Featured
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| Open AccessA nanoscale reciprocating rotary mechanism with coordinated mobility control
Biological molecular motors convert chemical energy into mechanical motion by coupling catalytic reactions to large-scale structural transitions. Here, the authors report the design of a rotating DNA nanomechanism that comprises a camshaft whose rotary motion can be transformed into reciprocating large-scale transitions in the structure of the surrounding stator.
- Eva Bertosin
- , Christopher M. Maffeo
- & Hendrik Dietz
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Article
| Open AccessReversible and spatiotemporal control of colloidal structure formation
The self-assembly of colloidal particles can be applied to create new structures and materials in the framework of systems chemistry. The authors demonstrate the realization of autonomous oscillating structure formation on the meso-scale, relevant for systems relying on DNA interactions.
- H. Dehne
- , A. Reitenbach
- & A. R. Bausch
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Article
| Open AccessGrowth and site-specific organization of micron-scale biomolecular devices on living mammalian cells
Mesoscale molecular assemblies on the cell surface integrate information and amplify signals. Here the authors integrate DNA nanotubes in a controlled manner with mammalian cells to act as sheer stress meters.
- Sisi Jia
- , Siew Cheng Phua
- & Rebecca Schulman
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Article
| Open AccessSupercoiling and looping promote DNA base accessibility and coordination among distant sites
DNA supercoiling can result in underwinding with negative supercoiling or overwinding with positive supercoiling of the DNA double helix. Here the authors reveal insights into the dynamic relationship between DNA supercoiling-induced sequence-dependent disruptions to base pairing, DNA looping, and the shape of the DNA molecule.
- Jonathan M. Fogg
- , Allison K. Judge
- & Lynn Zechiedrich
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Article
| Open AccessDigital immunoassay for biomarker concentration quantification using solid-state nanopores
The concentration of a biomarker in solution can be determined by counting single molecules. Here the authors report a digital immunoassay scheme with solid-state nanopore readout to quantify a target protein and use this to measure thyroid-stimulating hormone from human serum.
- Liqun He
- , Daniel R. Tessier
- & Vincent Tabard-Cossa
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Article
| Open AccessProgramming ultrasensitive threshold response through chemomechanical instability
Controlling the threshold response in synthetic molecular structures is challenging. Here, the authors report on the buckling of ring-shaped DNA origami structures into twisted architectures via mechanical instability, induced by DNA intercalators.
- Young-Joo Kim
- , Junho Park
- & Do-Nyun Kim
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Article
| Open AccessAutonomous DNA nanostructures instructed by hierarchically concatenated chemical reaction networks
Integration and communication of distinct chemical reaction networks is a biological strategy for controlling dynamics of hierarchical structures. Here, the authors report ATP-fuelled autonomous DNA nanotube assembly regulated by DNA strand displacement reactions, which are induced and controlled by an upstream enzyme reaction network of concurrent ATP-mediated ligation and restriction of DNA components.
- Jie Deng
- & Andreas Walther
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Article
| Open AccessDNA dynamics and computation based on toehold-free strand displacement
Synthetic DNA constructs can to used to recognise and respond to input signals. Here the authors present complex DNA nanostructures with toehold-free strand displacement for generation of ON/OFF switches and Boolean gates.
- Hong Kang
- , Tong Lin
- & Bryan Wei
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Article
| Open AccessResponsive core-shell DNA particles trigger lipid-membrane disruption and bacteria entrapment
Lipid membrane disruption is often associated with disease but is also essential to a range of biosensing and therapeutic techniques. Here, the authors report on the development of DNA-based particles that, upon exposure to an external cue, can aggregate, disrupt lipid membranes, and arrest the motion of bacteria.
- Michal Walczak
- , Ryan A. Brady
- & Lorenzo Di Michele
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Article
| Open AccessA kinetically controlled platform for ligand-oligonucleotide transduction
Ligand-oligonucleotide interactions can integrate both small molecules and proteins into nucleic acid-based circuits. Here the authors design ligand-aptamer complexes to control strand-displacement reactions for versatile ligand transduction.
- Qiu-Long Zhang
- , Liang-Liang Wang
- & Liang Xu
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Article
| Open AccessTracking single particles for hours via continuous DNA-mediated fluorophore exchange
The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.
- Florian Stehr
- , Johannes Stein
- & Petra Schwille
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Article
| Open AccessA synthetic tubular molecular transport system
DNA origami can be used to control the movement of nanoscale assemblies. Here the authors construct multiple-micrometer-long hollow DNA filaments through which DNA pistons move with micrometer-per-second speeds.
- Pierre Stömmer
- , Henrik Kiefer
- & Hendrik Dietz
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Article
| Open AccessIntegration of photocatalytic and dark-operating catalytic biomimetic transformations through DNA-based constitutional dynamic networks
Nucleic acid-based constitutional dynamic networks (CDNs) enable control of various catalytic processes, but it is challenging to achieve intercommunication between different CDNs and by that mimic complex cell biology networks. Here, the authors report two CDNs that control the integration of photochemical and dark-operating processes, and show their intercommunication afforded by environmental components.
- Chen Wang
- , Michael P. O’Hagan
- & Itamar Willner
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Article
| Open AccessProton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells
Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E. coli to trigger a change in pH and control the deformation of giant unilamellar vesicles by simple illumination.
- Kevin Jahnke
- , Noah Ritzmann
- & Kerstin Göpfrich
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Article
| Open AccessDesigned and biologically active protein lattices
Organising proteins in 2D and 3D is needed to develop complex bimolecular materials for a range of applications. Here, the authors report the encapsulation of ferritin and apoferritin in DNA-based voxels with programmed assembly to generate both 2D and 3D protein lattices and demonstrate the retention of protein function.
- Shih-Ting Wang
- , Brian Minevich
- & Oleg Gang
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Article
| Open AccessDynamic self-assembly of compartmentalized DNA nanotubes
A major goal in Engineering Biology and Materials Science is the development of active, autonomous scaffolds that mimic those present in biological cells. Here the authors report a toolkit for programming the dynamic behaviour of nucleic acid scaffolds in minimal cell-like compartments.
- Siddharth Agarwal
- , Melissa A. Klocke
- & Elisa Franco
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Article
| Open AccessPromiscuous molecules for smarter file operations in DNA-based data storage
The molecular architecture of DNA data storage opens up interesting possibilities and functionalities. Here the authors leverage thermodynamics to control the access of different subsets of data in a file.
- Kyle J. Tomek
- , Kevin Volkel
- & Albert J. Keung
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Article
| Open AccessDimerization and oligomerization of DNA-assembled building blocks for controlled multi-motion in high-order architectures
Creation of high-order architectures using DNA devices is of interest for increasing the complexity of synthetic systems. Here, the authors, inspired by biological oligomers, create DNA dimers and oligomers that combining rotation and walking to make high-order systems with more complex conformational changes.
- Ling Xin
- , Xiaoyang Duan
- & Na Liu
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Article
| Open AccessDNA origami single crystals with Wulff shapes
DNA origami is a valuable tool for precise manipulation of molecules in a three dimensional manner, but the design and assembly of origami units into single crystals is challenging. Here, the authors report successful fabrication of DNA origami single crystals with Wulff shapes, and regulation of their shapes by changing the symmetry and binding modes of DNA origami building blocks.
- Yong Wang
- , Lizhi Dai
- & Ye Tian
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Article
| Open AccessAn alternative approach to nucleic acid memory
Encoding data in DNA is a promising approach to high density data storage. Here the authors present a prototype sequencing-free method that uses the spatial orientation of DNA strands with super-resolution microscopy readout.
- George D. Dickinson
- , Golam Md Mortuza
- & William L. Hughes
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| Open AccessLong- and short-ranged chiral interactions in DNA-assembled plasmonic chains
Here, the authors experimentally demonstrate chiral transfer over large distances up to 100 nm. They realise the coupling with an achiral nanosphere situated between a pair of distant gold nanorods arranged in a chiral fashion using DNA origami, and observe enhanced circular dichroism signals.
- Kevin Martens
- , Felix Binkowski
- & Tim Liedl
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Article
| Open AccessCellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications
Investigation of spatial organization and relationships of biomolecules in cellular nanoenvironments is necessary to understand essential biological processes, but methodologically challenging. Here, the authors report cellular macromolecules-tethered DNA walking indexing (Cell-TALKING) to probe the nanoenvironments of DNA modifications around histone post-translational modifications, and explore the nanoenvironments in different cancer cell lines and clinical specimens.
- Feng Chen
- , Min Bai
- & Yongxi Zhao
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| Open AccessRobust nucleation control via crisscross polymerization of highly coordinated DNA slats
For programmable DNA self-assembly, it is desirable to suppress spontaneous nucleation to enable all-or-nothing assembly of nanostructures far larger than a single DNA origami. Here the authors introduce crisscross polymerization of elongated slat monomers that engage beyond nearest neighbors, providing strictly seed-initiated nucleation of crisscross ribbons with distinct widths and twists.
- Dionis Minev
- , Christopher M. Wintersinger
- & William M. Shih
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Article
| Open AccessSpatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery
Controlled delivery of siRNA is important for potential clinical applications. Here, the authors report on the use of cascade hybridization of DNA hairpins with ATP aptamers in DNA crosslinked polymer nanoparticles for triggered release of siRNA and demonstrate anticancer applications.
- Feng Li
- , Wenting Yu
- & Dayong Yang
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Article
| Open AccessAddressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
Single-molecule fluorescence currently requires specialized imaging equipment due to the low signal of a single emitter. Here the authors introduce NanoAntennas with Cleared HOtSpots (NACHOS) to boost the signal sufficient for detection of a single emitter by a smartphone, opening the door to point-of-care applications.
- Kateryna Trofymchuk
- , Viktorija Glembockyte
- & Philip Tinnefeld
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Article
| Open AccessSelf-strengthening biphasic nanoparticle assemblies with intrinsic catch bonds
Catch bonds are protein–ligand interactions that exhibit enhancement of bond lifetime when subject to tensile force, which is a desirable yet elusive attribute for man-made nanoparticle interfaces. Here, the authors provide a nanoparticle design that can form catch bonds with tunable force-enhanced lifetimes under thermal excitations.
- Kerim C. Dansuk
- & Sinan Keten
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Article
| Open AccessUsing antibodies to control DNA-templated chemical reactions
DNA-templated synthesis takes advantage of sequence-specific hybridization to accelerate chemical reactions. Here, the authors present templated synthesis controlled through antibody-antigen interactions.
- Lorena Baranda Pellejero
- , Malihe Mahdifar
- & Francesco Ricci
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Article
| Open AccessRevealing the structures of megadalton-scale DNA complexes with nucleotide resolution
Precision design of DNA origami needs precision validation. Here, the authors developed cryo-EM methods for obtaining high resolution structural data and for constructing pseudo-atomic models in a semi-automated fashion, allowing for iterative nanodevice inspection and refinement.
- Massimo Kube
- , Fabian Kohler
- & Hendrik Dietz
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Article
| Open AccessFeedback regulation of crystal growth by buffering monomer concentration
Gaining control over crystallization processes is challenging. Herein, the authors describe a protocol for the controlled growth of DNA nanotubes by feedback regulation: the coupling of a reversible bimolecular monomer buffering reaction delivers the optimal monomer concentration and leads to reliable crystal growth in a simple manner.
- Samuel W. Schaffter
- , Dominic Scalise
- & Rebecca Schulman
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Article
| Open AccessComplex multicomponent patterns rendered on a 3D DNA-barrel pegboard
The design and optimisation of 3D DNA-origami can be a barrier to rapid application. Here the authors design barrel structure of stacked 2D double helical rings with complex surface patterns.
- Shelley F. J. Wickham
- , Alexander Auer
- & William M. Shih
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Article
| Open AccessDNA-assembled superconducting 3D nanoscale architectures
Fabrication of superconducting 3D nanoarchitectures, using standard nanofabrication methods, is challenging. Here, the authors demonstrate the fabrication of a nanostructured 3D superconducting array of Josephson junctions, exploiting self-assembled DNA origami lattices as a template.
- Lior Shani
- , Aaron N. Michelson
- & Oleg Gang
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Article
| Open AccessExpanding detection windows for discriminating single nucleotide variants using rationally designed DNA equalizer probes
The design of nucleic acid hybridisation probes is important for their use in DNA nanotechnology and biomedical applications. Here the authors use a DNA equalizer gate approach that expands the detection windows for improved sequence selectivity.
- Guan A. Wang
- , Xiaoyu Xie
- & Feng Li
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Article
| Open AccessRational design of DNA nanostructures for single molecule biosensing
A key attribute for modern healthcare is the ability to detect low concentrations of biomarkers. Here, the authors use nanopores and DNA origami with target-specific aptamers for detection of CRP.
- Mukhil Raveendran
- , Andrew J. Lee
- & Paolo Actis
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Article
| Open AccessATP-powered molecular recognition to engineer transient multivalency and self-sorting 4D hierarchical systems
There is interest in creating life-like behaviours in synthetic multicomponent systems. Here, the authors report on a modular DNA toolbox able to create transient, dynamic structures using ATP-driven molecular recognition in which multiple systems can run in parallel and across hierarchies.
- Jie Deng
- & Andreas Walther
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Article
| Open AccessCapturing transient antibody conformations with DNA origami epitopes
Understanding antibody-antigen interactions is important to deepen the understanding of immunology. Here, the authors report on the application of DNA origami structures for the controlled presentation of antigens to study antibody binding behaviours at room temperature.
- Ping Zhang
- , Xiaoguo Liu
- & Chunhai Fan
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Article
| Open AccessRational design of aptamer switches with programmable pH response
Previous design strategies for pH sensitive aptamers were not readily tunable across pH ranges. Here the authors present a general method to convert aptamers into pH-responsive switches using two orthogonal motifs.
- Ian A. P. Thompson
- , Liwei Zheng
- & H. Tom Soh
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Article
| Open AccessDesign of hidden thermodynamic driving for non-equilibrium systems via mismatch elimination during DNA strand displacement
Synthetic molecular systems require subtle control over their thermodynamics and reaction kinetics to implement features such as catalysis. Here the authors propose using mismatches in a DNA duplex to drive catalytic reactions forward whilst maintaining tight catalytic control.
- Natalie E. C. Haley
- , Thomas E. Ouldridge
- & Andrew J. Turberfield
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Article
| Open AccessColloidal crystal engineering with metal–organic framework nanoparticles and DNA
Colloidal crystals assembled from nanoscale building blocks are powerful designer materials with diverse functionalities. Here, the authors describe a colloidal crystal engineering strategy to prepare hierarchical structures from metal–organic framework nanoparticles and DNA which retain permanent porosity and catalytic activity.
- Shunzhi Wang
- , Sarah S. Park
- & Chad A. Mirkin
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Article
| Open AccessValence-programmable nanoparticle architectures
Assembling nanoparticles into precise architectures by controlling their positions in three-dimensional space is a major challenge in nanoscience. Here, the authors construct complex, preprogrammed clusters of DNA-encoded nanoparticles by coordinating them onto a DNA mesh wireframe.
- Sha Sun
- , Shize Yang
- & Oleg Gang
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Article
| Open AccessEncoding quantized fluorescence states with fractal DNA frameworks
Though DNA framework-based scaffolds for biomolecular assembly are attractive for bioimaging applications, realizing super-multiplex fluorescent amplifiers remains a challenge. Here, the authors report a topological engineering approach to designing fractal DNA frameworks for multiplexed amplifiers.
- Jiang Li
- , Jiangbing Dai
- & Chunhai Fan
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Article
| Open AccessProgramming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework
Tetrahedral DNA framework-enabled bulk enzyme heterojunctions have been used to program biosensor interfaces. Here, the authors use DNA tetrahedrons to tether enzymes of an enzymatic cascade to gold electrodes, hence raising them over the bulk solution, which led to improved kinetics and sensitivity.
- Ping Song
- , Juwen Shen
- & Chunhai Fan
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Article
| Open AccessImplementing digital computing with DNA-based switching circuits
DNA strand displacement reactions can be difficult to scale up for computational tasks. Here the authors develop DNA switching circuits that achieve high-speed computing with fewer molecules.
- Fei Wang
- , Hui Lv
- & Chunhai Fan
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Article
| Open AccessProgramming DNA origami patterning with non-canonical DNA-based metallization reactions
DNA origami has gained great interest for the creation of precise nanostructures. Here, the authors report on the creation of metalized DNA origami structured formed by metal condensation of DNA and nucleated metal growth on the formed DNA nanostructures.
- Sisi Jia
- , Jianbang Wang
- & Chunhai Fan
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Article
| Open AccessOrthogonal regulation of DNA nanostructure self-assembly and disassembly using antibodies
Antibodies are useful biomarkers and are emerging as powerful therapeutic tools. Here the authors report a rational strategy to orthogonally control assembly and disassembly of DNA-based nanostructures using specific IgG antibodies as molecular inputs.
- Simona Ranallo
- , Daniela Sorrentino
- & Francesco Ricci
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Article
| Open AccessDNA origami cryptography for secure communication
Biomolecular cyptography that exploits specific interactions could be used for data encryption. Here the authors use the folding of M13 DNA to encrypt information for secure communication.
- Yinan Zhang
- , Fei Wang
- & Chunhai Fan
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Article
| Open AccessA rotary plasmonic nanoclock
Current DNA-assembled nanophotonic devices can only reconfigure among random or few defined states. Here, the authors demonstrate a DNA-assembled rotary plasmonic nanoclock in which a rotor gold nanorod carries out directional and reversible 360° rotation transitioning among 16 well-defined configurations.
- Ling Xin
- , Chao Zhou
- & Na Liu
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Article
| Open AccessHigh-efficiency and integrable DNA arithmetic and logic system based on strand displacement synthesis
Current DNA computational systems are constrained by integration efficiency, device structures and limited functions. Here the authors design a DNA arithmetic logic unit that uses polymerase-mediated strand displacement.
- Haomiao Su
- , Jinglei Xu
- & Xiang Zhou
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Article
| Open AccessIndependent control of the thermodynamic and kinetic properties of aptamer switches
Aptamer switches are promising biotechnological tools but coupling of their affinity and temporal response limits their versatility. Here, the authors developed an intramolecular strand-displacement strategy that allows for independent fine-tuning of thermodynamics and kinetics of aptamer switches.
- Brandon D. Wilson
- , Amani A. Hariri
- & H. Tom Soh
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Article
| Open AccessSynthetic protein-conductive membrane nanopores built with DNA
Nanopores have a wide range of applications in the field of sensing. Here the authors report on synthetic nanopores made of DNA and designed for the transit of folded proteins across membranes to allow for biosensing.
- Tim Diederichs
- , Genevieve Pugh
- & Stefan Howorka