Nature Methods - CURRENT ISSUE : December 2008 - Vol 5 No 12
- Mapping genomic loci in the yeast nucleus
- Human protein factory: a resource
- Perspective on improving next-gen sequencing
Latest highlights
ANNOUNCEMENT
Method of the Year 2008!
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ADVANCE ONLINE PUBLICATION
Sequencing for CNVs
Article by Chiang et al.Massively parallel sequencing is a precise way to analyze copy-number variations given the right computational tools. Lander and colleagues present an algorithm to facilitate the detection and fine mapping of copy-number gains and losses from millions of short sequence reads.
RESEARCH HIGHLIGHTS
Engineering protein chimeras
Hotwiring protein regulationFREE
Researchers use an algorithm to identify residues involved in allosteric regulation and apply this information to construct protein chimeras linked through their allosteric sites; activation of an 'input' domain triggers a response of the 'output' domain. The findings may yield insights into protein evolution.
ADVANCE ONLINE PUBLICATION
Inhibiting microRNAs in vivo
Brief Communication by Gentner et al.To study microRNA function in vivo, Naldini and colleagues optimize lentivirally driven expression of microRNA target sequences in mice. They show dose-dependent inhibition of microRNA-mediated regulation of reporter constructs and of natural microRNA targets, and can phenocopy a microRNA knockout in vivo.
RESEARCH HIGHLIGHTS
Detecting proteins with 2DIR
Optical proteomicsFREE
Researchers have developed an all-optical protein identification platform based on amino acid composition analysis by two-dimensional infrared spectroscopy. The method shows promise for quantifying protein post-translational modifications.
Application Notes
Transiently produced recombinant proteins to speed up the decision-making process and limit risks
Go to the Application Notes database
See also this month:
Application Notes from Carestream Molecular Imaging, Olympus, Thermo Fisher Scientific, ProFact Proteomics Inc., cisbio, and Metabolon.