Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
This Perspective offers an overview of organic dyes commonly used as fluorescent labels and gives a chemist’s insight into their benefits and peculiarities.
Software solutions pGlyco3 and StrucGP both aim to better assign the glycan part of a glycopeptide beyond simple glycosyl composition, but they differ in their strategies, their requirement for a glycan library and their applicability to O-glycopeptides.
The MISpheroID knowledgebase records and organizes experimental parameters from thousands of cancer spheroid experiments, revealing heterogeneity and a lack of transparency in key spheroid research reporting practices.
Technological innovations in optical object recognition and high-throughput ultrasensitive mass spectrometry are enabling subcellular metabolomics and peptidomics, providing unprecedented opportunities to study small-molecule mediators of cellular function with important implications in health and disease.
Dynamic mass photometry, a method based on optical imaging of unlabeled proteins, enables direct observation and tracking of single-protein interactions on lipid membranes.
This Perspective describes advances in computer science that enable the integration of deep learning with traditional knowledge-based modeling in biological sciences, and discusses how such integration might overcome the challenges of modeling sparse, incomplete and noisy experimental data.
This Review describes the state of the art in imaging extracellular vesicles in animals to study their release, biodistribution and uptake, and covers labeling strategies, microscopy methods and discoveries made in model organisms.
This Review describes spatial omics and multiplexed imaging technologies and their current and future impact in studying tumor heterogeneity and cancer biology.
A four-dimensional single-cell atlas of transcription factor expression in Caenorhabditis elegans allows the identification of novel regulators of embryo development and the generation of molecular models of cell fate specification.