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A clinically relevant murine model of septic arthritis via direct inoculation of methicillin-resistant Staphylococcus aureus into the knee joint is described. This protocol details methods for serum, synovial fluid and knee joint tissue analysis that more closely mimic the workup of septic arthritis in human patients.
Rats undergoing the stress-enhanced fear learning procedure are usually housed in social isolation and exposed to a trauma-like experience of 15 massed electric footshocks. By showing that group-housed rats receiving fewer and lower-magnitude electric shocks still exhibit PTSD-relevant changes, this new study presents refinements of the procedure to reduce potential animal pain.
Sound levels, vibration, temperature, humidity and luminance were recorded in an animal facility that houses nonhuman primates; the findings may be used to characterize environmental conditions in similar primate facilities and inform best practices for nonhuman primate research and care.
Giannuzzi et al. present an integrated analysis of clinical features and exome and RNA sequencing data in a cohort of dogs with diffuse large B-cell lymphoma to better define the genetic landscape of this tumor and identify multiple mutations associated with the outcome.
In this article, the authors identify LanCL1 as a major male germ cell-specific antioxidant gene. Using different mouse models, they show that LanCL1 deficiency results in spermatozoal oxidative damage and impaired male fertility, while LanCL1 transgene expression protects mice against high-fat-diet-induced oxidative damage and subfertility.
This protocol describes an in vivo cartilage formation assay. Human or bovine articular chondrocytes injected in nude mice form cartilage organoids that can be used for the screening of molecules that promote cartilage formation.
In this article, Henry et al. examine how the geometry, size, opacity and depth of test chambers influence common behavioral responses in Artemia franciscana.
This protocol details the procedures for safe cardiac catheterization in Landrace pigs, basic electrophysiological assessment of conduction properties, induction of atrial fibrillation and development of a myocardial infarction model.