainly localised at the base of the zone of type H vessels in the OF (Fig. 1c, d). Unexpectedly, at the age of 5.5–6 weeks, a burst in DMP1 secretion into ECM occurred primarily in the central top part of the OF also reaching the growth plate (GP) (Fig. 1c, e–g and Supplementary Fig. 2a). We performed quantifications of protein staining intensities within central and peripheral regions of interest in the OF (Fig. 1f, g and Supplementary Fig. 2b). During late postnatal bone maturation with increasing age, the DMP1-occupied area spread from the centre towards the periphery of the OF. Finally, by the age of 12 weeks, the whole metaphysis together with the GP were interspersed with DMP1 (Fig. 1c, f, g). This behaviour was seen irrespective of sex.

To identify the main cellular source of DMP1 in the OF-GP area at different ages, we established a method for RNAScope in bone. Co-hybridisation was performed with probes for Dmp1 together with Runx2 for early osteoblasts, osterix (Osx or Sp7) for mature osteoblasts and sclerostin (Sost) for osteocytes (Supplementary Fig. 2c). Sclerostin expression was not found in the OF but only in the cortical bone. At all analysed stages in the OF-GP region, Dmp1 mRNA was only detected in the OF area but never in the chondrocytes of the GP, including time points directly before (5 weeks) and during the burst in DMP1 protein secretion (5.5 weeks) (Fig. 1h and Supplementary Fig. 2f). Dmp1 mRNA was mainly found in Osx⁺ cells and to a lesser extent in Runx2⁺ cells at all examined time points, which identifies osteoblasts as the main producer of DMP1 in the metaphysis (Supplementary Fig. 2d, e). The number and intensity of Dmp1-expressing cells peaked at 4–5 weeks (Fig. 1i, j and Supplementary Fig. 2g). Yet, there was no difference at the level of Dmp1 gene expression between the central and peripheral parts of the OF, indicating that the observed differences in DMP1 amounts between these regions emerged rather at the protein level (cf. Fig. 1c, f, g). This observation goes in line with previous reports that the control of DMP1 availability and function mainly takes place at the posttranslational rather than transcriptional level^11,12,13 explaining the discrepancy in the timing of mRNA and protein peaks (Fig. 1c, f, g, i, j). Together, these data demonstrate that the burst in DMP1 secretion from osteoblasts in the OF centre at the age of 5.5–6 weeks is controlled at the protein level and DMP1 likely diffuses from the OF to the GP (Fig. 1k).

DMP1 transforms type H vessels into type L

In addition to secreting osteogenic factors³, tip cells of type H vessels secrete matrix metalloproteinase 9 (MMP9) that digests cartilage matrix and allows vessel bulges to invade GP cartilage, thereby facilitating bone growth⁴. We noticed that DMP1 accumulation in the OF centre at 5.5–6 weeks correlated with a decrease in endomucin (EMCN) intensity and reduced amounts of MMP9 at the OF-GP border. This was associated with the disappearance of bulges and columnar structure of the vessels (Fig. 2a, b and Supplementary Fig. 3). Hence, all main features of type H vessels were downregulated. Importantly, at 5.5–6 weeks, these changes occurred only in the central part of the OF.

**Fig. 2: DMP1 transforms active type H vessels into quiescent type L in the OF and in subchondral bone.**

To study whether DMP1 directly regulates bone angiogenesis and growth, we generated mice with a constitutive and an inducible deletion of Dmp1 (Supplementary Fig. 4). Constitutive Dmp1-deficient mice were smaller with shortened and widened long bones (Supplementary Fig. 4c–f) as had been described before^9,14, and they featured a disorganized OF and GP (Supplementary Fig. 5a). The blood vessels in the OF were dilated and had very high EMCN and MMP9 intensity, even in adulthood, though the columnar structure was lost already in juvenile bones (Supplementary Fig. 5c, d). This finding suggests that, presumably via its matrix mineralisation activity, DMP1 provides a scaffold for columns of type H vessels. The phenotype of Dmp1^+/− mice was identical to Dmp1^+/+ mice, which indicates that one Dmp1 allele generates sufficient DMP1 protein (Supplementary Figs. 4c, d and 5b). This result is consistent with human genetic studies where it was shown that mutations in the DMP1 gene cause autosomal recessive hypophosphatemia¹⁵. OSX⁺ and RUNX2⁺ osteoblasts were present in the OF to a similar extent as in the wild-type group indicating that DMP1 is not required for osteoblast differentiation and maturation¹⁶ (Supplementary Fig. 5a, b). Notably, the GP of Dmp1^-/- mice exhibited an expanded pre-hypertrophic and hypertrophic zone (RUNX2⁺ and SOX9⁺) (Supplementary Fig. 5a). We assume that this phenotype is caused by the lack of proper vessel invasion due to the loss of vessel architecture.

To test whether DMP1 induces the switch from type H to type L vessels, we generated a Dmp1-floxed mouse line and crossed it with Col1-CreERT2 mice to ablate Dmp1 in an inducible manner selectively in osteoblasts (Dmp1^i∆OB) upon tamoxifen injection (Supplementary Fig. 4g–i). Deletion of Dmp1 was performed at P28-32 to allow normal formation of the OF together with the columnar structure of blood vessels (Fig. 2c). Tamoxifen treatment led to ~75% reduction of Dmp1 mRNA expression by week 6 (P42) (Supplementary Fig. 6a, b) and reduced the amount of DMP1 protein in the OF centre by ~70% (Fig. 2d and Supplementary Fig. 6c). The body weight was not affected (Supplementary Fig. 6d). Yet, long bones of Dmp1^i∆OB mice featured an enlarged metaphysis with overall increased EMCN and MMP9 intensities (Fig. 2e, f and Supplementary Fig. 6e). In line with the femur phenotype of constitutive Dmp1-deficient mice (cf. Supplementary Fig. 4d, f), also Dmp1^i∆OB femurs were slightly shorter than control femurs by the age of 6 weeks, presumably reflecting the key role of DMP1 in matrix mineralisation and bone architecture (Supplementary Fig. 6f). Nevertheless, detailed analysis showed that the deletion of Dmp1 in osteoblasts arrested the age-related maturation of the OF. Specifically, type H vessels together with bulges were preserved in the central part of the OF in Dmp1^i∆OB mice (Fig. 2g, h). Moreover, the difference in EMCN and MMP9 intensity between the central and peripheral parts of the OF was absent in Dmp1^i∆OB femurs (Fig. 2i, k). The overall EMCN intensity and MMP9 amount in the OF centre were higher in Dmp1^i∆OB samples compared with the control group (Fig. 2j, l). As expected, von Kossa staining showed reduced mineralisation of the OF in Dmp1^i∆OB femurs, confirming the crucial importance of DMP1 for bone calcification (Supplementary Fig. 6g, h, j).

In line with the phenotype of Dmp1^i∆OB mice at the OF, analysis of subchondral bone revealed that Dmp1 deletion at 4 weeks led to preserved density of EMCN⁺ vessels and high amounts of MMP9 around the vessel tips underneath the articular cartilage by week 6 (Fig. 2m-o). Control mice featured significantly reduced vessel activity in subchondral bone, whereas Dmp1^i∆OB samples were very similar to 4-week-old wild-type subchondral bone. Thus, deletion of Dmp1 at 4 weeks prevented the development-associated switch from active to quiescent vessel fate not only at the OF but also in subchondral bone. The mineralisation of subchondral bone was also reduced in Dmp1^i∆OB femurs (Supplementary Fig. 6g, i, k). Hence, we cannot formally exclude that the reduced mineralisation contributes to some extent to the observed phenotype in Dmp1^i∆OB bones. Nevertheless, our results suggest that DMP1 is involved in the inhibition of angiogenesis in long bones and in the transformation of active type H into quiescent type L vessels, which results in reduced EMCN and MMP9 expression and loss of vessel bulges.

DMP1 inhibits VEGF signalling in the ossification front in vivo and in endothelial cells in vitro

Vascular endothelial growth factor (VEGF) signalling is crucial for angiogenesis and bone growth^17,18,19. The proliferation of the chondrocytes in the proliferative layer of the GP allows the bone to elongate, whereas the hypertrophic chondrocytes in the bottom layer express high amounts of VEGFA, which drives the invasion of type H vessels into the GP, thus leading to the progression of the ossification front^17,18. To test whether DMP1 has VEGF-inhibiting properties in bone, we studied phosphorylation of the main VEGF receptor VEGFR2 (pVEGFR2) along with DMP1 accumulation in ECM. Local DMP1 amounts negatively correlated with the amount of pVEGFR2 (Fig. 3a and Supplementary Fig. 7). The VEGFR2 phosphorylation level in 4- to 5-week-old mice was equal across the OF. However, at 5.5-6 weeks we observed a marked reduction in the amount of pVEGFR2 in the central part of the OF in wild-type mice. This difference was largely lost in Dmp1^i∆OB mice, and the overall intensity of pVEGFR2 in the OF centre was higher (Fig. 3b–d). Of note, the total level of VEGFR2 protein was not affected by Dmp1 deletion (Fig. 3e, g).

**Fig. 3: Extracellular DMP1 inhibits VEGF signalling in the OF in vivo and in endothelial cells in vitro.**

Previous studies showed that VEGFR2 activation induces Flt4 (VEGFR3) expression in endothelial cells^20,21. VEGFR3 is usually highly upregulated in endothelial tip cells, where it is required for vessel sprouting^7,20,22. Bulges in the GP of Dmp1^i∆OB mice stained strongly with VEGFR3 compared with remaining bulges of control mice (Fig. 3f, h). This result suggests that osteoblast-released DMP1 downregulates VEGFR3 in endothelial tip cells most likely by interference with VEGFR2 signalling, thus leading to the loss of vessel bulges. Further support came from the finding that treatment of the capillary endothelial cell line bEnd.3 with DMP1 abrogated the VEGF-induced phosphorylation of VEGFR2 and the expression of Fos (c-fos), a target gene of VEGF signalling²³ (Fig. 3i–k). In summary, these data demonstrate that in the OF in vivo and in endothelial cells in vitro, DMP1 inhibits VEGF signalling, which is a known driver of angiogenesis and osteogenesis¹⁹. Taken together with previously published results⁸, we suggest that the anti-angiogenic effect of DMP1 likely is not restricted to type H vessels in bone but it rather appears to act as a general inhibitor of angiogenesis at those sites where DMP1 is present.

DMP1 secretion correlates positively with body weight and coincides spatiotemporally with FAM20C upregulation

Hormones, especially sex hormones, are usually considered important regulators of bone growth and metabolism^24,25,26. Mice achieve puberty by approximately week 6, which coincides in time with the burst in DMP1 secretion. We noticed that the extent of DMP1 release at 5.5-6 weeks varied considerably between individual mice (cf. Fig. 1f). We also noted that in mice with lower body weight than average, the burst in DMP1 secretion had not yet taken place at 5.5–6 weeks. Inversely, littermates heavier than average featured already very advanced DMP1 release at this time point (Fig. 4a). Indeed, we found a positive correlation between body weight and the extent of DMP1 secretion (Fig. 4b). DMP1 is a highly phosphorylated protein and a substrate of FAM20C kinase that exclusively mediates the phosphorylation and subsequent secretion of DMP1 into ECM in vivo^11,27,28. Notably, in 6-week-old mice, we found more FAM20C, along with higher DMP1 accumulation, in the OF centre in comparison to the periphery, which was not the case in 4-week-old mice (Fig. 4c–e). In line with FAM20C acting upstream of DMP1, inducible Dmp1 deletion did not impair FAM20C upregulation at the age of 6 weeks (Supplementary Fig. 8a, b). These findings suggest that mechanical loading through increasing body weight or/and muscle contractions, directly or indirectly, controls DMP1 secretion along with FAM20C upregulation.

Mechanical loading enhances FAM20C kinase production and DMP1 secretion

To investigate whether FAM20C production was enhanced by mechanical loading, we cultured live 400-500-µm-thick slices of 4-week-old femurs and centrifuged them twice daily on 5 consecutive days (Fig. 5a). Loading via centrifugation caused an upregulation of intracellular FAM20C protein and enhanced DMP1 secretion (Fig. 5b, c and Supplementary Fig. 8c, d). The mechanically induced FAM20C upregulation apparently occurred hormone-independently in the cultured femur slices in vitro.

To study if body weight- and/or muscle force-associated mechanical loading controls FAM20C production also in vivo, we unloaded one hind limb in the mouse by cutting the sciatic and femoral nerves at the age of 4.5 weeks (Fig. 5d). This results in paralysis of the affected hind limb, hence releasing it from loading through muscle contraction as well as body weight with all weight going through the contralateral (normal) side, whereas both limbs were exposed to the same hormonal environment. Unloading by double neurectomy prevented FAM20C upregulation and DMP1 secretion at 5.5–6 weeks in almost all of the operated limbs, whilst preserving normal DMP1 release on the contralateral side (Fig. 5e, f and Supplementary Fig. 8e, f). Thus, mechanical loading enhances local FAM20C production and DMP1 secretion.

Mechanical forces trigger PIEZO1 to enhance FAM20C kinase production and DMP1 secretion

In our transcriptome analysis, we found four mechanoreceptors that osteoblasts could potentially express in the OF during late postnatal development: Trpv4, Kcnk2, Piezo1, Piezo2 (Supplementary Fig. 8g). Recently published studies show that in osteoblasts, mainly PIEZO1 and to a lesser extent PIEZO2 are key mechanosensors mediating anabolic effects of loading on bone^{29,30,31,32,33}. Thus, we tested whether PIEZO1 or PIEZO2 control Fam20c upregulation in osteoblasts by analysis of bones of 6-7-week-old mice with Piezo1 or Piezo2 deficiency in Dmp1-expressing cells (Piezo1^∆Dmp1 or Piezo2^∆Dmp1). FAM20C upregulation and the resulting burst in DMP1 secretion were lost exclusively in Piezo1^∆Dmp1 bones but normal in Piezo2^∆Dmp1 femurs (Fig. 6a–c, d–f and Supplementary Fig. 8h, i). Osteoblast maturation and abundance were not affected by Piezo1 deletion both at 4 and 7 weeks, as determined by the staining intensity and frequency of OSX⁺ cells in the OF (Supplementary Fig. 8j–n). 4-week-old Piezo1^∆Dmp1 bones featured similar levels of FAM20C and DMP1 as control bones indicating that PIEZO1 mediates the loading-induced upregulation of FAM20C around periadolescence, whereas basal FAM20C expression is PIEZO1 independent (Fig. 6g–i). In addition, we compared the phenotype of Piezo1^∆Dmp1 bones to control ones using von Kossa/van Gieson staining followed by histomorphometry at 7 weeks of age. Both assessments did not show significant differences between the groups at this age (Supplementary Fig. 8o, p). These data suggest that the enhanced FAM20C upregulation and DMP1 secretion in control bones versus Piezo1^∆Dmp1 bones is likely to be mediated directly by PIEZO1 and not by differences in bone maturation between the two groups. To provide additional evidence and validate the involvement of PIEZO1 in the proposed mechanism, we exposed femur slices from 4-week-old Piezo1^∆Dmp1 and Cre-negative (WT) littermate mice to loading via low-speed centrifugation as described (cf. Fig. 5a). Mechanical loading did not lead to upregulation of FAM20C or enhanced DMP1 release in Piezo1^∆Dmp1 femur slices, while it did so in Cre-negative (WT) samples (Fig. 6j and Supplementary Fig. 8q). To conclude, mechanical forces trigger PIEZO1 to enhance FAM20C expression in osteoblasts and subsequent DMP1 secretion into ECM during periadolescence.

**Fig. 6: Mechanoreceptor PIEZO1 drives FAM20C upregulation in osteoblasts and DMP1 secretion during periadolescence.**

Discussion

In this study, we identify a two-step molecular mechanism by which mechanical forces drive the maturation of bone and vessels at the end of adolescence (Fig. 7). First, body weight-associated mechanical loading triggers the mechanoreceptor PIEZO1 to enhance the production of FAM20C kinase in osteoblasts, which induces a burst in DMP1 secretion into ECM. Second, large amounts of extracellular DMP1 inhibit VEGF signalling in the OF and transform highly angiogenic type H vessels into quiescent type L vasculature to limit bone growth activity. In parallel, extracellular DMP1 leads to rapid matrix mineralisation and strengthening of long bones^9,34. Thus, the role of DMP1 in bone is multifaceted: it combines active inhibition of angiogenesis with mineralising activity. The discovered link between mechanical loading and FAM20C upregulation, a major kinase of the bone phosphoproteome^27,28, provides a mechanistic explanation for the well-known positive effect of loading on bone mineralisation³⁵. Furthermore, it may offer an additional mechanistic explanation for hormone-independent osteoporosis caused by lack of loading, for example in astronauts^36,37 or after long immobilisation of a fractured bone³⁸.

**Fig. 7: Molecular mechanism transforming type H into type L vessels to limit adolescent bone growth activity.**

Growth-promoting type H vessels have received much attention in the last years because of their substantial clinical potential^6,39. Our finding that inducible Dmp1 deletion led to increased vessel activity also in subchondral bone suggests that extracellular DMP1 may protect articular cartilage from vessel invasion, which accompanies neoinnervation of the tissue and sensation of pain in osteoarthritis patients^40,41. Moreover, DMP1-mediated inhibition of angiogenesis in bone may be useful in arresting growth of tumours including osteo- and chondrosarcomas^42,43,44. In future studies, we will address the precise molecular mechanism of how extracellular DMP1 inhibits VEGF signalling in type H endothelium. Particularly we will investigate if the inhibition of VEGFR3 expression is mediated directly by DMP1 or if it is an indirect effect via the inhibition of VEGFR2 signalling²¹.

Under the influence of systemic hormones, bones of male mice acquire higher mass and cortical thickness than females, which is already evident at early puberty⁴⁵ (3–5 weeks). As the weight of male mice is also greater than females at this age it is likely that the load perceived by individual osteoblasts in male and female bones is equivalent during growth according to the principles of isometric scaling⁴⁶. This may explain why the triggering of FAM20C upregulation and DMP1 secretion happens in both genders at the same age despite the difference in absolute body weight, and presumably after a key mechanical threshold has been reached. Our results suggest that mechanical force is a key player in the control of angiogenesis in bone and hence bone growth, acting locally on bones in addition to systemic sex hormones^24,25,26. However, the signalling downstream of PIEZO1 that results in enhanced FAM20C expression remains to be elucidated. Moreover, potential effects of sex hormones on PIEZO1 receptor sensitivity should be analysed in future studies.

Based on our finding that FAM20C upregulation is induced by loading and it happens first in the OF centre, we hypothesise that the central part of the OF is exposed to the highest mechanical strain since it is a spot where the loading from all four domes of the GP gets integrated. Moreover, there is no cortical bone underneath to absorb this loading (Supplementary Fig. 8r). The translation of these findings into two-legged humans, where the loading distribution and control of bone growth might be different, remains to be examined in follow-up studies.

The data presented here demonstrate that mechanical forces transmitted through PIEZO1 play a crucial role in the regulation of skeletal development and bone maturation via FAM20C-mediated extracellular DMP1 accumulation and its control of blood vessel subtypes in bone. This knowledge may be applied in bone healing studies⁴⁷, as well as in different kinds of bone pathologies featuring abnormal bone growth activity, including osteoarthritis, osteosarcoma and osteoporosis.

Methods

Mice

Tie2-GFP reporter mice (Tg(TIE2GFP)287Sato/J, Stock No: 003658) were purchased from Jackson Laboratory. For analysis of wild-type femurs, C57BL/6N and C57BL/6J mice were used. C57BL/6J mice were used for double neurectomy experiments. Dmp1^-/- and Dmp1^fl/fl mice were produced by microinjection of C57BL/6J zygotes with Cas9 protein and synthetic guide RNA gDMP#1 (TTGGGAAGATAACCGCTTAG) and gDMP#2 (CAGCTGAGGCGAGTACCACA). Thereby Exon 6, which encodes 80% of the DMP1 protein, was deleted to generate constitutive Dmp1-deficient mice (Supplementary Fig. 4). For inducible deletion of Dmp1, loxP sites were inserted at the site of the gRNA target sites flanking Exon 6 using a customised gene targeting vector: 500 bp upstream of Exon 6 and 500 bp downstream of the open reading frame (ORF). Dmp1-floxed mice were bred with Col1a2-CreERT2 mice, which were kindly provided by Prof. Dr. Thomas Blankenstein (Charité, Berlin). To induce Cre activity and gene deletion, 4-week-old offspring (both CreERT2^+/- and also CreERT2^-/- controls) was injected intraperitoneally with 50 mg/kg of tamoxifen (Sigma, T5648) for 5 consecutive days: P28-32. The resulting Dmp1^i∆OB (CreERT2^+/-Dmp1^fl/fl) mutants and control (CreERT2^-/-Dmp1^fl/fl) mice were killed on day P42 (6 weeks) and the bones were collected for analysis. Piezo1^fl/fl-Dmp1Cre and Piezo2^fl/fl-Dmp1Cre were described before²⁸. Cre-negative littermates were always used as controls. The vasculature phenotype of tamoxifen-injected CreERT2^-/- control mice was identical to regular WT mice (cf. Fig. 2a (Centre) to Fig. 2h (Control)). Thus, injection of tamoxifen as such did not affect the vasculature at the doses administered.

In all experiments, both genders were analysed together and no gender-specific differences were observed. All mice were bred under specific pathogen-free (SPF) conditions and treated according to the requirements of the German and UK legislation. Mice were kept in approved animal-care facilities and were housed 4–6 per cage in standard individually ventilated cages, maintained with a 12 h light/dark cycle at an ambient temperature of 21 °C and 45% to 65% relative humidity with wood shavings bedding and nesting material. Mice had ad libitum access to tap water and standard rodent chow (Altromin - LASQCdiet™ Rod16, Autoclavable, 10 mm). The age of mice, when they were used for the experiment was between 3 and 15 weeks, the precise age is always indicated in the text. All animal experiments were performed according to relevant laws and institutional guidelines (DRFZ, Berlin, Germany and University of Oxford, Oxford, UK) and were approved by German and UK animal ethics committees (LAGeSo license number G0175/19 and Project (Vincent) license as granted by the UK Home Office).

Laser microdissection

Tie2-GFP mice were killed via cervical dislocation at 4 weeks or 12 weeks of postnatal development. Femurs were immediately collected and frozen in SCEM medium using dry ice-isopropanol-hexane cooling bath. After freezing the blocks were kept at −80 °C until cryosectioning. The temperature of the cryostat chamber and the knife was −32 °C and -34 °C, respectively. For cryosectioning, type D knife for hard tissue was used. All surfaces including the knife were cleaned with RNaseZap before use. Cryosectioning was performed with Kawamoto’s films for LMD. To minimise RNA degradation, 12 µm sections were mounted into the metal frame directly after cutting and transported in the evaporating phase of liquid nitrogen. Laser microdissection was performed using Leica LMD7 device with fluorescent lamp for endothelial cell identification. To minimize the humidity during microdissection, constant flow of evaporating liquid nitrogen was applied. The microdissected pieces were collected into lysis buffer (ARCTURUS® PicoPure® RNA Isolation Kit). Approximately 50 pieces were collected from each sample. Immediately after collection, the Eppendorf tube containing the sample was briefly centrifuged and incubated at 42 °C for 10 min. Afterwards, it was vortex shortly and frozen on dry ice and kept at −80 °C until RNA isolation.

RNA sequencing and analysis

RNA was isolated using ARCTURUS® PicoPure® RNA Isolation Kit according to manufacturer’s instructions. Illumina libraries were generated using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech) and Nextera XT DNA Sample Preparation Kit (Illumina), with up to 10 ng of purified cDNA. The quality of synthesised cDNA was checked using Bioanalyzer (Agilent). For sequencing, Illumina NextSeq500 device was used generating 75 bp paired-end reads. Reads were mapped to the mouse mm10 genome using TopHat2 (PMID: 23618408) and Bowtie2 (PMID: 22388286) with default settings⁴⁸. Further analysis was done with R 3.4.0 using the default settings of the deseq2 package (PMID:25516281). To identify the genes with the highest significance, fold change and decent expression level, the following filtering strategy was applied: mean expression value >500, Log₂ FC > 0.66, P < 10⁻⁵. The heat map for selected genes was generated by plotting log2-transformed read counts.

Immunohistochemistry

Mouse bones were processed as described previously⁴⁹. Briefly, the bones were collected, cleaned from surrounding tissue and muscles, and immediately fixed in 4% freshly prepared ice-cold paraformaldehyde (PFA) for 4 h on ice. Afterwards, the bones were washed 3 times in ice-cold PBS followed by decalcification in 0.5 M EDTA solution (pH=7.5) for 24 h under constant agitation at 4 °C. Cryoprotection was done overnight in 20% sucrose and 2% PVP solution at 4 °C immediately after decalcification. Afterwards, 5 ml of embedding medium (8% gelatine, 20% sucrose and 2% PVP in PBS) was added per falcon tube and the bones were incubated in it for 45 min at 60 °C followed by embedding into cryomolds. The femurs were always oriented the same way with the anterior side facing the bottom of the mould. After embedding the samples were left at room temperature (RT) for 30 min for complete medium solidification. The blocks were kept at −80 °C in air-tight containers until cryosectioning. Cryosectioning was performed using NX70 ThermoFischer cryostat at −25 °C and −27 °C for chamber and blade, respectively. The disposable low-profile blades N35 for hard tissue (207500006, Feather) were used for cutting. 100 µm sections were generated and transferred onto Superfrost slides (J1800AMNT, Thermo Fisher Scientific). The slides were kept at −20 °C until staining. For immunostaining, bone sections were air-dried, permeabilized in 0.3% Triton X-100 for 15 min, blocked in 10% donkey serum at RT for 30 min, and incubated with the primary antibodies diluted in 5% donkey serum in PBS for 2 h at RT or overnight at 4 °C. After primary antibody incubation, sections were washed with PBS three times and incubated with corresponding secondary antibodies for 1 h at RT. Nuclei were counterstained with Hoechst (1:1000, 33342, Thermo Fischer Scientific). After incubation with secondary antibodies, sections were washed three times with PBS and mounted with Fluoromount-G medium (00-4958-02, Thermo Fischer Scientific). The list of used primary and secondary antibodies is provided in the Supplementary Information (Supplementary Table 1 and 2).

RNAScope

Freshly dissected mouse femurs were fixed in 4% freshly prepared PFA for 24 h at 4 °C followed by washings in PBS. Cryoprotection was performed via overnight incubations in 10, 20, and 30% sucrose at 4 °C. Bones were frozen in SCEM medium. The frozen blocks were kept at −80 °C until cryosectioning. Kawamoto cryofilms type 3C(16UF) were used for cryosectioning. The sections were air-dried in the cryotome for 15 min and stored at −80 °C in the slide box with molecular sieves inside. For RNAScope assay, sections were air-dried at RT for 5 min, washed in PBS for 5 min and baked on 60 °C hot plate for 30 min followed by additional fixation in 4% PFA at 4 °C for 15 min. Afterwards, the sections underwent 5 min dehydration steps in ethanol gradient: 50, 70, and 100% (x2). The sections were air-dried again for 5 min followed by 10 min incubation in hydrogen peroxide and washings in distilled water. The target retrieval buffer was prewarmed in the steamer for ~10 min up to 75 °C. The slides were submerged into it and left in the steamer for 10 min. The temperature in the steamer was 95 °C. Afterwards, the slides were washed in distilled water and dehydrated in 100% ethanol for 10–15 s. The slides were air-dried for 5 min at RT or overnight. Protease III was applied, and the slides were incubated in the Hybez Oven for 30 min followed by washings in distilled water. The rest of the RNAScope procedure was performed according to the standard RNAScope® Multiplex Fluorescent v2 Assay protocol.

Double neurectomy

Mice received pre-emptive pre-operative subcutaneous analgesia. In all, 5 mm pieces of sciatic and femoral nerves of the right hind limb were cut out in 4-4.5-week-old C57BL/6J mice under general anaesthesia using isoflurane. After surgery, the mice were housed without tubes and on soft bedding. It was observed that all mice dragged the operated right limb without stepping on it. Mice were killed at the age of 5.5–6 weeks and the bones were collected for further analysis. The contralateral left femur was always used as control and was analysed in pair with the operated unloaded right femur.

In vitro culture of bone slices

Femurs were collected from 4-week-old C57BL/6 J mice. Half of the bone together with knee metaphysis was cut and frozen in 2.5% agarose on ice with subsequent cooling in liquid nitrogen. Under the sterile work bench, the frozen block was fixed on the sample holder for cryosectioning, which was placed on dry ice. 400−500 µm-thick slices of femur were produced using sterile disposable blade for hard tissue. Every slice was placed into a well of a 96-well PRC plate filled with StemMACS™ OsteoDiff Media (Miltenyi Biotec). The position of the slice is depicted in Fig. 4a. At least two slices per femur were generated, each of them was placed into a separate plate. The plates were kept at 37 °C with 5% CO₂ in a humidified atmosphere. One plate with slices was centrifuged twice daily at 19 g (the lowest possible speed of the centrifuge) at 37 °C for 30 min. The control plate was not subjected to centrifugation. The medium was changed every 2.5 days. After 5 days of culture, the slices were fixed in 4% PFA on ice for 30 min followed by washings in PBS and decalcification for 30 min in 0.5 M EDTA on the cooling element under constant agitation. Cryoprotection was done in 20% sucrose and 2% PVP solution in PBS at 4 °C overnight. The slices were frozen in embedding medium (8% gelatine, 20% sucrose and 2% PVP in PBS). 100 µm sections were prepared using the NX70 Cryotome (Thermo Fischer Scientific). The sections were stored at −20 °C until immunostaining. The staining was performed as described in the paragraph “Immunohistochemistry”. Paired analysis was done based on the slices derived from the same mouse.

Image acquisition

All immunofluorescent and RNAScope-multifluorescent stainings were acquired at high resolution with Zeiss LSM-880 confocal microscope using 20x or 63x objectives. All microscope settings including laser power were kept constant throughout the experiments within the same type of samples and same staining panels. All immunostained samples were always acquired in Z-stack mode followed by maximum intensity projection.

Image analysis

The images were processed and analysed using ImageJ and CellProfiler software, in compliance with Nature’s guide for digital images. Quantification of immunofluorescent images: first, the total intensity of a fluorescent staining in a region of 300 µm width and 150 µm depth (from the growth plate towards the caudal region) was measured. Next, the area occupied by the protein was calculated by creating a mask (cf. Supplementary Fig. 2). Then the normalised intensity was calculated by dividing the total intensity by the occupied area (DMP1, EMCN) or by the total cell density (FAM20C). When the total amount of a protein per region was analysed, we used the total intensity without further normalisation to the area occupied by it (DMP1, pVEGFR2, MMP9). Background subtraction using ImageJ software was always done prior to quantification.

For RNAScope-multifluorescent images, the central and peripheral regions of 600 µm × 300 µm area underneath the growth plate were analysed. A cell was counted as Runx2⁺ or Osx⁺ if it contained at least one bright dot of the corresponding probe at 200x magnification. The final figures were compiled using Adobe Illustrator 2021 software (version 25.0.1).

Endothelial cell culture

The mouse capillary endothelial cell line bEnd.3⁵⁰ was kindly provided by Prof. Dr. Alf Hamann (Charité, Berlin). The cells were cultured in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (Catalogue No. 30-2002) containing 10% FCS and 100 U/mL penicillin and 100 mg/mL streptomycin. The cells were maintained at 37 °C with 5% CO₂ in a humidified atmosphere. Cells were cultured with recombinant DMP1 (40 nM) for 4 or 24 h. At the end of this incubation time, VEGF (50 ng/ml) was added to the cells for 15-20 min with subsequent cell fixation for immunocytochemistry or RNA collection for qPCR.

Immunocytochemistry

bEnd.3 cells were cultured in 8- or 16-well slide chambers (Tissue Tek). By the end of the experiment, cells were fixed in 4% freshly prepared ice-cold PFA for 10 min. Afterwards, they were washed 3 times with PBS and blocked in PBS containing 10% FCS for 30 min. The primary antibodies were applied for 1 h at RT in PBS containing 5% FCS and 0.1% Tween-20. After primary antibody incubation, cells were washed 3 times with PBS-Tween-20 (0.1%) and incubated with appropriate secondary antibodies diluted in PBS containing Hoechst (1:1000) for 1 h at RT. Finally, the cells were washed again three times with PBS and mounted using Fluoromount-G medium (Thermo Fischer Scientific).

qPCR

mRNA of bEnd.3 cells was isolated with the NucleoSpin RNA XS kit (Macherey Nagel) according to the manufacturer’s protocol. cDNA was generated with TaqMan Reverse Transcription Reagents and quantified with PowerUp™ SYBR™ Green Master Mix using QuantStudio Real-Time PCR System (Thermo Fischer Scientific). The following primers were used: Fos (forward: CACTCCAAGCGGAGACAGAT, reverse: TCGGTGGGCTGCCAAAATAA), Gapdh (forward: AAGGTGATCCCAGAGCTGAA, reverse: CTGCTTCACCACCTTCTTGA). Fos expression was normalised to Gapdh.

Von Kossa staining

The samples were prepared as described in paragraph “RNAScope”. The staining was done according to the manufacturer’s instructions (Silver plating kit acc. to von Kossa, Merck Millipore, #100362). Briefly, 10 µm undecalcified femur sections were placed in distilled water for 1 min followed by incubation in silver nitrate solution for 5 min under direct sunlight. Afterwards, the samples were washed in running tap water for 3 min and incubated in sodium thiosulfate solution for 5 min. Then the samples were washed in running tap water for 1 min and mounted in water-based mounting medium. The images were acquired with Leica DM750 equipped with Leica ICC50 W Camera Module using LAS software and analysed with ImageJ software (version 2.0.0).

Histomorphometry

Dissected skeletons were fixed in 3.7% PBS-buffered formaldehyde for 18 hours, before they were stored in 80% ethanol. Bones were dehydrated in ascending alcohol concentrations and then embedded in methylmethacrylate for undecalcified histology as described⁵¹. Histological sections of 4 μm thickness from the sagittal plane were stained by von Kossa/van Gieson procedures as described⁵². Histomorphometry was performed according to the ASBMR guidelines using the OsteoMeasure histomorphometry system (OsteoMetrics, Decatur, GA, USA).

Statistics

Statistical analysis was carried out using GraphPad Prism software (version 8.0d and 8.4). Normal distribution was tested using Shapiro-Wilk and Kolmogorov–Smirnov tests. In case of normal distribution parametric two-tailed paired t-test or unpaired t-test were used. For non-Gaussian distribution, non-parametric tests were used: two-tailed Wilcoxon test for paired analysis and two-tailed Mann–Whitney test for unpaired analysis. P < 0.05 was considered significant. Mice with body weight that was >20% below the average weight of their littermates were excluded from the inducible Dmp1-deletion experiments. All image quantifications were done automatically upon manual blinded selection of central and peripheral OF regions of the same size. Sample numbers are indicated in figure legends. Reproducibility was ensured by several independent experiments.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

RNA sequencing data are available at gene expression omnibus (GEO) under the accession number GSE148804. Source data are provided with this paper. All other data supporting the key findings of this study are available from the corresponding authors upon request.

References

Berendsen, A. D. & Olsen, B. R. Bone development. Bone 80, 14–18 (2015).
Article CAS PubMed PubMed Central Google Scholar
Kusumbe, A. P., Ramasamy, S. K. & Adams, R. H. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature 507, 323–328 (2014).
Article ADS CAS PubMed PubMed Central Google Scholar
Ramasamy, S. K., Kusumbe, A. P., Wang, L. & Adams, R. H. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature 507, 376–380 (2014).
Article ADS CAS PubMed PubMed Central Google Scholar
Romeo, S. G. et al. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation. Nat. Cell Biol. 21, 430–441 (2019).
Article CAS PubMed Google Scholar
Ramasamy, S. K. et al. Regulation of hematopoiesis and osteogenesis by blood vessel–derived signals. Annu. Rev. Cell Dev. Biol. 32, 649–675 (2016).
Article CAS PubMed Google Scholar
Xu, R. et al. Targeting skeletal endothelium to ameliorate bone loss. Nat. Med. 24, 823–833 (2018).
Article CAS PubMed PubMed Central Google Scholar
Langen, U. H. et al. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat. Cell Biol. 19, 189–201 (2017).
Article CAS PubMed PubMed Central Google Scholar
Pirotte, S. et al. Dentin matrix protein 1 induces membrane expression of VE-cadherin on endothelial cells and inhibits VEGF-induced angiogenesis by blocking VEGFR-2 phosphorylation. Blood 117, 2515–2526 (2011).
Article CAS PubMed Google Scholar
Feng, J. Q. et al. Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism. Nat. Genet. 38, 1310–1315 (2006).
Article CAS PubMed PubMed Central Google Scholar
Feng, J. Q. et al. The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J. Dent. Res. 82, 776–780 (2003).
Article CAS PubMed Google Scholar
Liu, P. et al. Specific ablation of mouse Fam20C in cells expressing type i collagen leads to skeletal defects and hypophosphatemia. Sci. Rep. 7, 3590 (2017).
Article ADS PubMed PubMed Central CAS Google Scholar
Qin, C. et al. Evidence for the proteolytic processing of dentin matrix protein 1: Identification and characterization of processed fragments and cleavage sites. J. Biol. Chem. 278, 34700–34708 (2003).
Article CAS PubMed Google Scholar
Sun, Y. et al. Failure to process Dentin Matrix Protein 1 (DMP1) into fragments leads to its loss of function in osteogenesis. J. Biol. Chem. 285, 31713–31722 (2010).
Article CAS PubMed PubMed Central Google Scholar
Ye, L. et al. Dmp1-deficient mice display severe defects in cartilage formation responsible for a chondrodysplasia-like phenotype. J. Biol. Chem. 280, 6197–6203 (2005).
Article CAS PubMed Google Scholar
Lorenz-Depiereux, B. et al. DMP1 mutations in autosomal recessive hypophosphatemia implicate a bone matrix protein in the regulation of phosphate homeostasis. Nat. Genet. 38, 1248–1250 (2006).
Article CAS PubMed PubMed Central Google Scholar
Rios, H. F. et al. DMP1 is essential for osteocyte formation and function. J. Musculoskelet. Neuronal Interact. 5, 325–327 (2005).
CAS PubMed Google Scholar
Gerber, H. P. et al. VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat. Med. 5, 623–628 (1999).
Article CAS PubMed Google Scholar
Maes, C. et al. Impaired angiogenesis and endochondral bone formation in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188. Mech. Dev. 111, 61–73 (2002).
Article CAS PubMed Google Scholar
Yang, Y. Q. et al. The role of vascular endothelial growth factor in ossification. Int. J. Oral. Sci. 4, 64–68 (2012).
Article CAS PubMed PubMed Central Google Scholar
Tammela, T. et al. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Nature 454, 656–660 (2008).
Article ADS CAS PubMed Google Scholar
Zarkada, G., Heinolainen, K., Makinen, T., Kubota, Y. & Alitalo, K. VEGFR3 does not sustain retinal angiogenesis without VEGFR2. Proc. Natl Acad. Sci. USA 112, 761–766 (2015).
Article ADS CAS PubMed PubMed Central Google Scholar
Tammela, T. et al. VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling. Nat. Cell Biol. 13, 1202–1213 (2011).
Article CAS PubMed PubMed Central Google Scholar
Holmes, D. I. R. & Zachary, I. Placental growth factor induces FosB and c-Fos gene expression via Flt-1 receptors. FEBS Lett. 557, 93–98 (2004).
Article CAS PubMed Google Scholar
Nilsson, O., Marino, R., de Luca, F., Phillip, M. & Baron, J. Endocrine regulation of the growth plate. Horm. Res. 64, 157–165 (2005).
CAS PubMed Google Scholar
Khosla, S. & Monroe, D. G. Regulation of bone metabolism by sex steroids. Cold Spring Harb. Perspect. Med. 8, a031211 (2018).
Article PubMed PubMed Central CAS Google Scholar
Chagin, A. S. et al. Estrogen receptor-β inhibits skeletal growth and has the capacity to mediate growth plate fusion in female mice. J. Bone Miner. Res. 19, 72–77 (2004).
Article CAS PubMed Google Scholar
Tagliabracci, V. S. et al. Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. Science 336, 1150–1153 (2012).
Article ADS CAS PubMed PubMed Central Google Scholar
Tagliabracci, V. S. et al. A single kinase generates the majority of the secreted phosphoproteome. Cell 161, 1619–1632 (2015).
Article CAS PubMed PubMed Central Google Scholar
Li, X. et al. Stimulation of Piezo1 by mechanical signals promotes bone anabolism. eLife 8, e49631 (2019).
Article PubMed PubMed Central Google Scholar
Sun, W. et al. The mechanosensitive Piezo1 channel is required for bone formation. eLife 8, e47454 (2019).
Article CAS PubMed PubMed Central Google Scholar
Zhou, T. et al. Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-β-catenin. eLife 9, e52779 (2020).
Article CAS PubMed PubMed Central Google Scholar
Wang, L. et al. Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk. Nat. Commun. 11, 282 (2020).
Article ADS CAS PubMed PubMed Central Google Scholar
Hendrickx, G. et al. Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation. J. Bone Miner. Res. 36, 369–384 (2020).
Article PubMed CAS Google Scholar
Ye, L. et al. Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J. Biol. Chem. 279, 19141–19148 (2004).
Article CAS PubMed Google Scholar
Ozcivici, E. et al. Mechanical signals as anabolic agents in bone. Nat. Rev. Rheumatol. 6, 50–59 (2010).
Article CAS PubMed PubMed Central Google Scholar
Lang, T. et al. Cortical and trabecular bone mineral loss from the spine and hip in long-duration spaceflight. J. Bone Miner. Res. 19, 1006–1012 (2004).
Article PubMed Google Scholar
Keyak, J. H., Koyama, A. K., LeBlanc, A., Lu, Y. & Lang, T. F. Reduction in proximal femoral strength due to long-duration spaceflight. Bone 44, 449–453 (2009).
Article CAS PubMed Google Scholar
Ceroni, D. et al. Effects of cast-mediated immobilization on bone mineral mass at various sites in adolescents with lower-extremity fracture. J. Bone Joint. Surg. - Ser. Am. 94, 208–216 (2012).
Article Google Scholar
Peng, Y., Wu, S., Li, Y. & Crane, J. L. Type H blood vessels in bone modeling and remodeling. Theranostics 10, 426–436 (2020).
Article CAS PubMed PubMed Central Google Scholar
Suri, S. et al. Neurovascular invasion at the osteochondral junction and in osteophytes in osteoarthritis. Ann. Rheum. Dis. 66, 1423–1428 (2007).
Article PubMed PubMed Central Google Scholar
Vincent, T. L. Peripheral pain mechanisms in osteoarthritis. Pain 161, 138–146 (2020).
Article Google Scholar
Duffaud, F. et al. Efficacy and safety of regorafenib in adult patients with metastatic osteosarcoma: a non-comparative, randomised, double-blind, placebo-controlled, phase 2 study. Lancet Oncol. 20, 120–133 (2019).
Article CAS PubMed Google Scholar
Davis, L. E. et al. Randomized double-blind phase II study of regorafenib in patients with metastatic osteosarcoma. J. Clin. Oncol. 37, 1424–1431 (2019).
Article CAS PubMed PubMed Central Google Scholar
Chow, W. et al. Results of a prospective phase 2 study of pazopanib in patients with surgically unresectable or metastatic chondrosarcoma. Cancer 126, 105–111 (2020).
Article CAS PubMed Google Scholar
Callewaert, F. et al. Sexual dimorphism in cortical bone size and strength but not density is determined by independent and time-specific actions of sex steroids and IGF-1: Evidence from pubertal mouse models. J. Bone Miner. Res. 25, 617–626 (2010).
Article CAS PubMed Google Scholar
Stern, T. et al. Isometric scaling in developing long bones is achieved by an optimal epiphyseal growth balance. PLoS Biol. 13, e1002212 (2015).
Article PubMed PubMed Central CAS Google Scholar
McDermott, A. M. et al. Recapitulating bone development through engineered mesenchymal condensations and mechanical cues for tissue regeneration. Sci. Transl. Med. 11, eaav7756 (2019).
Article CAS PubMed PubMed Central Google Scholar
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012).
Article CAS PubMed PubMed Central Google Scholar
Kusumbe, A. P., Ramasamy, S. K., Starsichova, A. & Adams, R. H. Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue. Nat. Protoc. 10, 1904–1914 (2015).
Article CAS PubMed Google Scholar
Sikorski, E. E., Hallmann, R., Berg, E. L. & Butcher, E. C. The Peyer’s patch high endothelial receptor for lymphocytes, the mucosal vascular addressin, is induced on a murine endothelial cell line by tumor necrosis factor-alpha and IL-1. J. Immunol. 151, 5239–5250 (1993).
CAS PubMed Google Scholar
Schinke, T. et al. Impaired gastric acidification negatively affects calcium homeostasis and bone mass. Nat. Med. 15, 674–681 (2009).
Article CAS PubMed Google Scholar
Dempster, D. W. et al. Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J. Bone Miner. Res. 28, 2–17 (2013).
Article PubMed Google Scholar

Download references

Acknowledgements

We thank Prof. Dr. T. Blankenstein for providing Col1-CreERT2 mice; Prof. Dr. A. Hamann for providing bEnd.3 cell line; Dr. G. Heinz and K. Lehmann for help with cDNA library preparation; V. Holecska, I. Panse and C. Rüster for technical assistance; M. Drabkina for advice on RNAScope technique; and Dr. P. Saikali, Dr. P. Shen, N. Duran, V. Plajer, and Dr. C. Helmstetter for advice and consultation. This work was supported by the Willy Robert Pitzer Foundation (Osteoarthritis Research Program, to M.L.), the state of Berlin and the “European Regional Development Fund” (ERDF 2014–2020, EFRE 1.8/11, to M.F.M.), the Leibniz Association (Leibniz Collaborative Excellence, TargArt to M.F.M.), Deutsche Forschungsgemeinschaft (grant no. AM 103/31-1, to T.S.), Centre for OA Pathogenesis Versus Arthritis (grant no. 21621, to T.L.V.). M.D. and T.M.B. were fellows of the International Max Planck Research School for Infectious Diseases and Immunology.

Funding

Open Access funding enabled and organized by Projekt DEAL.

Author information

Authors and Affiliations

Pitzer Laboratory of Osteoarthritis Research, German Rheumatism Research Centre (DRFZ), a Leibniz Institute, Berlin, Germany
Maria Dzamukova, Tobias M. Brunner & Max Löhning
Experimental Immunology and Osteoarthritis Research, Department of Rheumatology and Clinical Immunology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany
Maria Dzamukova, Tobias M. Brunner & Max Löhning
Centre for Osteoarthritis Pathogenesis Versus Arthritis, Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK
Jadwiga Miotla-Zarebska & Tonia L. Vincent
Therapeutic Gene Regulation, Regine von Ramin Lab Molecular Rheumatology, German Rheumatism Research Centre (DRFZ), a Leibniz Institute, Berlin, Germany
Frederik Heinrich & Mir-Farzin Mashreghi
Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Laura Brylka & Thorsten Schinke
BIH Center for Regenerative Therapies (BCRT), Charité – Universitätsmedizin Berlin, Berlin, Germany
Mir-Farzin Mashreghi
Tissue and Tumour Microenvironments Group, University of Oxford, Oxford, UK
Anjali Kusumbe
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany
Ralf Kühn

Authors

Maria Dzamukova
View author publications
You can also search for this author in PubMed Google Scholar
Tobias M. Brunner
View author publications
You can also search for this author in PubMed Google Scholar
Jadwiga Miotla-Zarebska
View author publications
You can also search for this author in PubMed Google Scholar
Frederik Heinrich
View author publications
You can also search for this author in PubMed Google Scholar
Laura Brylka
View author publications
You can also search for this author in PubMed Google Scholar
Mir-Farzin Mashreghi
View author publications
You can also search for this author in PubMed Google Scholar
Anjali Kusumbe
View author publications
You can also search for this author in PubMed Google Scholar
Ralf Kühn
View author publications
You can also search for this author in PubMed Google Scholar
Thorsten Schinke
View author publications
You can also search for this author in PubMed Google Scholar
Tonia L. Vincent
View author publications
You can also search for this author in PubMed Google Scholar
Max Löhning
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

M.D. and M.L. designed the experiments, interpreted the results and wrote the manuscript. T.M.B. helped with the generation of the transgenic mouse lines and in vivo experiments and provided technical expertise. J.M.Z. performed and T.L.V. supervised double neurectomy experiments. F.H. and M.F.M. provided transcriptome analysis. L.B. and T.S. provided Piezo1^∆Dmp1 and Piezo2^∆Dmp1 samples. A.K., T.S., and T.L.V. provided expertise and advice. R.K. designed and generated Dmp1^-/- and Dmp1^fl/fl mice. M.D. performed all other experiments and analysed the data. All authors reviewed and edited the manuscript.

Corresponding authors

Correspondence to Maria Dzamukova or Max Löhning.

Ethics declarations

Competing interests

The authors declare no competing interests.

Peer review

Peer review information

Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work. Peer reviewer reports are available.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Peer Review File

Supplementary Information

Reporting Summary

Source data

Source Data

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Dzamukova, M., Brunner, T.M., Miotla-Zarebska, J. et al. Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth. Nat Commun 13, 3059 (2022). https://doi.org/10.1038/s41467-022-30618-8

Download citation

Received: 07 October 2021
Accepted: 02 May 2022
Published: 01 June 2022
DOI: https://doi.org/10.1038/s41467-022-30618-8

This article is cited by

High expression of SRSF1 facilitates osteosarcoma progression and unveils its potential mechanisms
- Shuqi Li
- Xinyi Huang
- Qinghua Cao
BMC Cancer (2024)
Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis
- Cláudio Rodrigues Rezende Costa
- Rym Chalgoumi
- Ana Carolina Acevedo
Scientific Reports (2024)
Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs)
- Nityanand Prakash
- Jiseong Kim
- Soo-Hong Lee
Biomaterials Research (2023)
Generation of bicistronic Dmp1-Cre knock-in mice using a self-cleaving 2A peptide
- Takashi Nakamura
- Sayako Honda
- Makoto Suematsu
Journal of Bone and Mineral Metabolism (2023)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.