Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
The procedure guides inexperienced users interested in handling spatial omics data in a Python environment to streamline data analysis and to facilitate benchmarking analysis via the spatial omics database.
Mapping of chromatin states at single-cell resolution is still challenging. This protocol describes nano-CT, a novel method to simultaneously characterize up to three epigenetic modalities at single-cell resolution.
A protocol extension describing the purification of prenatal human brain endothelial and mural cells with FACS and their utilization in downstream applications, including cell culture, organoid transplantation and single-cell transcriptomics.
The detailed design and fabrication of small-scale magnetic soft-bodied robots with multimodal locomotion capability, including the processes required for locomotion control and optimization.
The authors describe a high-throughput screening strategy that can be carried out anaerobically for studying the effects of drugs in vitro on individual gut microbes or microbial communities created synthetically or obtained from stool samples.
This protocol presents a comprehensive workflow for the implementation of whole-genome sequencing in routine tumor diagnostics, exemplified by the pipeline used in the Netherlands Cancer Institute.
The recently available 28.2 Tesla nuclear magnetic resonance spectrometers (1.2 GHz 1H frequency) can be used to characterize highly flexible proteins and protein regions. This protocol explains how to setup 13C nuclear magnetic resonance experiments to achieve optimal results.
An automated workflow for culturing human induced pluripotent stem cells expressing fluorescently tagged proteins, and seeding them on 96-well optical-grade, glass-bottom plates for high-quality, live-cell three-dimensional microscopy on a large scale.
A protocol detailing the labeling and identification of cell- and subcompartment-specific proteins found within intact astrocytes and neurons in vivo using the proximity-dependent biotinylation system BioID2.
A protocol describing how to implant head-mounted jugular vein catheters in mice. This procedure facilitates systemic drug administration in a variety of experimental settings, including optical recording and manipulation of neuronal activities and behavioral tests.
Two-dimensional protein films, prepared via amyloid-like aggregation, can be made with nano- to meter-scale dimensions and features such as high interface adhesion, as well as tunable antibacterial, biomineralization and antifouling activity.
Tail-to-head terpene cyclization is an essential reaction for terpene synthesis, but it is difficult to achieve without an enzyme. This protocol describes the preparation and use of a hexameric resorcin[4]arene capsule as an artificial enzyme.
This protocol for the spatiotemporal control of RNA activity uses LicV, a synthetic, photoswitchable RNA-binding protein (RBP) that can bind to a specific RNA sequence in response to blue light irradiation, and provides an efficient and generalizable strategy for engineering photoswitchable RBPs.
This protocol describes a reproducible and reliable method for excisional skin wound healing assays in mice. The use of lineage-tracing assays to investigate the contribution of different cell populations to the repair process is also discussed.
CONIPHER is a computational framework for accurately inferring subclonal structure and the phylogenetic tree from multisample tumor sequencing, accounting for both copy number alterations and mutation errors.
This protocol describes barcoding bacteria for identification and quantification, a method for identifying and quantifying bacterial cells in microbiota samples based on the droplet-based barcoding and amplification of 16S rRNA genes from single bacterial cells.
Angstrom-scale two-dimensional channel devices can be assembled with precise control over their dimensions, as a single channel or hundreds of channels using layered crystals, and enable the measurement of angstrom-scale gas, ion and water fluidics.
Obtaining large, uniform crystals of polymer materials, including covalent organic frameworks, is challenging. This protocol describes a fast, effective approach to making crystalline two- and three-dimensional covalent organic frameworks using supercritical CO2 as the solvent.
This protocol presents a computational approach to scoring drug sensitivity that integrates multiple dose–response parameters into a single response metric and identifies differences in drug-response patterns between cancer cells and healthy control cells.